(Linear) (Six-base) MAPSORT of: KIEE5490.seq  Check: 7011  from: 1  to: 1029



KIEE5490.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     794    1029 
   Size:        794     235
 
AleI CACnn'nnGTG

Cuts at:      0     149     631    1029 
   Size:        149     482     398

  Fragments arranged by size: 

                 482     398     149
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     262    1029 
   Size:        262     767
 
BamHI G'GATC_C

Cuts at:      0     523    1029 
   Size:        523     506
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     517    1029 
   Size:        517     512
 
BglII A'GATC_T

Cuts at:      0     293    1029 
   Size:        293     736
 
BlpI GC'TnA_GC

Cuts at:      0     637    1029 
   Size:        637     392
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     172    1029 
   Size:        172     857
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310    1029 
   Size:        310     719
 
BsaWI w'CCGG_w

Cuts at:      0     737    1029 
   Size:        737     292
 
BseYI C'CCAG_C

Cuts at:      0    1021    1029 
   Size:       1021       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     633    1029 
   Size:        232     401     396

  Fragments arranged by size: 

                 401     396     232
 
BsmI GAATG_Cn'

Cuts at:      0     767    1029 
   Size:        767     262
 
BspEI T'CCGG_A

Cuts at:      0     737    1029 
   Size:        737     292
 
BspHI T'CATG_A

Cuts at:      0     488    1029 
   Size:        488     541
 
BsrDI GCAATG_nn'

Cuts at:      0     440     719    1029 
   Size:        440     279     310

  Fragments arranged by size: 

                 440     310     279
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     403    1029 
   Size:        403     626
 
BstYI r'GATC_y

Cuts at:      0     293     523     602    1029 
   Size:        293     230      79     427

  Fragments arranged by size: 

                 427     293     230      79
 
BtgI C'CryG_G

Cuts at:      0     362    1029 
   Size:        362     667
 
BtsI GCAGTG_nn'

Cuts at:      0     846    1029 
   Size:        846     183
 
EaeI y'GGCC_r

Cuts at:      0      86     437    1029 
   Size:         86     351     592

  Fragments arranged by size: 

                 592     351      86
 
EarI CTCTTCn'nnn_

Cuts at:      0     127     346    1000    1029 
   Size:        127     219     654      29

  Fragments arranged by size: 

                 654     219     127      29
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     794    1029 
   Size:        310     484     235

  Fragments arranged by size: 

                 484     310     235
 
FspI TGC'GCA

Cuts at:      0     143    1029 
   Size:        143     886
 
FspAI rTGC'GCAy

Cuts at:      0     143    1029 
   Size:        143     886
 
HincII GTy'rAC

Cuts at:      0      83    1029 
   Size:         83     946
 
MfeI C'AATT_G

Cuts at:      0     899    1029 
   Size:        899     130
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     501     620    1029 
   Size:        501     119     409

  Fragments arranged by size: 

                 501     409     119
 
MscI TGG'CCA

Cuts at:      0     439    1029 
   Size:        439     590
 
MslI CAynn'nnrTG

Cuts at:      0     149     232     361     631    1029 
   Size:        149      83     129     270     398

  Fragments arranged by size: 

                 398     270     149     129      83
 
MspA1I CmG'CkG

Cuts at:      0     513     694     772    1029 
   Size:        513     181      78     257

  Fragments arranged by size: 

                 513     257     181      78
 
NspI r_CATG'y

Cuts at:      0     142    1029 
   Size:        142     887
 
PfoI T'CCnGG_A

Cuts at:      0     287    1029 
   Size:        287     742
 
PstI C_TGCA'G

Cuts at:      0     383    1029 
   Size:        383     646
 
PvuII CAG'CTG

Cuts at:      0     513     772    1029 
   Size:        513     259     257

  Fragments arranged by size: 

                 513     259     257
 
SapI GCTCTTCn'nnn_

Cuts at:      0     346    1029 
   Size:        346     683
 
SfcI C'TryA_G

Cuts at:      0     379     948    1029 
   Size:        379     569      81

  Fragments arranged by size: 

                 569     379      81
 
SphI G_CATG'C

Cuts at:      0     142    1029 
   Size:        142     887
 
SspI AAT'ATT

Cuts at:      0      10    1029 
   Size:         10    1019
 
StuI AGG'CCT

Cuts at:      0     790    1029 
   Size:        790     239
 
TatI w'GTAC_w

Cuts at:      0     386    1029 
   Size:        386     643
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     517    1029 
   Size:        517     512
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    BamHI    BciVI    BglII     BlpI     BplI
     BpmI    BsaWI    BseYI     BsgI     BsmI    BspEI    BspHI    BsrDI
    BstXI    BstYI     BtgI     BtsI     EaeI     EarI  Eco57MI     FspI
    FspAI   HincII     MfeI     MmeI     MscI     MslI   MspA1I     NspI
     PfoI     PstI    PvuII     SapI     SfcI     SphI     SspI     StuI
     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI Bme1580I    BmgBI
     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI