(Linear) (Six-base) MAPSORT of: KIEE5489.seq  Check: 4150  from: 1  to: 1029



KIEE5489.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:03  ..

AccI GT'mk_AC

Cuts at:      0     614    1029 
   Size:        614     415
 
AleI CACnn'nnGTG

Cuts at:      0     829    1029 
   Size:        829     200
 
ApoI r'AATT_y

Cuts at:      0     124    1011    1029 
   Size:        124     887      18

  Fragments arranged by size: 

                 887     124      18
 
BfrBI ATG'CAT

Cuts at:      0     854    1029 
   Size:        854     175
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     421     794    1029 
   Size:        421     373     235

  Fragments arranged by size: 

                 421     373     235
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1017    1029 
   Size:       1017      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     715    1029 
   Size:        715     314
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98    1029 
   Size:         98     931
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     444     553    1029 
   Size:        444     109     476

  Fragments arranged by size: 

                 476     444     109
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       8      38    1029 
   Size:          8      30     991

  Fragments arranged by size: 

                 991      30       8
 
BseYI C'CCAG_C

Cuts at:      0     135     376    1029 
   Size:        135     241     653

  Fragments arranged by size: 

                 653     241     135
 
BspHI T'CATG_A

Cuts at:      0     497    1029 
   Size:        497     532
 
BsrGI T'GTAC_A

Cuts at:      0     675    1029 
   Size:        675     354
 
BstEII G'GTnAC_C

Cuts at:      0     214    1029 
   Size:        214     815
 
Bsu36I CC'TnA_GG

Cuts at:      0     329    1029 
   Size:        329     700
 
BtgI C'CryG_G

Cuts at:      0      27     443    1029 
   Size:         27     416     586

  Fragments arranged by size: 

                 586     416      27
 
EaeI y'GGCC_r

Cuts at:      0     464    1029 
   Size:        464     565
 
EarI CTCTTCn'nnn_

Cuts at:      0     187     844    1029 
   Size:        187     657     185

  Fragments arranged by size: 

                 657     187     185
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1017    1029 
   Size:       1017      12
 
EcoRI G'AATT_C

Cuts at:      0     124    1011    1029 
   Size:        124     887      18

  Fragments arranged by size: 

                 887     124      18
 
EcoRV GAT'ATC

Cuts at:      0     980    1029 
   Size:        980      49
 
HindIII A'AGCT_T

Cuts at:      0     649    1029 
   Size:        649     380
 
MscI TGG'CCA

Cuts at:      0     466    1029 
   Size:        466     563
 
MslI CAynn'nnrTG

Cuts at:      0     826     829    1029 
   Size:        826       3     200

  Fragments arranged by size: 

                 826     200       3
 
NcoI C'CATG_G

Cuts at:      0      27     443    1029 
   Size:         27     416     586

  Fragments arranged by size: 

                 586     416      27
 
NsiI A_TGCA'T

Cuts at:      0     856    1029 
   Size:        856     173
 
SfcI C'TryA_G

Cuts at:      0      56      78     744     890    1029 
   Size:         56      22     666     146     139

  Fragments arranged by size: 

                 666     146     139      56      22
 
SmlI C'TyrA_G

Cuts at:      0     113    1029 
   Size:        113     916
 
StyI C'CwwG_G

Cuts at:      0      27     279     443    1029 
   Size:         27     252     164     586

  Fragments arranged by size: 

                 586     252     164      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     368     403    1029 
   Size:        368      35     626

  Fragments arranged by size: 

                 626     368      35
 
TatI w'GTAC_w

Cuts at:      0     675    1029 
   Size:        675     354
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       8      40    1029 
   Size:          8      32     989

  Fragments arranged by size: 

                 989      32       8
 
XbaI T'CTAG_A

Cuts at:      0    1015    1029 
   Size:       1015      14
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     781    1029 
   Size:        781     248
 

 Enzymes that do cut:

     AccI     AleI     ApoI    BfrBI     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaXI    BseYI    BspHI    BsrGI   BstEII   Bsu36I     BtgI
     EaeI     EarI  Eco57MI    EcoRI    EcoRV  HindIII     MscI     MslI
     NcoI     NsiI     SfcI     SmlI     StyI    TaqII     TatI     TstI
     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BstYI  BstZ17I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XhoI     XmaI
     XmnI     ZraI