(Linear) (Six-base) MAPSORT of: KIEE5486.seq  Check: 417  from: 1  to: 1017



KIEE5486.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     562    1017 
   Size:        562     455
 
Acc65I G'GTAC_C

Cuts at:      0     827    1017 
   Size:        827     190
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     860    1017 
   Size:        860     157
 
AgeI A'CCGG_T

Cuts at:      0     416    1017 
   Size:        416     601
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     813    1017 
   Size:        813     204
 
ApoI r'AATT_y

Cuts at:      0      85    1017 
   Size:         85     932
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     819     852    1017 
   Size:        819      33     165

  Fragments arranged by size: 

                 819     165      33
 
BamHI G'GATC_C

Cuts at:      0     111     692    1017 
   Size:        111     581     325

  Fragments arranged by size: 

                 581     325     111
 
BanI G'GyrC_C

Cuts at:      0     661     827    1017 
   Size:        661     166     190

  Fragments arranged by size: 

                 661     190     166
 
BglI GCCn_nnn'nGGC

Cuts at:      0     476    1017 
   Size:        476     541
 
Bme1580I G_kGCm'C

Cuts at:      0     954    1017 
   Size:        954      63
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     516    1017 
   Size:        516     501
 
BsaWI w'CCGG_w

Cuts at:      0     416    1017 
   Size:        416     601
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      82     595    1017 
   Size:         82     513     422

  Fragments arranged by size: 

                 513     422      82
 
BseYI C'CCAG_C

Cuts at:      0     136     171    1017 
   Size:        136      35     846

  Fragments arranged by size: 

                 846     136      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     978    1017 
   Size:        427     551      39

  Fragments arranged by size: 

                 551     427      39
 
Bsp1286I G_dGCh'C

Cuts at:      0     954    1017 
   Size:        954      63
 
BspHI T'CATG_A

Cuts at:      0     458    1017 
   Size:        458     559
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     188     562    1017 
   Size:        188     374     455

  Fragments arranged by size: 

                 455     374     188
 
BsrFI r'CCGG_y

Cuts at:      0     126     416    1017 
   Size:        126     290     601

  Fragments arranged by size: 

                 601     290     126
 
BsrGI T'GTAC_A

Cuts at:      0     884    1017 
   Size:        884     133
 
BstEII G'GTnAC_C

Cuts at:      0     894    1017 
   Size:        894     123
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     159    1017 
   Size:        159     858
 
BstYI r'GATC_y

Cuts at:      0     111     692    1017 
   Size:        111     581     325

  Fragments arranged by size: 

                 581     325     111
 
Bsu36I CC'TnA_GG

Cuts at:      0     613     928    1017 
   Size:        613     315      89

  Fragments arranged by size: 

                 613     315      89
 
BtgI C'CryG_G

Cuts at:      0     281    1017 
   Size:        281     736
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     354    1017 
   Size:        354     663
 
EaeI y'GGCC_r

Cuts at:      0     425    1017 
   Size:        425     592
 
EarI CTCTTCn'nnn_

Cuts at:      0     856    1017 
   Size:        856     161
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     860    1017 
   Size:        860     157
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     686    1017 
   Size:        686     331
 
EcoRI G'AATT_C

Cuts at:      0      85    1017 
   Size:         85     932
 
KpnI G_GTAC'C

Cuts at:      0     831    1017 
   Size:        831     186
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     647     763    1017 
   Size:        269     378     116     254

  Fragments arranged by size: 

                 378     269     254     116
 
MscI TGG'CCA

Cuts at:      0     427    1017 
   Size:        427     590
 
MslI CAynn'nnrTG

Cuts at:      0     226     901    1017 
   Size:        226     675     116

  Fragments arranged by size: 

                 675     226     116
 
MspA1I CmG'CkG

Cuts at:      0     841    1017 
   Size:        841     176
 
NspI r_CATG'y

Cuts at:      0     309     380     393    1017 
   Size:        309      71      13     624

  Fragments arranged by size: 

                 624     309      71      13
 
PstI C_TGCA'G

Cuts at:      0     125    1017 
   Size:        125     892
 
PvuII CAG'CTG

Cuts at:      0     841    1017 
   Size:        841     176
 
SexAI A'CCwGG_T

Cuts at:      0     158    1017 
   Size:        158     859
 
SfcI C'TryA_G

Cuts at:      0     121     921    1017 
   Size:        121     800      96

  Fragments arranged by size: 

                 800     121      96
 
StyI C'CwwG_G

Cuts at:      0     254    1017 
   Size:        254     763
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     649    1017 
   Size:        649     368
 
TatI w'GTAC_w

Cuts at:      0     230     884    1017 
   Size:        230     654     133

  Fragments arranged by size: 

                 654     230     133
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     252     297     864    1017 
   Size:        252      45     567     153

  Fragments arranged by size: 

                 567     252     153      45
 
XmnI GAAnn'nnTTC

Cuts at:      0     913    1017 
   Size:        913     104
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AgeI     AlfI     ApoI     BaeI    BamHI
     BanI     BglI Bme1580I     BmrI    BsaWI    BseRI    BseYI     BsgI
 Bsp1286I    BspHI    BspMI    BsrFI    BsrGI   BstEII    BstXI    BstYI
   Bsu36I     BtgI     DrdI     EaeI     EarI  Eco57MI    EcoNI    EcoRI
     KpnI     MmeI     MscI     MslI   MspA1I     NspI     PstI    PvuII
    SexAI     SfcI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AhdI     AleI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI   BssHII    BssSI
   BstAPI    BstBI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EagI     EciI  EcoICRI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmaI     ZraI