(Linear) (Six-base) MAPSORT of: KIEE5485.seq  Check: 805  from: 1  to: 1014



KIEE5485.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     335     492     958    1014 
   Size:        335     157     466      56

  Fragments arranged by size: 

                 466     335     157      56
 
AflIII A'CryG_T

Cuts at:      0     851    1014 
   Size:        851     163
 
ApoI r'AATT_y

Cuts at:      0     360    1014 
   Size:        360     654
 
BamHI G'GATC_C

Cuts at:      0     221    1014 
   Size:        221     793
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     323    1014 
   Size:        323     691
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     252    1014 
   Size:        252     762
 
BclI T'GATC_A

Cuts at:      0     662    1014 
   Size:        662     352
 
BfrBI ATG'CAT

Cuts at:      0     334    1014 
   Size:        334     680
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       5    1014 
   Size:          5    1009
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     688    1014 
   Size:        688     326
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     564     647    1014 
   Size:        564      83     367

  Fragments arranged by size: 

                 564     367      83
 
BseYI C'CCAG_C

Cuts at:      0     754    1014 
   Size:        754     260
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     522    1014 
   Size:        522     492
 
BsmI GAATG_Cn'

Cuts at:      0     334    1014 
   Size:        334     680
 
BspHI T'CATG_A

Cuts at:      0     266    1014 
   Size:        266     748
 
BsrDI GCAATG_nn'

Cuts at:      0     146     738    1014 
   Size:        146     592     276

  Fragments arranged by size: 

                 592     276     146
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     524    1014 
   Size:        524     490
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1004    1014 
   Size:       1004      10
 
BstYI r'GATC_y

Cuts at:      0     221    1014 
   Size:        221     793
 
EarI CTCTTCn'nnn_

Cuts at:      0     951    1014 
   Size:        951      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       5     335     492     958    1014 
   Size:          5     330     157     466      56

  Fragments arranged by size: 

                 466     330     157      56       5
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     639     671     684     716    1014 
   Size:        639      32      13      32     298

  Fragments arranged by size: 

                 639     298      32      32      13
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     602     770     872    1014 
   Size:        602     168     102     142

  Fragments arranged by size: 

                 602     168     142     102
 
MspA1I CmG'CkG

Cuts at:      0     181    1014 
   Size:        181     833
 
NsiI A_TGCA'T

Cuts at:      0     336    1014 
   Size:        336     678
 
NspI r_CATG'y

Cuts at:      0     178     522     855    1014 
   Size:        178     344     333     159

  Fragments arranged by size: 

                 344     333     178     159
 
PasI CC'CwG_GG

Cuts at:      0     946    1014 
   Size:        946      68
 
PciI A'CATG_T

Cuts at:      0     851    1014 
   Size:        851     163
 
PstI C_TGCA'G

Cuts at:      0      75    1014 
   Size:         75     939
 
PvuII CAG'CTG

Cuts at:      0     181    1014 
   Size:        181     833
 
SapI GCTCTTCn'nnn_

Cuts at:      0     951    1014 
   Size:        951      63
 
SfcI C'TryA_G

Cuts at:      0      71    1014 
   Size:         71     943
 
SmlI C'TyrA_G

Cuts at:      0     703    1014 
   Size:        703     311
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     610    1014 
   Size:        610     404
 
XmnI GAAnn'nnTTC

Cuts at:      0     188    1014 
   Size:        188     826
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI    BamHI     BbsI    BciVI     BclI    BfrBI
     BpmI    BpuEI    BseRI    BseYI     BsgI     BsmI    BspHI    BsrDI
   BstAPI    BstXI    BstYI     EarI  Eco57MI    Hin4I     MmeI   MspA1I
     NsiI     NspI     PasI     PciI     PstI    PvuII     SapI     SfcI
     SmlI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI