(Linear) (Six-base) MAPSORT of: KIEE5484.seq  Check: 4117  from: 1  to: 1005



KIEE5484.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:02  ..

AccI GT'mk_AC

Cuts at:      0     634    1005 
   Size:        634     371
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     975    1005 
   Size:        395     580      30

  Fragments arranged by size: 

                 580     395      30
 
AleI CACnn'nnGTG

Cuts at:      0     274    1005 
   Size:        274     731
 
BanII G_rGCy'C

Cuts at:      0     733    1005 
   Size:        733     272
 
BglI GCCn_nnn'nGGC

Cuts at:      0     688    1005 
   Size:        688     317
 
BglII A'GATC_T

Cuts at:      0     461     994    1005 
   Size:        461     533      11

  Fragments arranged by size: 

                 533     461      11
 
Bme1580I G_kGCm'C

Cuts at:      0     281    1005 
   Size:        281     724
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     618    1005 
   Size:        618     387
 
Bpu10I CC'TnA_GC

Cuts at:      0     732    1005 
   Size:        732     273
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     560    1005 
   Size:        560     445
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     963    1005 
   Size:        963      42
 
BsiHKAI G_wGCw'C

Cuts at:      0     733    1005 
   Size:        733     272
 
Bsp1286I G_dGCh'C

Cuts at:      0     281     733    1005 
   Size:        281     452     272

  Fragments arranged by size: 

                 452     281     272
 
BsrDI GCAATG_nn'

Cuts at:      0     134    1005 
   Size:        134     871
 
BsrGI T'GTAC_A

Cuts at:      0     260    1005 
   Size:        260     745
 
BssSI C'ACGA_G

Cuts at:      0     701    1005 
   Size:        701     304
 
BstYI r'GATC_y

Cuts at:      0     461     994    1005 
   Size:        461     533      11

  Fragments arranged by size: 

                 533     461      11
 
BstZ17I GTA'TAC

Cuts at:      0     635    1005 
   Size:        635     370
 
BtsI GCAGTG_nn'

Cuts at:      0     950    1005 
   Size:        950      55
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     818     853    1005 
   Size:        818      35     152

  Fragments arranged by size: 

                 818     152      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     125    1005 
   Size:        125     880
 
EcoICRI GAG'CTC

Cuts at:      0     731    1005 
   Size:        731     274
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     975    1005 
   Size:        395     580      30

  Fragments arranged by size: 

                 580     395      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     822    1005 
   Size:        822     183
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       4      36     102     134     644     676     979    1005 
   Size:          4      32      66      32     510      32     303      26

  Fragments arranged by size: 

                 510     303      66      32      32      32      26       4
            
 
HincII GTy'rAC

Cuts at:      0     252    1005 
   Size:        252     753
 
MslI CAynn'nnrTG

Cuts at:      0     217     274     486     606    1005 
   Size:        217      57     212     120     399

  Fragments arranged by size: 

                 399     217     212     120      57
 
NspI r_CATG'y

Cuts at:      0     485    1005 
   Size:        485     520
 
SacI G_AGCT'C

Cuts at:      0     733    1005 
   Size:        733     272
 
SfcI C'TryA_G

Cuts at:      0     549     912     975    1005 
   Size:        549     363      63      30

  Fragments arranged by size: 

                 549     363      63      30
 
StyI C'CwwG_G

Cuts at:      0     363     387    1005 
   Size:        363      24     618

  Fragments arranged by size: 

                 618     363      24
 
TatI w'GTAC_w

Cuts at:      0     221     260    1005 
   Size:        221      39     745

  Fragments arranged by size: 

                 745     221      39
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     324    1005 
   Size:        324     681
 
XmnI GAAnn'nnTTC

Cuts at:      0     380    1005 
   Size:        380     625
 

 Enzymes that do cut:

     AccI     AcuI     AleI    BanII     BglI    BglII Bme1580I     BmrI
   Bpu10I     BsaI    BseRI  BsiHKAI Bsp1286I    BsrDI    BsrGI    BssSI
    BstYI  BstZ17I     BtsI    CspCI     EarI  EcoICRI  Eco57MI    EcoNI
    Hin4I   HincII     MslI     NspI     SacI     SfcI     StyI     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI    BmgBI
     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstBI   BstEII
    BstXI   Bsu36I     BtgI    BtgZI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EciI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI