(Linear) (Six-base) MAPSORT of: KIEE5483.seq  Check: 3029  from: 1  to: 1005



KIEE5483.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     560    1005 
   Size:        560     445
 
BamHI G'GATC_C

Cuts at:      0     781    1005 
   Size:        781     224
 
BanII G_rGCy'C

Cuts at:      0     233    1005 
   Size:        233     772
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     723    1005 
   Size:        723     282
 
BlpI GC'TnA_GC

Cuts at:      0     968    1005 
   Size:        968      37
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139    1005 
   Size:        139     866
 
Bpu10I CC'TnA_GC

Cuts at:      0     502    1005 
   Size:        502     503
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     565     731    1005 
   Size:        565     166     274

  Fragments arranged by size: 

                 565     274     166
 
BsaBI GATnn'nnATC

Cuts at:      0     571    1005 
   Size:        571     434
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538    1005 
   Size:        538     467
 
BsiHKAI G_wGCw'C

Cuts at:      0     551     599    1005 
   Size:        551      48     406

  Fragments arranged by size: 

                 551     406      48
 
Bsp1286I G_dGCh'C

Cuts at:      0     233     551     599    1005 
   Size:        233     318      48     406

  Fragments arranged by size: 

                 406     318     233      48
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     560    1005 
   Size:        560     445
 
BsrBI CCG'CTC

Cuts at:      0     758    1005 
   Size:        758     247
 
BsrDI GCAATG_nn'

Cuts at:      0     189     575     605    1005 
   Size:        189     386      30     400

  Fragments arranged by size: 

                 400     386     189      30
 
BsrGI T'GTAC_A

Cuts at:      0     161     236    1005 
   Size:        161      75     769

  Fragments arranged by size: 

                 769     161      75
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     866    1005 
   Size:        866     139
 
BstYI r'GATC_y

Cuts at:      0     781     997    1005 
   Size:        781     216       8

  Fragments arranged by size: 

                 781     216       8
 
Bsu36I CC'TnA_GG

Cuts at:      0     296    1005 
   Size:        296     709
 
BtgI C'CryG_G

Cuts at:      0     407    1005 
   Size:        407     598
 
BtsI GCAGTG_nn'

Cuts at:      0     167     491    1005 
   Size:        167     324     514

  Fragments arranged by size: 

                 514     324     167
 
EaeI y'GGCC_r

Cuts at:      0     257     305     404     428     887    1005 
   Size:        257      48      99      24     459     118

  Fragments arranged by size: 

                 459     257     118      99      48      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     385     671     811    1005 
   Size:        385     286     140     194

  Fragments arranged by size: 

                 385     286     194     140
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     724    1005 
   Size:        724     281
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139    1005 
   Size:        139     866
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     211    1005 
   Size:        211     794
 
EcoO109I rG'GnC_Cy

Cuts at:      0     300     765    1005 
   Size:        300     465     240

  Fragments arranged by size: 

                 465     300     240
 
EcoRV GAT'ATC

Cuts at:      0     630    1005 
   Size:        630     375
 
HaeII r_GCGC'y

Cuts at:      0      19    1005 
   Size:         19     986
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     742     773     774     805    1005 
   Size:        742      31       1      31     200

  Fragments arranged by size: 

                 742     200      31      31       1
 
MscI TGG'CCA

Cuts at:      0     259     307     406     430     889    1005 
   Size:        259      48      99      24     459     116

  Fragments arranged by size: 

                 459     259     116      99      48      24
 
MslI CAynn'nnrTG

Cuts at:      0     289     358     520     791    1005 
   Size:        289      69     162     271     214

  Fragments arranged by size: 

                 289     271     214     162      69
 
MspA1I CmG'CkG

Cuts at:      0     661    1005 
   Size:        661     344
 
NcoI C'CATG_G

Cuts at:      0     407    1005 
   Size:        407     598
 
PasI CC'CwG_GG

Cuts at:      0     112    1005 
   Size:        112     893
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     314    1005 
   Size:        314     691
 
PvuII CAG'CTG

Cuts at:      0     661    1005 
   Size:        661     344
 
SapI GCTCTTCn'nnn_

Cuts at:      0     671    1005 
   Size:        671     334
 
SfcI C'TryA_G

Cuts at:      0     840    1005 
   Size:        840     165
 
SmlI C'TyrA_G

Cuts at:      0     544     746    1005 
   Size:        544     202     259

  Fragments arranged by size: 

                 544     259     202
 
StuI AGG'CCT

Cuts at:      0     680    1005 
   Size:        680     325
 
StyI C'CwwG_G

Cuts at:      0     407     761    1005 
   Size:        407     354     244

  Fragments arranged by size: 

                 407     354     244
 
TatI w'GTAC_w

Cuts at:      0     161     236    1005 
   Size:        161      75     769

  Fragments arranged by size: 

                 769     161      75
 

 Enzymes that do cut:

     AarI    BamHI    BanII     BbsI     BlpI     BpmI   Bpu10I    BpuEI
    BsaBI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrGI
   BstAPI    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRV    HaeII    Hin4I     MscI     MslI
   MspA1I     NcoI     PasI    PflMI    PvuII     SapI     SfcI     SmlI
     StuI     StyI     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
  EcoICRI    EcoRI     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI