Restriction map for
pFN21AE5481
(Linear) (Six-base) MAPSORT of: KIEE5481.seq Check: 820 from: 1 to: 993
KIEE5481.orf
With 182 enzymes: SgfI *
January 29, 2014 12:02 ..
AccI GT'mk_AC
Cuts at: 0 259 970 993
Size: 259 711 23
Fragments arranged by size:
711 259 23
AleI CACnn'nnGTG
Cuts at: 0 781 993
Size: 781 212
AlwNI CAG_nnn'CTG
Cuts at: 0 847 993
Size: 847 146
ApaI G_GGCC'C
Cuts at: 0 855 993
Size: 855 138
AseI AT'TA_AT
Cuts at: 0 623 993
Size: 623 370
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 199 232 993
Size: 199 33 761
Fragments arranged by size:
761 199 33
BanI G'GyrC_C
Cuts at: 0 230 496 993
Size: 230 266 497
Fragments arranged by size:
497 266 230
BanII G_rGCy'C
Cuts at: 0 190 855 993
Size: 190 665 138
Fragments arranged by size:
665 190 138
BbsI GAAGACnn'nnnn_
Cuts at: 0 855 993
Size: 855 138
BbvCI CC'TCA_GC
Cuts at: 0 202 993
Size: 202 791
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 636 670 993
Size: 636 34 323
Fragments arranged by size:
636 323 34
Bme1580I G_kGCm'C
Cuts at: 0 233 275 855 993
Size: 233 42 580 138
Fragments arranged by size:
580 233 138 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 433 788 993
Size: 433 355 205
Fragments arranged by size:
433 355 205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 816 993
Size: 100 716 177
Fragments arranged by size:
716 177 100
Bpu10I CC'TnA_GC
Cuts at: 0 202 993
Size: 202 791
BsaAI yAC'GTr
Cuts at: 0 798 993
Size: 798 195
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 124 157 993
Size: 115 9 33 836
Fragments arranged by size:
836 115 33 9
BseYI C'CCAG_C
Cuts at: 0 157 770 847 993
Size: 157 613 77 146
Fragments arranged by size:
613 157 146 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 485 747 972 993
Size: 415 70 262 225 21
Fragments arranged by size:
415 262 225 70 21
BsiHKAI G_wGCw'C
Cuts at: 0 788 993
Size: 788 205
Bsp1286I G_dGCh'C
Cuts at: 0 190 233 275 788 855 993
Size: 190 43 42 513 67 138
Fragments arranged by size:
513 190 138 67 43 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 827 993
Size: 827 166
BsrBI CCG'CTC
Cuts at: 0 11 993
Size: 11 982
BsrDI GCAATG_nn'
Cuts at: 0 560 993
Size: 560 433
BsrFI r'CCGG_y
Cuts at: 0 740 993
Size: 740 253
BsrGI T'GTAC_A
Cuts at: 0 383 607 993
Size: 383 224 386
Fragments arranged by size:
386 383 224
BstAPI GCAn_nnn'nTGC
Cuts at: 0 374 936 993
Size: 374 562 57
Fragments arranged by size:
562 374 57
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 778 993
Size: 282 496 215
Fragments arranged by size:
496 282 215
BtsI GCAGTG_nn'
Cuts at: 0 764 993
Size: 764 229
DraIII CAC_nnn'GTG
Cuts at: 0 374 993
Size: 374 619
EaeI y'GGCC_r
Cuts at: 0 362 443 993
Size: 362 81 550
Fragments arranged by size:
550 362 81
EarI CTCTTCn'nnn_
Cuts at: 0 121 133 993
Size: 121 12 860
Fragments arranged by size:
860 121 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 523 993
Size: 523 470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 816 993
Size: 100 716 177
Fragments arranged by size:
716 177 100
EcoO109I rG'GnC_Cy
Cuts at: 0 851 852 993
Size: 851 1 141
Fragments arranged by size:
851 141 1
FspI TGC'GCA
Cuts at: 0 721 993
Size: 721 272
HincII GTy'rAC
Cuts at: 0 424 993
Size: 424 569
MscI TGG'CCA
Cuts at: 0 364 445 993
Size: 364 81 548
Fragments arranged by size:
548 364 81
MslI CAynn'nnrTG
Cuts at: 0 151 391 781 993
Size: 151 240 390 212
Fragments arranged by size:
390 240 212 151
MspA1I CmG'CkG
Cuts at: 0 712 747 993
Size: 712 35 246
Fragments arranged by size:
712 246 35
NaeI GCC'GGC
Cuts at: 0 742 993
Size: 742 251
NgoMIV G'CCGG_C
Cuts at: 0 740 993
Size: 740 253
NspI r_CATG'y
Cuts at: 0 934 993
Size: 934 59
PasI CC'CwG_GG
Cuts at: 0 735 993
Size: 735 258
PflMI CCAn_nnn'nTGG
Cuts at: 0 60 225 360 993
Size: 60 165 135 633
Fragments arranged by size:
633 165 135 60
PfoI T'CCnGG_A
Cuts at: 0 77 242 993
Size: 77 165 751
Fragments arranged by size:
751 165 77
PspOMI G'GGCC_C
Cuts at: 0 851 993
Size: 851 142
PstI C_TGCA'G
Cuts at: 0 666 824 993
Size: 666 158 169
Fragments arranged by size:
666 169 158
PvuII CAG'CTG
Cuts at: 0 712 993
Size: 712 281
SfcI C'TryA_G
Cuts at: 0 141 260 662 820 971 993
Size: 141 119 402 158 151 22
Fragments arranged by size:
402 158 151 141 119 22
SphI G_CATG'C
Cuts at: 0 934 993
Size: 934 59
StuI AGG'CCT
Cuts at: 0 290 993
Size: 290 703
StyI C'CwwG_G
Cuts at: 0 275 993
Size: 275 718
TatI w'GTAC_w
Cuts at: 0 383 607 791 993
Size: 383 224 184 202
Fragments arranged by size:
383 224 202 184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 57 993
Size: 57 936
Enzymes that do cut:
AccI AleI AlwNI ApaI AseI BaeI BanI BanII
BbsI BbvCI BcgI Bme1580I BmrI BpmI Bpu10I BsaAI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BstAPI BstXI BtsI DraIII EaeI EarI
EciI Eco57MI EcoO109I FspI HincII MscI MslI MspA1I
NaeI NgoMIV NspI PasI PflMI PfoI PspOMI PstI
PvuII SfcI SphI StuI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AsiSI
AvaI AvrII BamHI BbeI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspAI HaeII Hin4I
HindIII HpaI KasI KpnI MfeI MluI MmeI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI SwaI TaqII TstI Tth111I
XbaI XhoI XmaI XmnI ZraI