Restriction map for
pFN21AE5477
(Linear) (Six-base) MAPSORT of: KIEE5477.seq Check: 3735 from: 1 to: 987
KIEE5477.orf
With 182 enzymes: SgfI *
December 5, 2014 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 915 987
Size: 915 72
Acc65I G'GTAC_C
Cuts at: 0 735 987
Size: 735 252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 987
Size: 751 236
AflII C'TTAA_G
Cuts at: 0 757 987
Size: 757 230
AflIII A'CryG_T
Cuts at: 0 772 987
Size: 772 215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 149 987
Size: 149 838
ApoI r'AATT_y
Cuts at: 0 818 987
Size: 818 169
BanI G'GyrC_C
Cuts at: 0 735 987
Size: 735 252
BbsI GAAGACnn'nnnn_
Cuts at: 0 795 943 987
Size: 795 148 44
Fragments arranged by size:
795 148 44
BfrBI ATG'CAT
Cuts at: 0 370 987
Size: 370 617
BmtI G_CTAG'C
Cuts at: 0 253 987
Size: 253 734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 703 987
Size: 87 616 284
Fragments arranged by size:
616 284 87
Bpu10I CC'TnA_GC
Cuts at: 0 52 987
Size: 52 935
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 878 908 987
Size: 878 30 79
Fragments arranged by size:
878 79 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 267 366 886 987
Size: 267 99 520 101
Fragments arranged by size:
520 267 101 99
BseYI C'CCAG_C
Cuts at: 0 77 987
Size: 77 910
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 987
Size: 269 718
BsiHKAI G_wGCw'C
Cuts at: 0 308 965 987
Size: 308 657 22
Fragments arranged by size:
657 308 22
BsmI GAATG_Cn'
Cuts at: 0 370 987
Size: 370 617
Bsp1286I G_dGCh'C
Cuts at: 0 308 965 987
Size: 308 657 22
Fragments arranged by size:
657 308 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 915 987
Size: 915 72
BsrDI GCAATG_nn'
Cuts at: 0 183 987
Size: 183 804
BssSI C'ACGA_G
Cuts at: 0 322 987
Size: 322 665
BstXI CCAn_nnnn'nTGG
Cuts at: 0 264 328 411 540 987
Size: 264 64 83 129 447
Fragments arranged by size:
447 264 129 83 64
BstYI r'GATC_y
Cuts at: 0 947 987
Size: 947 40
BtgI C'CryG_G
Cuts at: 0 380 404 987
Size: 380 24 583
Fragments arranged by size:
583 380 24
BtsI GCAGTG_nn'
Cuts at: 0 136 281 308 731 987
Size: 136 145 27 423 256
Fragments arranged by size:
423 256 145 136 27
EaeI y'GGCC_r
Cuts at: 0 401 689 881 987
Size: 401 288 192 106
Fragments arranged by size:
401 288 192 106
EarI CTCTTCn'nnn_
Cuts at: 0 196 241 987
Size: 196 45 746
Fragments arranged by size:
746 196 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 940 987
Size: 940 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 703 751 987
Size: 87 616 48 236
Fragments arranged by size:
616 236 87 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 500 532 987
Size: 500 32 455
Fragments arranged by size:
500 455 32
KpnI G_GTAC'C
Cuts at: 0 739 987
Size: 739 248
MscI TGG'CCA
Cuts at: 0 403 691 883 987
Size: 403 288 192 104
Fragments arranged by size:
403 288 192 104
MslI CAynn'nnrTG
Cuts at: 0 202 487 788 869 987
Size: 202 285 301 81 118
Fragments arranged by size:
301 285 202 118 81
NcoI C'CATG_G
Cuts at: 0 380 404 987
Size: 380 24 583
Fragments arranged by size:
583 380 24
NdeI CA'TA_TG
Cuts at: 0 596 987
Size: 596 391
NheI G'CTAG_C
Cuts at: 0 249 987
Size: 249 738
NsiI A_TGCA'T
Cuts at: 0 372 987
Size: 372 615
NspI r_CATG'y
Cuts at: 0 776 987
Size: 776 211
PasI CC'CwG_GG
Cuts at: 0 144 987
Size: 144 843
PciI A'CATG_T
Cuts at: 0 772 987
Size: 772 215
SexAI A'CCwGG_T
Cuts at: 0 263 987
Size: 263 724
SmlI C'TyrA_G
Cuts at: 0 757 987
Size: 757 230
StyI C'CwwG_G
Cuts at: 0 380 404 987
Size: 380 24 583
Fragments arranged by size:
583 380 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 303 987
Size: 303 684
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 479 511 878 910 987
Size: 479 32 367 32 77
Fragments arranged by size:
479 367 77 32 32
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AlfI ApoI BanI
BbsI BfrBI BmtI BpmI Bpu10I BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BstXI
BstYI BtgI BtsI EaeI EarI EciI Eco57MI Hin4I
KpnI MscI MslI NcoI NdeI NheI NsiI NspI
PasI PciI SexAI SmlI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AhdI AleI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI MmeI MspA1I NaeI NarI NgoMIV NotI
NruI PacI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI