Restriction map for
pFN21AE5469
(Linear) (Six-base) MAPSORT of: KIEE5469.seq Check: 7129 from: 1 to: 954
KIEE5469.orf
With 182 enzymes: SgfI *
January 29, 2014 12:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 346 954
Size: 346 608
AccI GT'mk_AC
Cuts at: 0 158 425 954
Size: 158 267 529
Fragments arranged by size:
529 267 158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 224 475 954
Size: 3 221 251 479
Fragments arranged by size:
479 251 221 3
AlwNI CAG_nnn'CTG
Cuts at: 0 514 954
Size: 514 440
ApoI r'AATT_y
Cuts at: 0 100 398 954
Size: 100 298 556
Fragments arranged by size:
556 298 100
AseI AT'TA_AT
Cuts at: 0 221 954
Size: 221 733
BclI T'GATC_A
Cuts at: 0 212 954
Size: 212 742
BmrI ACTGGGnnnn_n'
Cuts at: 0 415 954
Size: 415 539
BmtI G_CTAG'C
Cuts at: 0 248 954
Size: 248 706
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 737 954
Size: 94 643 217
Fragments arranged by size:
643 217 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 954
Size: 812 142
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 954
Size: 109 845
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 140 170 954
Size: 140 30 784
Fragments arranged by size:
784 140 30
BseYI C'CCAG_C
Cuts at: 0 765 954
Size: 765 189
BspHI T'CATG_A
Cuts at: 0 215 954
Size: 215 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 127 346 954
Size: 127 219 608
Fragments arranged by size:
608 219 127
BsrDI GCAATG_nn'
Cuts at: 0 539 846 954
Size: 539 307 108
Fragments arranged by size:
539 307 108
BsrFI r'CCGG_y
Cuts at: 0 308 954
Size: 308 646
BstXI CCAn_nnnn'nTGG
Cuts at: 0 603 954
Size: 603 351
BtgI C'CryG_G
Cuts at: 0 377 954
Size: 377 577
BtsI GCAGTG_nn'
Cuts at: 0 785 809 954
Size: 785 24 145
Fragments arranged by size:
785 145 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 94 224 475 737 954
Size: 3 91 130 251 262 217
Fragments arranged by size:
262 251 217 130 91 3
EcoRV GAT'ATC
Cuts at: 0 417 954
Size: 417 537
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 140 172 954
Size: 140 32 782
Fragments arranged by size:
782 140 32
HindIII A'AGCT_T
Cuts at: 0 328 954
Size: 328 626
MslI CAynn'nnrTG
Cuts at: 0 376 877 954
Size: 376 501 77
Fragments arranged by size:
501 376 77
MspA1I CmG'CkG
Cuts at: 0 23 697 715 954
Size: 23 674 18 239
Fragments arranged by size:
674 239 23 18
NcoI C'CATG_G
Cuts at: 0 377 954
Size: 377 577
NheI G'CTAG_C
Cuts at: 0 244 954
Size: 244 710
NspI r_CATG'y
Cuts at: 0 391 794 954
Size: 391 403 160
Fragments arranged by size:
403 391 160
PflMI CCAn_nnn'nTGG
Cuts at: 0 514 954
Size: 514 440
PstI C_TGCA'G
Cuts at: 0 261 954
Size: 261 693
PvuII CAG'CTG
Cuts at: 0 697 715 954
Size: 697 18 239
Fragments arranged by size:
697 239 18
SfcI C'TryA_G
Cuts at: 0 257 350 954
Size: 257 93 604
Fragments arranged by size:
604 257 93
SmlI C'TyrA_G
Cuts at: 0 827 954
Size: 827 127
SphI G_CATG'C
Cuts at: 0 794 954
Size: 794 160
StyI C'CwwG_G
Cuts at: 0 377 954
Size: 377 577
XmnI GAAnn'nnTTC
Cuts at: 0 756 954
Size: 756 198
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI AseI BclI BmrI
BmtI BpmI BpuEI BsaI BsaXI BseYI BspHI BspMI
BsrDI BsrFI BstXI BtgI BtsI Eco57MI EcoRV Hin4I
HindIII MslI MspA1I NcoI NheI NspI PflMI PstI
PvuII SfcI SmlI SphI StyI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI