(Linear) (Six-base) MAPSORT of: KIEE5465.seq  Check: 2901  from: 1  to: 930



KIEE5465.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:01  ..

AflII C'TTAA_G

Cuts at:      0     483     930 
   Size:        483     447
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     222     930 
   Size:        222     708
 
ApoI r'AATT_y

Cuts at:      0      25     930 
   Size:         25     905
 
AseI AT'TA_AT

Cuts at:      0     235     930 
   Size:        235     695
 
BamHI G'GATC_C

Cuts at:      0      51     930 
   Size:         51     879
 
BclI T'GATC_A

Cuts at:      0     516     575     930 
   Size:        516      59     355

  Fragments arranged by size: 

                 516     355      59
 
BglII A'GATC_T

Cuts at:      0     326     930 
   Size:        326     604
 
BmgBI CAC'GTC

Cuts at:      0     716     930 
   Size:        716     214
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     600     930 
   Size:         78     522     330

  Fragments arranged by size: 

                 522     330      78
 
BsmI GAATG_Cn'

Cuts at:      0     660     870     930 
   Size:        660     210      60

  Fragments arranged by size: 

                 660     210      60
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     722     930 
   Size:        722     208
 
BspHI T'CATG_A

Cuts at:      0     398     626     930 
   Size:        398     228     304

  Fragments arranged by size: 

                 398     304     228
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     399     590     930 
   Size:        399     191     340

  Fragments arranged by size: 

                 399     340     191
 
BstYI r'GATC_y

Cuts at:      0      51     326     930 
   Size:         51     275     604

  Fragments arranged by size: 

                 604     275      51
 
BtgI C'CryG_G

Cuts at:      0     791     930 
   Size:        791     139
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     141     930 
   Size:        141     789
 
EaeI y'GGCC_r

Cuts at:      0     365     930 
   Size:        365     565
 
EcoRI G'AATT_C

Cuts at:      0      25     930 
   Size:         25     905
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     509     930 
   Size:        509     421
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     235     267     930 
   Size:        235      32     663

  Fragments arranged by size: 

                 663     235      32
 
MscI TGG'CCA

Cuts at:      0     367     930 
   Size:        367     563
 
MslI CAynn'nnrTG

Cuts at:      0     130     343     571     930 
   Size:        130     213     228     359

  Fragments arranged by size: 

                 359     228     213     130
 
NcoI C'CATG_G

Cuts at:      0     791     930 
   Size:        791     139
 
PshAI GACnn'nnGTC

Cuts at:      0     292     930 
   Size:        292     638
 
PsiI TTA'TAA

Cuts at:      0     268     930 
   Size:        268     662
 
SfcI C'TryA_G

Cuts at:      0     461     930 
   Size:        461     469
 
SmlI C'TyrA_G

Cuts at:      0      57     483     615     930 
   Size:         57     426     132     315

  Fragments arranged by size: 

                 426     315     132      57
 
StyI C'CwwG_G

Cuts at:      0     791     930 
   Size:        791     139
 
TatI w'GTAC_w

Cuts at:      0     170     930 
   Size:        170     760
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      95     127     930 
   Size:         95      32     803

  Fragments arranged by size: 

                 803      95      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     789     798     930 
   Size:        789       9     132

  Fragments arranged by size: 

                 789     132       9
 

 Enzymes that do cut:

    AflII     AlfI     ApoI     AseI    BamHI     BclI    BglII    BmgBI
    BpuEI     BsmI    BsmBI    BspHI    BspMI    BstYI     BtgI     DrdI
     EaeI    EcoRI     FalI    Hin4I     MscI     MslI     NcoI    PshAI
     PsiI     SfcI     SmlI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
 Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI