(Linear) (Six-base) MAPSORT of: KIEE5464.seq  Check: 735  from: 1  to: 927



KIEE5464.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:01  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     397     927 
   Size:        397     530
 
AlwNI CAG_nnn'CTG

Cuts at:      0     808     927 
   Size:        808     119
 
BclI T'GATC_A

Cuts at:      0     236     545     927 
   Size:        236     309     382

  Fragments arranged by size: 

                 382     309     236
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     884     927 
   Size:        884      43
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     761     927 
   Size:        761     166
 
Bpu10I CC'TnA_GC

Cuts at:      0     230     927 
   Size:        230     697
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     879     927 
   Size:        275     604      48

  Fragments arranged by size: 

                 604     275      48
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     645     765     927 
   Size:        645     120     162

  Fragments arranged by size: 

                 645     162     120
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     543     927 
   Size:        543     384
 
BsiHKAI G_wGCw'C

Cuts at:      0     125     927 
   Size:        125     802
 
BsmI GAATG_Cn'

Cuts at:      0     870     927 
   Size:        870      57
 
Bsp1286I G_dGCh'C

Cuts at:      0     125     927 
   Size:        125     802
 
BspHI T'CATG_A

Cuts at:      0     398     927 
   Size:        398     529
 
BsrDI GCAATG_nn'

Cuts at:      0     587     927 
   Size:        587     340
 
BsrGI T'GTAC_A

Cuts at:      0     764     927 
   Size:        764     163
 
BstEII G'GTnAC_C

Cuts at:      0     132     927 
   Size:        132     795
 
BstYI r'GATC_y

Cuts at:      0     160     470     927 
   Size:        160     310     457

  Fragments arranged by size: 

                 457     310     160
 
BtsI GCAGTG_nn'

Cuts at:      0     140     927 
   Size:        140     787
 
DraIII CAC_nnn'GTG

Cuts at:      0     363     927 
   Size:        363     564
 
EaeI y'GGCC_r

Cuts at:      0     365     927 
   Size:        365     562
 
EarI CTCTTCn'nnn_

Cuts at:      0     481     589     927 
   Size:        481     108     338

  Fragments arranged by size: 

                 481     338     108
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     761     927 
   Size:        761     166
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     285     927 
   Size:        285     642
 
MscI TGG'CCA

Cuts at:      0     367     927 
   Size:        367     560
 
MslI CAynn'nnrTG

Cuts at:      0     397     760     927 
   Size:        397     363     167

  Fragments arranged by size: 

                 397     363     167
 
NdeI CA'TA_TG

Cuts at:      0     610     927 
   Size:        610     317
 
NspI r_CATG'y

Cuts at:      0     412     927 
   Size:        412     515
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     363     927 
   Size:        363     564
 
PshAI GACnn'nnGTC

Cuts at:      0     292     927 
   Size:        292     635
 
SmlI C'TyrA_G

Cuts at:      0     290     858     927 
   Size:        290     568      69

  Fragments arranged by size: 

                 568     290      69
 
TatI w'GTAC_w

Cuts at:      0     170     659     764     927 
   Size:        170     489     105     163

  Fragments arranged by size: 

                 489     170     163     105
 

 Enzymes that do cut:

     AlfI    AlwNI     BclI     BplI     BpmI   Bpu10I    BpuEI     BsaI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI    BsrGI   BstEII
    BstYI     BtsI   DraIII     EaeI     EarI  Eco57MI     FalI     MscI
     MslI     NdeI     NspI    PflMI    PshAI     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI    BsmBI
    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI