(Linear) (Six-base) MAPSORT of: KIEE5463.seq  Check: 4616  from: 1  to: 927



KIEE5463.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:01  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     287     927 
   Size:        287     640
 
ApoI r'AATT_y

Cuts at:      0      60      90     137     927 
   Size:         60      30      47     790

  Fragments arranged by size: 

                 790      60      47      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     771     927 
   Size:        771     156
 
BbvCI CC'TCA_GC

Cuts at:      0     304     927 
   Size:        304     623
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     781     815     927 
   Size:        781      34     112

  Fragments arranged by size: 

                 781     112      34
 
BglII A'GATC_T

Cuts at:      0     119     927 
   Size:        119     808
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     906     927 
   Size:        906      21
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     927 
   Size:        144     783
 
Bpu10I CC'TnA_GC

Cuts at:      0     304     927 
   Size:        304     623
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     632     927 
   Size:        307     325     295

  Fragments arranged by size: 

                 325     307     295
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     381     519     927 
   Size:        381     138     408

  Fragments arranged by size: 

                 408     381     138
 
BseYI C'CCAG_C

Cuts at:      0     655     927 
   Size:        655     272
 
BsmI GAATG_Cn'

Cuts at:      0     802     927 
   Size:        802     125
 
BsrDI GCAATG_nn'

Cuts at:      0     518     927 
   Size:        518     409
 
BstEII G'GTnAC_C

Cuts at:      0     534     927 
   Size:        534     393
 
BstYI r'GATC_y

Cuts at:      0     119     643     651     927 
   Size:        119     524       8     276

  Fragments arranged by size: 

                 524     276     119       8
 
BtgI C'CryG_G

Cuts at:      0     633     927 
   Size:        633     294
 
BtsI GCAGTG_nn'

Cuts at:      0     143     927 
   Size:        143     784
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     927 
   Size:        144     783
 
EcoO109I rG'GnC_Cy

Cuts at:      0     403     927 
   Size:        403     524
 
EcoRV GAT'ATC

Cuts at:      0     578     927 
   Size:        578     349
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136     927 
   Size:        104      32     791

  Fragments arranged by size: 

                 791     104      32
 
HindIII A'AGCT_T

Cuts at:      0     677     927 
   Size:        677     250
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     927 
   Size:        365     562
 
MslI CAynn'nnrTG

Cuts at:      0     736     927 
   Size:        736     191
 
NcoI C'CATG_G

Cuts at:      0     633     927 
   Size:        633     294
 
NspI r_CATG'y

Cuts at:      0     889     927 
   Size:        889      38
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     101     927 
   Size:        101     826
 
SmlI C'TyrA_G

Cuts at:      0     322     647     927 
   Size:        322     325     280

  Fragments arranged by size: 

                 325     322     280
 
StyI C'CwwG_G

Cuts at:      0     633     927 
   Size:        633     294
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     839     888     927 
   Size:        839      49      39

  Fragments arranged by size: 

                 839      49      39
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      36     927 
   Size:         36     891
 

 Enzymes that do cut:

    AlwNI     ApoI     BbsI    BbvCI     BcgI    BglII     BmrI     BpmI
   Bpu10I    BpuEI    BseRI    BseYI     BsmI    BsrDI   BstEII    BstYI
     BtgI     BtsI  Eco57MI EcoO109I    EcoRV    Hin4I  HindIII     MmeI
     MslI     NcoI     NspI    PflMI     SmlI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI