(Linear) (Six-base) MAPSORT of: KIEE5458.seq  Check: 3607  from: 1  to: 951



KIEE5458.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:01  ..

AflIII A'CryG_T

Cuts at:      0     899     951 
   Size:        899      52
 
AleI CACnn'nnGTG

Cuts at:      0     830     951 
   Size:        830     121
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     649     951 
   Size:        649     302
 
ApoI r'AATT_y

Cuts at:      0      46      60     934     951 
   Size:         46      14     874      17

  Fragments arranged by size: 

                 874      46      17      14
 
BamHI G'GATC_C

Cuts at:      0     137     951 
   Size:        137     814
 
BanII G_rGCy'C

Cuts at:      0     920     951 
   Size:        920      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     408     951 
   Size:        408     543
 
BbvCI CC'TCA_GC

Cuts at:      0     892     951 
   Size:        892      59
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     466     951 
   Size:        466     485
 
BclI T'GATC_A

Cuts at:      0     272     698     951 
   Size:        272     426     253

  Fragments arranged by size: 

                 426     272     253
 
BglII A'GATC_T

Cuts at:      0     119     951 
   Size:        119     832
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     111     951 
   Size:        111     840
 
Bpu10I CC'TnA_GC

Cuts at:      0     892     951 
   Size:        892      59
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     951 
   Size:        310     641
 
BsaBI GATnn'nnATC

Cuts at:      0     445     951 
   Size:        445     506
 
BsaHI Gr'CG_yC

Cuts at:      0     662     951 
   Size:        662     289
 
BsaWI w'CCGG_w

Cuts at:      0     655     951 
   Size:        655     296
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     589     698     951 
   Size:        589     109     253

  Fragments arranged by size: 

                 589     253     109
 
BseYI C'CCAG_C

Cuts at:      0     216     951 
   Size:        216     735
 
BsmI GAATG_Cn'

Cuts at:      0     254     951 
   Size:        254     697
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     653     951 
   Size:        653     298
 
Bsp1286I G_dGCh'C

Cuts at:      0     920     951 
   Size:        920      31
 
BspEI T'CCGG_A

Cuts at:      0     655     951 
   Size:        655     296
 
BsrDI GCAATG_nn'

Cuts at:      0     593     951 
   Size:        593     358
 
BstYI r'GATC_y

Cuts at:      0     119     137     951 
   Size:        119      18     814

  Fragments arranged by size: 

                 814     119      18
 
Bsu36I CC'TnA_GG

Cuts at:      0     921     951 
   Size:        921      30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     607     951 
   Size:        607     344
 
BtsI GCAGTG_nn'

Cuts at:      0     143     951 
   Size:        143     808
 
DraI TTT'AAA

Cuts at:      0     428     951 
   Size:        428     523
 
EarI CTCTTCn'nnn_

Cuts at:      0     129     951 
   Size:        129     822
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     762     951 
   Size:        762     189
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     749     951 
   Size:        749     202
 
MslI CAynn'nnrTG

Cuts at:      0     284     455     830     951 
   Size:        284     171     375     121

  Fragments arranged by size: 

                 375     284     171     121
 
NspI r_CATG'y

Cuts at:      0     903     951 
   Size:        903      48
 
PciI A'CATG_T

Cuts at:      0     899     951 
   Size:        899      52
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     801     951 
   Size:        801     150
 
PshAI GACnn'nnGTC

Cuts at:      0     412     951 
   Size:        412     539
 
PsiI TTA'TAA

Cuts at:      0      52     951 
   Size:         52     899
 
SfcI C'TryA_G

Cuts at:      0     299     951 
   Size:        299     652
 
SmlI C'TyrA_G

Cuts at:      0     325     951 
   Size:        325     626
 
SspI AAT'ATT

Cuts at:      0     185     432     951 
   Size:        185     247     519

  Fragments arranged by size: 

                 519     247     185
 
StuI AGG'CCT

Cuts at:      0     305     868     951 
   Size:        305     563      83

  Fragments arranged by size: 

                 563     305      83
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     402     951 
   Size:        402     549
 
Tth111I GACn'n_nGTC

Cuts at:      0     103     951 
   Size:        103     848
 

 Enzymes that do cut:

   AflIII     AleI     AlfI     ApoI    BamHI    BanII     BbsI    BbvCI
    BciVI     BclI    BglII     BmrI   Bpu10I    BpuEI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsmI    BsmBI Bsp1286I    BspEI    BsrDI
    BstYI   Bsu36I    BtgZI     BtsI     DraI     EarI    EcoNI     MmeI
     MslI     NspI     PciI    PflMI    PshAI     PsiI     SfcI     SmlI
     SspI     StuI    TaqII  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BcgI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI     BsaI
    BsaAI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StyI     SwaI
     TatI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI