Restriction map for
pFN21AE5455
(Linear) (Six-base) MAPSORT of: KIEE5455.seq Check: 5280 from: 1 to: 930
KIEE5455.orf
With 182 enzymes: SgfI *
January 29, 2014 12:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 724 930
Size: 724 206
AccI GT'mk_AC
Cuts at: 0 203 930
Size: 203 727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 930
Size: 658 272
AleI CACnn'nnGTG
Cuts at: 0 731 930
Size: 731 199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 244 930
Size: 244 686
ApoI r'AATT_y
Cuts at: 0 515 930
Size: 515 415
BanI G'GyrC_C
Cuts at: 0 228 930
Size: 228 702
BanII G_rGCy'C
Cuts at: 0 86 140 930
Size: 86 54 790
Fragments arranged by size:
790 86 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 96 930
Size: 96 834
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 408 442 930
Size: 408 34 488
Fragments arranged by size:
488 408 34
Bme1580I G_kGCm'C
Cuts at: 0 233 930
Size: 233 697
BmrI ACTGGGnnnn_n'
Cuts at: 0 49 142 930
Size: 49 93 788
Fragments arranged by size:
788 93 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 49 930
Size: 49 881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 429 470 930
Size: 138 291 41 460
Fragments arranged by size:
460 291 138 41
BsaAI yAC'GTr
Cuts at: 0 366 930
Size: 366 564
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 325 930
Size: 64 261 605
Fragments arranged by size:
605 261 64
BseYI C'CCAG_C
Cuts at: 0 114 909 930
Size: 114 795 21
Fragments arranged by size:
795 114 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 584 930
Size: 145 439 346
Fragments arranged by size:
439 346 145
BsiEI CG_ry'CG
Cuts at: 0 200 930
Size: 200 730
Bsp1286I G_dGCh'C
Cuts at: 0 86 140 233 930
Size: 86 54 93 697
Fragments arranged by size:
697 93 86 54
BspHI T'CATG_A
Cuts at: 0 401 930
Size: 401 529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 724 930
Size: 724 206
BstYI r'GATC_y
Cuts at: 0 677 930
Size: 677 253
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 192 930
Size: 192 738
EaeI y'GGCC_r
Cuts at: 0 368 395 596 930
Size: 368 27 201 334
Fragments arranged by size:
368 334 201 27
EarI CTCTTCn'nnn_
Cuts at: 0 91 895 930
Size: 91 804 35
Fragments arranged by size:
804 91 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 429 470 658 930
Size: 138 291 41 188 272
Fragments arranged by size:
291 272 188 138 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 501 930
Size: 501 429
EcoO109I rG'GnC_Cy
Cuts at: 0 506 622 930
Size: 506 116 308
Fragments arranged by size:
506 308 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 404 436 930
Size: 404 32 494
Fragments arranged by size:
494 404 32
HincII GTy'rAC
Cuts at: 0 204 930
Size: 204 726
MfeI C'AATT_G
Cuts at: 0 635 930
Size: 635 295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 756 930
Size: 756 174
MscI TGG'CCA
Cuts at: 0 370 397 930
Size: 370 27 533
Fragments arranged by size:
533 370 27
MslI CAynn'nnrTG
Cuts at: 0 578 651 731 766 930
Size: 578 73 80 35 164
Fragments arranged by size:
578 164 80 73 35
PfoI T'CCnGG_A
Cuts at: 0 439 930
Size: 439 491
PpuMI rG'GwC_Cy
Cuts at: 0 622 930
Size: 622 308
PshAI GACnn'nnGTC
Cuts at: 0 627 930
Size: 627 303
SalI G'TCGA_C
Cuts at: 0 202 930
Size: 202 728
SanDI GG'GwC_CC
Cuts at: 0 622 930
Size: 622 308
SapI GCTCTTCn'nnn_
Cuts at: 0 91 930
Size: 91 839
SfcI C'TryA_G
Cuts at: 0 501 864 930
Size: 501 363 66
Fragments arranged by size:
501 363 66
StyI C'CwwG_G
Cuts at: 0 58 797 930
Size: 58 739 133
Fragments arranged by size:
739 133 58
TatI w'GTAC_w
Cuts at: 0 173 930
Size: 173 757
XmnI GAAnn'nnTTC
Cuts at: 0 706 930
Size: 706 224
Enzymes that do cut:
AarI AccI AcuI AleI AlfI ApoI BanI BanII
BbsI BcgI Bme1580I BmrI BplI BpmI BsaAI BseRI
BseYI BsgI BsiEI Bsp1286I BspHI BspMI BstYI BtgZI
EaeI EarI Eco57MI EcoNI EcoO109I Hin4I HincII MfeI
MmeI MscI MslI PfoI PpuMI PshAI SalI SanDI
SapI SfcI StyI TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BsiHKAI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MluI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI