(Linear) (Six-base) MAPSORT of: KIEE5450.seq  Check: 5679  from: 1  to: 1011



KIEE5450.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 12:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     524     634    1011 
   Size:         98     426     110     377

  Fragments arranged by size: 

                 426     377     110      98
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     247    1011 
   Size:        247     764
 
ApoI r'AATT_y

Cuts at:      0     152    1011 
   Size:        152     859
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     324     638    1011 
   Size:        324     314     373

  Fragments arranged by size: 

                 373     324     314
 
BlpI GC'TnA_GC

Cuts at:      0     200    1011 
   Size:        200     811
 
BmtI G_CTAG'C

Cuts at:      0     208    1011 
   Size:        208     803
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     333    1011 
   Size:        333     678
 
BsaAI yAC'GTr

Cuts at:      0     513    1011 
   Size:        513     498
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     865     895    1011 
   Size:        865      30     116

  Fragments arranged by size: 

                 865     116      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     376    1011 
   Size:        376     635
 
BsiHKAI G_wGCw'C

Cuts at:      0     363    1011 
   Size:        363     648
 
Bsp1286I G_dGCh'C

Cuts at:      0     363    1011 
   Size:        363     648
 
BspHI T'CATG_A

Cuts at:      0     731     770    1011 
   Size:        731      39     241

  Fragments arranged by size: 

                 731     241      39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     263    1011 
   Size:        263     748
 
BsrDI GCAATG_nn'

Cuts at:      0     149    1011 
   Size:        149     862
 
BstYI r'GATC_y

Cuts at:      0     875     983    1011 
   Size:        875     108      28

  Fragments arranged by size: 

                 875     108      28
 
BtsI GCAGTG_nn'

Cuts at:      0     133     200     231    1011 
   Size:        133      67      31     780

  Fragments arranged by size: 

                 780     133      67      31
 
DraI TTT'AAA

Cuts at:      0     686    1011 
   Size:        686     325
 
EaeI y'GGCC_r

Cuts at:      0      74     842    1011 
   Size:         74     768     169

  Fragments arranged by size: 

                 768     169      74
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     524     634    1011 
   Size:         98     426     110     377

  Fragments arranged by size: 

                 426     377     110      98
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     987    1011 
   Size:        987      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     315     347    1011 
   Size:        315      32     664

  Fragments arranged by size: 

                 664     315      32
 
HincII GTy'rAC

Cuts at:      0     524    1011 
   Size:        524     487
 
MscI TGG'CCA

Cuts at:      0      76     844    1011 
   Size:         76     768     167

  Fragments arranged by size: 

                 768     167      76
 
MspA1I CmG'CkG

Cuts at:      0     261    1011 
   Size:        261     750
 
NheI G'CTAG_C

Cuts at:      0     204    1011 
   Size:        204     807
 
PfoI T'CCnGG_A

Cuts at:      0     707    1011 
   Size:        707     304
 
PstI C_TGCA'G

Cuts at:      0     260    1011 
   Size:        260     751
 
PvuII CAG'CTG

Cuts at:      0     261    1011 
   Size:        261     750
 
SfcI C'TryA_G

Cuts at:      0     219     256    1011 
   Size:        219      37     755

  Fragments arranged by size: 

                 755     219      37
 
SmlI C'TyrA_G

Cuts at:      0     312    1011 
   Size:        312     699
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     321     353    1011 
   Size:        321      32     658

  Fragments arranged by size: 

                 658     321      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     446     818    1011 
   Size:        446     372     193

  Fragments arranged by size: 

                 446     372     193
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     BbsI     BlpI     BmtI    BpuEI    BsaAI
    BsaXI    BseRI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BstYI
     BtsI     DraI     EaeI  Eco57MI     FalI    Hin4I   HincII     MscI
   MspA1I     NheI     PfoI     PstI    PvuII     SfcI     SmlI     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaBI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI