Restriction map for
pFN21AE5449
(Linear) (Six-base) MAPSORT of: KIEE5449.seq Check: 9164 from: 1 to: 1005
KIEE5449.orf
With 182 enzymes: SgfI *
January 29, 2014 12:01 ..
AccI GT'mk_AC
Cuts at: 0 301 1005
Size: 301 704
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1005
Size: 968 37
AfeI AGC'GCT
Cuts at: 0 28 1005
Size: 28 977
AflII C'TTAA_G
Cuts at: 0 30 1005
Size: 30 975
AlwNI CAG_nnn'CTG
Cuts at: 0 946 1005
Size: 946 59
ApoI r'AATT_y
Cuts at: 0 39 1005
Size: 39 966
AseI AT'TA_AT
Cuts at: 0 770 1005
Size: 770 235
BamHI G'GATC_C
Cuts at: 0 158 1005
Size: 158 847
BanI G'GyrC_C
Cuts at: 0 681 928 1005
Size: 681 247 77
Fragments arranged by size:
681 247 77
BanII G_rGCy'C
Cuts at: 0 10 156 1005
Size: 10 146 849
Fragments arranged by size:
849 146 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 191 956 1005
Size: 191 765 49
Fragments arranged by size:
765 191 49
BbvCI CC'TCA_GC
Cuts at: 0 939 1005
Size: 939 66
BclI T'GATC_A
Cuts at: 0 363 1005
Size: 363 642
BglII A'GATC_T
Cuts at: 0 579 1005
Size: 579 426
BlpI GC'TnA_GC
Cuts at: 0 23 1005
Size: 23 982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 313 1005
Size: 235 78 692
Fragments arranged by size:
692 235 78
Bpu10I CC'TnA_GC
Cuts at: 0 939 1005
Size: 939 66
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 311 551 647 1005
Size: 311 240 96 358
Fragments arranged by size:
358 311 240 96
BseYI C'CCAG_C
Cuts at: 0 3 136 1005
Size: 3 133 869
Fragments arranged by size:
869 133 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 1005
Size: 779 226
BsiHKAI G_wGCw'C
Cuts at: 0 156 173 1005
Size: 156 17 832
Fragments arranged by size:
832 156 17
Bsp1286I G_dGCh'C
Cuts at: 0 10 156 173 1005
Size: 10 146 17 832
Fragments arranged by size:
832 146 17 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 861 1005
Size: 239 622 144
Fragments arranged by size:
622 239 144
BssSI C'ACGA_G
Cuts at: 0 193 1005
Size: 193 812
BstXI CCAn_nnnn'nTGG
Cuts at: 0 480 1005
Size: 480 525
BstYI r'GATC_y
Cuts at: 0 158 209 579 1005
Size: 158 51 370 426
Fragments arranged by size:
426 370 158 51
BtgI C'CryG_G
Cuts at: 0 203 1005
Size: 203 802
BtsI GCAGTG_nn'
Cuts at: 0 742 1005
Size: 742 263
DraI TTT'AAA
Cuts at: 0 18 1005
Size: 18 987
DrdI GACnn_nn'nnGTC
Cuts at: 0 544 874 1005
Size: 544 330 131
Fragments arranged by size:
544 330 131
EarI CTCTTCn'nnn_
Cuts at: 0 624 795 980 1005
Size: 624 171 185 25
Fragments arranged by size:
624 185 171 25
EcoICRI GAG'CTC
Cuts at: 0 154 1005
Size: 154 851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 313 968 1005
Size: 235 78 655 37
Fragments arranged by size:
655 235 78 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 196 629 1005
Size: 196 433 376
Fragments arranged by size:
433 376 196
EcoO109I rG'GnC_Cy
Cuts at: 0 622 1005
Size: 622 383
HaeII r_GCGC'y
Cuts at: 0 30 1005
Size: 30 975
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 143 175 792 824 877 909 1005
Size: 143 32 617 32 53 32 96
Fragments arranged by size:
617 143 96 53 32 32 32
HindIII A'AGCT_T
Cuts at: 0 838 1005
Size: 838 167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 1005
Size: 573 432
MslI CAynn'nnrTG
Cuts at: 0 697 703 709 1005
Size: 697 6 6 296
Fragments arranged by size:
697 296 6 6
NcoI C'CATG_G
Cuts at: 0 203 1005
Size: 203 802
PflMI CCAn_nnn'nTGG
Cuts at: 0 664 1005
Size: 664 341
PfoI T'CCnGG_A
Cuts at: 0 179 212 810 973 1005
Size: 179 33 598 163 32
Fragments arranged by size:
598 179 163 33 32
PshAI GACnn'nnGTC
Cuts at: 0 330 1005
Size: 330 675
PstI C_TGCA'G
Cuts at: 0 858 1005
Size: 858 147
SacI G_AGCT'C
Cuts at: 0 156 1005
Size: 156 849
SapI GCTCTTCn'nnn_
Cuts at: 0 980 1005
Size: 980 25
SfcI C'TryA_G
Cuts at: 0 854 1005
Size: 854 151
SmlI C'TyrA_G
Cuts at: 0 30 1005
Size: 30 975
StyI C'CwwG_G
Cuts at: 0 203 1005
Size: 203 802
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 486 1005
Size: 486 519
TatI w'GTAC_w
Cuts at: 0 221 1005
Size: 221 784
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 461 493 1005
Size: 461 32 512
Fragments arranged by size:
512 461 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 210 480 1005
Size: 210 270 525
Fragments arranged by size:
525 270 210
Enzymes that do cut:
AccI AcuI AfeI AflII AlwNI ApoI AseI BamHI
BanI BanII BbsI BbvCI BclI BglII BlpI BpmI
Bpu10I BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BssSI
BstXI BstYI BtgI BtsI DraI DrdI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MmeI MslI
NcoI PflMI PfoI PshAI PstI SacI SapI SfcI
SmlI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BfrBI BglI Bme1580I BmgBI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EaeI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI