Restriction map for
pFN21AE5444
(Linear) (Six-base) MAPSORT of: KIEE5444.seq Check: 5025 from: 1 to: 936
KIEE5444.orf
With 182 enzymes: SgfI *
January 29, 2014 12:00 ..
AccI GT'mk_AC
Cuts at: 0 384 936
Size: 384 552
Acc65I G'GTAC_C
Cuts at: 0 498 936
Size: 498 438
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 936
Size: 688 248
AflIII A'CryG_T
Cuts at: 0 721 936
Size: 721 215
AlwNI CAG_nnn'CTG
Cuts at: 0 246 936
Size: 246 690
ApaI G_GGCC'C
Cuts at: 0 265 936
Size: 265 671
ApoI r'AATT_y
Cuts at: 0 798 936
Size: 798 138
AvaI C'yCGr_G
Cuts at: 0 668 936
Size: 668 268
BanI G'GyrC_C
Cuts at: 0 498 658 936
Size: 498 160 278
Fragments arranged by size:
498 278 160
BanII G_rGCy'C
Cuts at: 0 265 613 732 936
Size: 265 348 119 204
Fragments arranged by size:
348 265 204 119
BbsI GAAGACnn'nnnn_
Cuts at: 0 35 936
Size: 35 901
BbvCI CC'TCA_GC
Cuts at: 0 65 936
Size: 65 871
BclI T'GATC_A
Cuts at: 0 807 936
Size: 807 129
Bme1580I G_kGCm'C
Cuts at: 0 265 936
Size: 265 671
BmrI ACTGGGnnnn_n'
Cuts at: 0 331 707 936
Size: 331 376 229
Fragments arranged by size:
376 331 229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 936
Size: 766 170
Bpu10I CC'TnA_GC
Cuts at: 0 65 936
Size: 65 871
BsaAI yAC'GTr
Cuts at: 0 153 936
Size: 153 783
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 142 172 936
Size: 142 30 764
Fragments arranged by size:
764 142 30
BsiHKAI G_wGCw'C
Cuts at: 0 613 936
Size: 613 323
Bsp1286I G_dGCh'C
Cuts at: 0 265 613 732 936
Size: 265 348 119 204
Fragments arranged by size:
348 265 204 119
BspHI T'CATG_A
Cuts at: 0 605 936
Size: 605 331
BsrDI GCAATG_nn'
Cuts at: 0 662 821 936
Size: 662 159 115
Fragments arranged by size:
662 159 115
BstYI r'GATC_y
Cuts at: 0 210 936
Size: 210 726
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 499 936
Size: 499 437
EarI CTCTTCn'nnn_
Cuts at: 0 901 936
Size: 901 35
EcoICRI GAG'CTC
Cuts at: 0 611 936
Size: 611 325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 766 936
Size: 688 78 170
Fragments arranged by size:
688 170 78
EcoO109I rG'GnC_Cy
Cuts at: 0 261 936
Size: 261 675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 843 875 936
Size: 843 32 61
Fragments arranged by size:
843 61 32
HincII GTy'rAC
Cuts at: 0 573 936
Size: 573 363
KpnI G_GTAC'C
Cuts at: 0 502 936
Size: 502 434
MslI CAynn'nnrTG
Cuts at: 0 175 584 604 772 936
Size: 175 409 20 168 164
Fragments arranged by size:
409 175 168 164 20
MspA1I CmG'CkG
Cuts at: 0 69 936
Size: 69 867
NdeI CA'TA_TG
Cuts at: 0 360 936
Size: 360 576
NspI r_CATG'y
Cuts at: 0 687 725 777 892 936
Size: 687 38 52 115 44
Fragments arranged by size:
687 115 52 44 38
PciI A'CATG_T
Cuts at: 0 721 936
Size: 721 215
PflMI CCAn_nnn'nTGG
Cuts at: 0 432 738 936
Size: 432 306 198
Fragments arranged by size:
432 306 198
PspOMI G'GGCC_C
Cuts at: 0 261 936
Size: 261 675
PvuII CAG'CTG
Cuts at: 0 69 936
Size: 69 867
SacI G_AGCT'C
Cuts at: 0 613 936
Size: 613 323
SmaI CCC'GGG
Cuts at: 0 670 936
Size: 670 266
SphI G_CATG'C
Cuts at: 0 687 936
Size: 687 249
SrfI GCCC'GGGC
Cuts at: 0 670 936
Size: 670 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 592 936
Size: 592 344
TatI w'GTAC_w
Cuts at: 0 179 675 936
Size: 179 496 261
Fragments arranged by size:
496 261 179
XmaI C'CCGG_G
Cuts at: 0 668 936
Size: 668 268
XmnI GAAnn'nnTTC
Cuts at: 0 77 936
Size: 77 859
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlwNI ApaI ApoI AvaI
BanI BanII BbsI BbvCI BclI Bme1580I BmrI BpmI
Bpu10I BsaAI BsaXI BsiHKAI Bsp1286I BspHI BsrDI BstYI
BtgZI EarI EcoICRI Eco57MI EcoO109I Hin4I HincII KpnI
MslI MspA1I NdeI NspI PciI PflMI PspOMI PvuII
SacI SmaI SphI SrfI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI MfeI MluI MmeI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SspI StuI StyI SwaI TstI Tth111I
XbaI XcmI XhoI ZraI