Restriction map for
pFN21AE5431
(Linear) (Six-base) MAPSORT of: KIEE5431.seq Check: 7550 from: 1 to: 1044
KIEE5431.orf
With 182 enzymes: SgfI *
January 29, 2014 12:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 739 1044
Size: 638 101 305
Fragments arranged by size:
638 305 101
AflIII A'CryG_T
Cuts at: 0 287 1044
Size: 287 757
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 274 306 1044
Size: 274 32 738
Fragments arranged by size:
738 274 32
AlwNI CAG_nnn'CTG
Cuts at: 0 270 485 582 1044
Size: 270 215 97 462
Fragments arranged by size:
462 270 215 97
ApaI G_GGCC'C
Cuts at: 0 7 1044
Size: 7 1037
ApoI r'AATT_y
Cuts at: 0 233 439 968 1044
Size: 233 206 529 76
Fragments arranged by size:
529 233 206 76
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 375 408 1044
Size: 375 33 636
Fragments arranged by size:
636 375 33
BanI G'GyrC_C
Cuts at: 0 106 907 1044
Size: 106 801 137
Fragments arranged by size:
801 137 106
BanII G_rGCy'C
Cuts at: 0 7 209 896 1044
Size: 7 202 687 148
Fragments arranged by size:
687 202 148 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 389 1044
Size: 389 655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 502 536 1044
Size: 502 34 508
Fragments arranged by size:
508 502 34
BclI T'GATC_A
Cuts at: 0 183 1044
Size: 183 861
BglI GCCn_nnn'nGGC
Cuts at: 0 19 1044
Size: 19 1025
Bme1580I G_kGCm'C
Cuts at: 0 7 1022 1044
Size: 7 1015 22
Fragments arranged by size:
1015 22 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 568 604 1044
Size: 520 48 36 440
Fragments arranged by size:
520 440 48 36
BsaAI yAC'GTr
Cuts at: 0 156 842 1044
Size: 156 686 202
Fragments arranged by size:
686 202 156
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 1022 1044
Size: 19 1003 22
Fragments arranged by size:
1003 22 19
BseYI C'CCAG_C
Cuts at: 0 588 1044
Size: 588 456
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 548 1044
Size: 73 475 496
Fragments arranged by size:
496 475 73
BsiHKAI G_wGCw'C
Cuts at: 0 857 1044
Size: 857 187
Bsp1286I G_dGCh'C
Cuts at: 0 7 209 857 896 1022 1044
Size: 7 202 648 39 126 22
Fragments arranged by size:
648 202 126 39 22 7
BspHI T'CATG_A
Cuts at: 0 186 1044
Size: 186 858
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 345 1044
Size: 345 699
BsrFI r'CCGG_y
Cuts at: 0 18 1044
Size: 18 1026
BstAPI GCAn_nnn'nTGC
Cuts at: 0 579 1044
Size: 579 465
BstBI TT'CG_AA
Cuts at: 0 752 1044
Size: 752 292
BstXI CCAn_nnnn'nTGG
Cuts at: 0 498 1044
Size: 498 546
BtgI C'CryG_G
Cuts at: 0 50 217 1013 1044
Size: 50 167 796 31
Fragments arranged by size:
796 167 50 31
EarI CTCTTCn'nnn_
Cuts at: 0 103 657 821 985 1044
Size: 103 554 164 164 59
Fragments arranged by size:
554 164 164 103 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 568 604 638 739 1044
Size: 520 48 36 34 101 305
Fragments arranged by size:
520 305 101 48 36 34
EcoO109I rG'GnC_Cy
Cuts at: 0 120 482 539 785 1044
Size: 120 362 57 246 259
Fragments arranged by size:
362 259 246 120 57
EcoRI G'AATT_C
Cuts at: 0 439 1044
Size: 439 605
HaeII r_GCGC'y
Cuts at: 0 25 69 1044
Size: 25 44 975
Fragments arranged by size:
975 44 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 252 284 1044
Size: 252 32 760
Fragments arranged by size:
760 252 32
HincII GTy'rAC
Cuts at: 0 300 1044
Size: 300 744
HindIII A'AGCT_T
Cuts at: 0 143 1044
Size: 143 901
MslI CAynn'nnrTG
Cuts at: 0 706 1044
Size: 706 338
MspA1I CmG'CkG
Cuts at: 0 52 168 258 570 1044
Size: 52 116 90 312 474
Fragments arranged by size:
474 312 116 90 52
NaeI GCC'GGC
Cuts at: 0 20 1044
Size: 20 1024
NcoI C'CATG_G
Cuts at: 0 217 1013 1044
Size: 217 796 31
Fragments arranged by size:
796 217 31
NgoMIV G'CCGG_C
Cuts at: 0 18 1044
Size: 18 1026
NspI r_CATG'y
Cuts at: 0 291 1044
Size: 291 753
PasI CC'CwG_GG
Cuts at: 0 479 820 1044
Size: 479 341 224
Fragments arranged by size:
479 341 224
PciI A'CATG_T
Cuts at: 0 287 1044
Size: 287 757
PflMI CCAn_nnn'nTGG
Cuts at: 0 485 713 1044
Size: 485 228 331
Fragments arranged by size:
485 331 228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 984 1016 1044
Size: 984 32 28
Fragments arranged by size:
984 32 28
PpuMI rG'GwC_Cy
Cuts at: 0 120 785 1044
Size: 120 665 259
Fragments arranged by size:
665 259 120
PshAI GACnn'nnGTC
Cuts at: 0 52 125 1044
Size: 52 73 919
Fragments arranged by size:
919 73 52
PspOMI G'GGCC_C
Cuts at: 0 3 1044
Size: 3 1041
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 526 558 1044
Size: 526 32 486
Fragments arranged by size:
526 486 32
PstI C_TGCA'G
Cuts at: 0 62 257 356 575 1044
Size: 62 195 99 219 469
Fragments arranged by size:
469 219 195 99 62
PvuII CAG'CTG
Cuts at: 0 168 258 570 1044
Size: 168 90 312 474
Fragments arranged by size:
474 312 168 90
SacII CC_GC'GG
Cuts at: 0 53 1044
Size: 53 991
SapI GCTCTTCn'nnn_
Cuts at: 0 821 1044
Size: 821 223
SbfI CC_TGCA'GG
Cuts at: 0 62 356 1044
Size: 62 294 688
Fragments arranged by size:
688 294 62
SfcI C'TryA_G
Cuts at: 0 58 253 352 571 1044
Size: 58 195 99 219 473
Fragments arranged by size:
473 219 195 99 58
SspI AAT'ATT
Cuts at: 0 983 1044
Size: 983 61
StuI AGG'CCT
Cuts at: 0 863 1044
Size: 863 181
StyI C'CwwG_G
Cuts at: 0 123 217 743 806 1013 1044
Size: 123 94 526 63 207 31
Fragments arranged by size:
526 207 123 94 63 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 94 872 1044
Size: 94 778 172
Fragments arranged by size:
778 172 94
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApaI ApoI BaeI BanI
BanII BbsI BcgI BclI BglI Bme1580I BpmI BsaAI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrFI
BstAPI BstBI BstXI BtgI EarI Eco57MI EcoO109I EcoRI
HaeII Hin4I HincII HindIII MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PciI PflMI PpiI PpuMI PshAI
PspOMI PsrI PstI PvuII SacII SapI SbfI SfcI
SspI StuI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BbvCI BciVI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrGI BssHII BssSI BstEII BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI HpaI KasI KpnI MfeI MluI MmeI
MscI NarI NdeI NheI NotI NruI NsiI PacI
PfoI PmeI PmlI PsiI PspXI PvuI RsrII SacI
SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI