Restriction map for
pFN21AE5430
(Linear) (Six-base) MAPSORT of: KIEE5430.seq Check: 4294 from: 1 to: 957
KIEE5430.orf
With 182 enzymes: SgfI *
January 29, 2014 12:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 730 957
Size: 730 227
AleI CACnn'nnGTG
Cuts at: 0 742 957
Size: 742 215
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 617 649 957
Size: 617 32 308
Fragments arranged by size:
617 308 32
AlwNI CAG_nnn'CTG
Cuts at: 0 446 957
Size: 446 511
AvrII C'CTAG_G
Cuts at: 0 189 621 957
Size: 189 432 336
Fragments arranged by size:
432 336 189
BamHI G'GATC_C
Cuts at: 0 683 957
Size: 683 274
BbsI GAAGACnn'nnnn_
Cuts at: 0 651 705 744 957
Size: 651 54 39 213
Fragments arranged by size:
651 213 54 39
BbvCI CC'TCA_GC
Cuts at: 0 690 695 957
Size: 690 5 262
Fragments arranged by size:
690 262 5
BclI T'GATC_A
Cuts at: 0 404 590 957
Size: 404 186 367
Fragments arranged by size:
404 367 186
Bpu10I CC'TnA_GC
Cuts at: 0 346 690 695 957
Size: 346 344 5 262
Fragments arranged by size:
346 344 262 5
BsaI GGTCTCn'nnnn_
Cuts at: 0 10 930 957
Size: 10 920 27
Fragments arranged by size:
920 27 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 503 533 617 647 957
Size: 503 30 84 30 310
Fragments arranged by size:
503 310 84 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 219 939 957
Size: 219 720 18
Fragments arranged by size:
720 219 18
BseYI C'CCAG_C
Cuts at: 0 120 957
Size: 120 837
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 319 957
Size: 151 168 638
Fragments arranged by size:
638 168 151
BsiHKAI G_wGCw'C
Cuts at: 0 92 957
Size: 92 865
Bsp1286I G_dGCh'C
Cuts at: 0 92 957
Size: 92 865
BspHI T'CATG_A
Cuts at: 0 407 957
Size: 407 550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 730 957
Size: 730 227
BsrDI GCAATG_nn'
Cuts at: 0 132 957
Size: 132 825
BssSI C'ACGA_G
Cuts at: 0 109 957
Size: 109 848
BstEII G'GTnAC_C
Cuts at: 0 144 861 957
Size: 144 717 96
Fragments arranged by size:
717 144 96
BstYI r'GATC_y
Cuts at: 0 683 822 957
Size: 683 139 135
Fragments arranged by size:
683 139 135
Bsu36I CC'TnA_GG
Cuts at: 0 46 391 679 895 957
Size: 46 345 288 216 62
Fragments arranged by size:
345 288 216 62 46
BtgI C'CryG_G
Cuts at: 0 320 431 470 740 803 957
Size: 320 111 39 270 63 154
Fragments arranged by size:
320 270 154 111 63 39
EaeI y'GGCC_r
Cuts at: 0 374 957
Size: 374 583
EcoO109I rG'GnC_Cy
Cuts at: 0 132 761 957
Size: 132 629 196
Fragments arranged by size:
629 196 132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 99 841 957
Size: 99 742 116
Fragments arranged by size:
742 116 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 503 535 957
Size: 503 32 422
Fragments arranged by size:
503 422 32
MscI TGG'CCA
Cuts at: 0 376 957
Size: 376 581
MslI CAynn'nnrTG
Cuts at: 0 142 175 430 586 601 742 957
Size: 142 33 255 156 15 141 215
Fragments arranged by size:
255 215 156 142 141 33 15
MspA1I CmG'CkG
Cuts at: 0 498 694 957
Size: 498 196 263
Fragments arranged by size:
498 263 196
NcoI C'CATG_G
Cuts at: 0 320 431 803 957
Size: 320 111 372 154
Fragments arranged by size:
372 320 154 111
NspI r_CATG'y
Cuts at: 0 429 957
Size: 429 528
PflMI CCAn_nnn'nTGG
Cuts at: 0 446 957
Size: 446 511
PfoI T'CCnGG_A
Cuts at: 0 206 245 957
Size: 206 39 712
Fragments arranged by size:
712 206 39
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 617 649 957
Size: 617 32 308
Fragments arranged by size:
617 308 32
PpuMI rG'GwC_Cy
Cuts at: 0 132 761 957
Size: 132 629 196
Fragments arranged by size:
629 196 132
PvuII CAG'CTG
Cuts at: 0 498 694 957
Size: 498 196 263
Fragments arranged by size:
498 263 196
SfcI C'TryA_G
Cuts at: 0 888 957
Size: 888 69
StyI C'CwwG_G
Cuts at: 0 189 320 431 485 621 803 957
Size: 189 131 111 54 136 182 154
Fragments arranged by size:
189 182 154 136 131 111 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 77 957
Size: 77 880
TatI w'GTAC_w
Cuts at: 0 179 957
Size: 179 778
Tth111I GACn'n_nGTC
Cuts at: 0 517 957
Size: 517 440
Enzymes that do cut:
AarI AleI AloI AlwNI AvrII BamHI BbsI BbvCI
BclI Bpu10I BsaI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BssSI BstEII BstYI Bsu36I
BtgI EaeI EcoO109I FalI Hin4I MscI MslI MspA1I
NcoI NspI PflMI PfoI PpiI PpuMI PvuII SfcI
StyI TaqII TatI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BaeI BanI BanII BbeI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI XbaI XcmI XhoI XmaI XmnI ZraI