Restriction map for
pFN21AE5429
(Linear) (Six-base) MAPSORT of: KIEE5429.seq Check: 4230 from: 1 to: 1005
KIEE5429.orf
With 182 enzymes: SgfI *
January 29, 2014 12:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 32 1005
Size: 32 973
AccI GT'mk_AC
Cuts at: 0 498 1005
Size: 498 507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 383 490 1005
Size: 161 222 107 515
Fragments arranged by size:
515 222 161 107
AlwNI CAG_nnn'CTG
Cuts at: 0 981 1005
Size: 981 24
ApoI r'AATT_y
Cuts at: 0 50 114 788 877 1005
Size: 50 64 674 89 128
Fragments arranged by size:
674 128 89 64 50
BamHI G'GATC_C
Cuts at: 0 868 1005
Size: 868 137
BanI G'GyrC_C
Cuts at: 0 6 1005
Size: 6 999
BanII G_rGCy'C
Cuts at: 0 675 1005
Size: 675 330
BbeI G_GCGC'C
Cuts at: 0 10 1005
Size: 10 995
BclI T'GATC_A
Cuts at: 0 956 1005
Size: 956 49
BfrBI ATG'CAT
Cuts at: 0 898 1005
Size: 898 107
BmgBI CAC'GTC
Cuts at: 0 318 962 1005
Size: 318 644 43
Fragments arranged by size:
644 318 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 5 252 1005
Size: 5 247 753
Fragments arranged by size:
753 247 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1005
Size: 910 95
Bpu10I CC'TnA_GC
Cuts at: 0 194 1005
Size: 194 811
BsaI GGTCTCn'nnnn_
Cuts at: 0 915 1005
Size: 915 90
BsaHI Gr'CG_yC
Cuts at: 0 7 17 1005
Size: 7 10 988
Fragments arranged by size:
988 10 7
BsaWI w'CCGG_w
Cuts at: 0 186 1005
Size: 186 819
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 841 1005
Size: 313 528 164
Fragments arranged by size:
528 313 164
BseYI C'CCAG_C
Cuts at: 0 331 1005
Size: 331 674
BsiHKAI G_wGCw'C
Cuts at: 0 476 947 1005
Size: 476 471 58
Fragments arranged by size:
476 471 58
BsmI GAATG_Cn'
Cuts at: 0 838 1005
Size: 838 167
Bsp1286I G_dGCh'C
Cuts at: 0 476 675 947 1005
Size: 476 199 272 58
Fragments arranged by size:
476 272 199 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 1005
Size: 32 973
BsrDI GCAATG_nn'
Cuts at: 0 597 1005
Size: 597 408
BsrFI r'CCGG_y
Cuts at: 0 162 1005
Size: 162 843
BssHII G'CGCG_C
Cuts at: 0 971 1005
Size: 971 34
BstXI CCAn_nnnn'nTGG
Cuts at: 0 791 1005
Size: 791 214
BstYI r'GATC_y
Cuts at: 0 763 868 977 1005
Size: 763 105 109 28
Fragments arranged by size:
763 109 105 28
BtgI C'CryG_G
Cuts at: 0 558 821 1005
Size: 558 263 184
Fragments arranged by size:
558 263 184
DraI TTT'AAA
Cuts at: 0 344 882 1005
Size: 344 538 123
Fragments arranged by size:
538 344 123
EarI CTCTTCn'nnn_
Cuts at: 0 63 331 359 509 1005
Size: 63 268 28 150 496
Fragments arranged by size:
496 268 150 63 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 383 490 910 1005
Size: 161 222 107 420 95
Fragments arranged by size:
420 222 161 107 95
HaeII r_GCGC'y
Cuts at: 0 10 1005
Size: 10 995
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 360 392 506 538 1005
Size: 360 32 114 32 467
Fragments arranged by size:
467 360 114 32 32
HincII GTy'rAC
Cuts at: 0 103 351 499 1005
Size: 103 248 148 506
Fragments arranged by size:
506 248 148 103
KasI G'GCGC_C
Cuts at: 0 6 1005
Size: 6 999
MfeI C'AATT_G
Cuts at: 0 650 1005
Size: 650 355
MslI CAynn'nnrTG
Cuts at: 0 719 1005
Size: 719 286
MspA1I CmG'CkG
Cuts at: 0 306 1005
Size: 306 699
NaeI GCC'GGC
Cuts at: 0 164 1005
Size: 164 841
NarI GG'CG_CC
Cuts at: 0 7 1005
Size: 7 998
NcoI C'CATG_G
Cuts at: 0 558 821 1005
Size: 558 263 184
Fragments arranged by size:
558 263 184
NgoMIV G'CCGG_C
Cuts at: 0 162 1005
Size: 162 843
NsiI A_TGCA'T
Cuts at: 0 900 1005
Size: 900 105
PfoI T'CCnGG_A
Cuts at: 0 887 1005
Size: 887 118
PshAI GACnn'nnGTC
Cuts at: 0 907 1005
Size: 907 98
PstI C_TGCA'G
Cuts at: 0 171 1005
Size: 171 834
PvuII CAG'CTG
Cuts at: 0 306 1005
Size: 306 699
SalI G'TCGA_C
Cuts at: 0 497 1005
Size: 497 508
SfcI C'TryA_G
Cuts at: 0 167 845 1005
Size: 167 678 160
Fragments arranged by size:
678 167 160
SfoI GGC'GCC
Cuts at: 0 8 1005
Size: 8 997
StyI C'CwwG_G
Cuts at: 0 558 821 1005
Size: 558 263 184
Fragments arranged by size:
558 263 184
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI BamHI BanI BanII
BbeI BclI BfrBI BmgBI BmrI BpmI Bpu10I BsaI
BsaHI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BsrFI BssHII BstXI BstYI BtgI DraI EarI
Eco57MI HaeII Hin4I HincII KasI MfeI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI PfoI PshAI PstI
PvuII SalI SfcI SfoI StyI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbsI BbvCI BcgI BciVI BglI
BglII BlpI Bme1580I BmtI BplI BpuEI BsaAI BsaBI
BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MluI MmeI MscI NdeI
NheI NotI NruI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI