Restriction map for
pFN21AE5428
(Linear) (Six-base) MAPSORT of: KIEE5428.seq Check: 629 from: 1 to: 936
KIEE5428.orf
With 182 enzymes: SgfI *
January 29, 2014 12:00 ..
BanI G'GyrC_C
Cuts at: 0 509 540 936
Size: 509 31 396
Fragments arranged by size:
509 396 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 300 936
Size: 300 636
BbvCI CC'TCA_GC
Cuts at: 0 187 936
Size: 187 749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 526 560 936
Size: 526 34 376
Fragments arranged by size:
526 376 34
BclI T'GATC_A
Cuts at: 0 245 551 936
Size: 245 306 385
Fragments arranged by size:
385 306 245
BlpI GC'TnA_GC
Cuts at: 0 759 936
Size: 759 177
Bme1580I G_kGCm'C
Cuts at: 0 512 936
Size: 512 424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 172 936
Size: 88 84 764
Fragments arranged by size:
764 88 84
Bpu10I CC'TnA_GC
Cuts at: 0 187 665 936
Size: 187 478 271
Fragments arranged by size:
478 271 187
BsaAI yAC'GTr
Cuts at: 0 369 719 936
Size: 369 350 217
Fragments arranged by size:
369 350 217
BsaWI w'CCGG_w
Cuts at: 0 512 936
Size: 512 424
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 936
Size: 184 30 722
Fragments arranged by size:
722 184 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 189 918 936
Size: 189 729 18
Fragments arranged by size:
729 189 18
BseYI C'CCAG_C
Cuts at: 0 62 296 318 413 447 912 936
Size: 62 234 22 95 34 465 24
Fragments arranged by size:
465 234 95 62 34 24 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 591 936
Size: 591 345
BsiHKAI G_wGCw'C
Cuts at: 0 596 936
Size: 596 340
Bsp1286I G_dGCh'C
Cuts at: 0 512 596 936
Size: 512 84 340
Fragments arranged by size:
512 340 84
BspHI T'CATG_A
Cuts at: 0 404 936
Size: 404 532
BsrBI CCG'CTC
Cuts at: 0 686 936
Size: 686 250
BsrDI GCAATG_nn'
Cuts at: 0 659 936
Size: 659 277
BsrFI r'CCGG_y
Cuts at: 0 380 936
Size: 380 556
BtgI C'CryG_G
Cuts at: 0 848 936
Size: 848 88
BtsI GCAGTG_nn'
Cuts at: 0 380 617 677 936
Size: 380 237 60 259
Fragments arranged by size:
380 259 237 60
DraIII CAC_nnn'GTG
Cuts at: 0 332 936
Size: 332 604
EaeI y'GGCC_r
Cuts at: 0 785 936
Size: 785 151
EarI CTCTTCn'nnn_
Cuts at: 0 100 754 918 936
Size: 100 654 164 18
Fragments arranged by size:
654 164 100 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 172 936
Size: 88 84 764
Fragments arranged by size:
764 88 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 535 936
Size: 535 401
HincII GTy'rAC
Cuts at: 0 479 936
Size: 479 457
MslI CAynn'nnrTG
Cuts at: 0 640 847 886 936
Size: 640 207 39 50
Fragments arranged by size:
640 207 50 39
MspA1I CmG'CkG
Cuts at: 0 66 936
Size: 66 870
NaeI GCC'GGC
Cuts at: 0 382 936
Size: 382 554
NcoI C'CATG_G
Cuts at: 0 848 936
Size: 848 88
NgoMIV G'CCGG_C
Cuts at: 0 380 936
Size: 380 556
PflMI CCAn_nnn'nTGG
Cuts at: 0 505 735 936
Size: 505 230 201
Fragments arranged by size:
505 230 201
PshAI GACnn'nnGTC
Cuts at: 0 575 936
Size: 575 361
PstI C_TGCA'G
Cuts at: 0 494 624 693 936
Size: 494 130 69 243
Fragments arranged by size:
494 243 130 69
PvuII CAG'CTG
Cuts at: 0 66 936
Size: 66 870
SfcI C'TryA_G
Cuts at: 0 490 620 689 936
Size: 490 130 69 247
Fragments arranged by size:
490 247 130 69
SpeI A'CTAG_T
Cuts at: 0 223 936
Size: 223 713
StyI C'CwwG_G
Cuts at: 0 203 848 936
Size: 203 645 88
Fragments arranged by size:
645 203 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 439 861 936
Size: 186 253 422 75
Fragments arranged by size:
422 253 186 75
TatI w'GTAC_w
Cuts at: 0 176 936
Size: 176 760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 555 936
Size: 555 381
Enzymes that do cut:
BanI BbsI BbvCI BcgI BclI BlpI Bme1580I BpmI
Bpu10I BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BsrBI BsrDI BsrFI BtgI BtsI DraIII
EaeI EarI Eco57MI EcoNI HincII MslI MspA1I NaeI
NcoI NgoMIV PflMI PshAI PstI PvuII SfcI SpeI
StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BciVI BfrBI BglI BglII BmgBI
BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NarI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI