Restriction map for
pFN21AE5426
(Linear) (Six-base) MAPSORT of: KIEE5426.seq Check: 5880 from: 1 to: 945
KIEE5426.orf
With 182 enzymes: SgfI *
January 29, 2014 12:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 716 855 913 945
Size: 297 419 139 58 32
Fragments arranged by size:
419 297 139 58 32
AfeI AGC'GCT
Cuts at: 0 268 945
Size: 268 677
AhdI GACnn_n'nnGTC
Cuts at: 0 255 945
Size: 255 690
ApaLI G'TGCA_C
Cuts at: 0 751 945
Size: 751 194
ApoI r'AATT_y
Cuts at: 0 31 945
Size: 31 914
BamHI G'GATC_C
Cuts at: 0 508 945
Size: 508 437
BanI G'GyrC_C
Cuts at: 0 940 945
Size: 940 5
BanII G_rGCy'C
Cuts at: 0 412 945
Size: 412 533
BbvCI CC'TCA_GC
Cuts at: 0 856 919 945
Size: 856 63 26
Fragments arranged by size:
856 63 26
BglI GCCn_nnn'nGGC
Cuts at: 0 283 416 434 945
Size: 283 133 18 511
Fragments arranged by size:
511 283 133 18
Bme1580I G_kGCm'C
Cuts at: 0 755 945
Size: 755 190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 945
Size: 168 777
Bpu10I CC'TnA_GC
Cuts at: 0 856 919 945
Size: 856 63 26
Fragments arranged by size:
856 63 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 945
Size: 766 179
BsaAI yAC'GTr
Cuts at: 0 369 945
Size: 369 576
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 945
Size: 4 30 911
Fragments arranged by size:
911 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 628 637 945
Size: 628 9 308
Fragments arranged by size:
628 308 9
BseYI C'CCAG_C
Cuts at: 0 3 117 263 624 945
Size: 3 114 146 361 321
Fragments arranged by size:
361 321 146 114 3
BsiEI CG_ry'CG
Cuts at: 0 363 945
Size: 363 582
BsiHKAI G_wGCw'C
Cuts at: 0 404 755 945
Size: 404 351 190
Fragments arranged by size:
404 351 190
Bsp1286I G_dGCh'C
Cuts at: 0 404 412 755 945
Size: 404 8 343 190
Fragments arranged by size:
404 343 190 8
BspHI T'CATG_A
Cuts at: 0 404 945
Size: 404 541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 260 945
Size: 134 126 685
Fragments arranged by size:
685 134 126
BstEII G'GTnAC_C
Cuts at: 0 474 945
Size: 474 471
BstYI r'GATC_y
Cuts at: 0 209 508 518 683 945
Size: 209 299 10 165 262
Fragments arranged by size:
299 262 209 165 10
Bsu36I CC'TnA_GG
Cuts at: 0 877 936 945
Size: 877 59 9
Fragments arranged by size:
877 59 9
BtgI C'CryG_G
Cuts at: 0 221 412 945
Size: 221 191 533
Fragments arranged by size:
533 221 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 258 945
Size: 258 687
BtsI GCAGTG_nn'
Cuts at: 0 112 619 945
Size: 112 507 326
Fragments arranged by size:
507 326 112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 533 568 945
Size: 533 35 377
Fragments arranged by size:
533 377 35
EaeI y'GGCC_r
Cuts at: 0 137 224 245 371 482 668 824 945
Size: 137 87 21 126 111 186 156 121
Fragments arranged by size:
186 156 137 126 121 111 87 21
EarI CTCTTCn'nnn_
Cuts at: 0 85 187 901 945
Size: 85 102 714 44
Fragments arranged by size:
714 102 85 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 297 716 855 913 945
Size: 168 129 419 139 58 32
Fragments arranged by size:
419 168 139 129 58 32
EcoO109I rG'GnC_Cy
Cuts at: 0 793 911 945
Size: 793 118 34
Fragments arranged by size:
793 118 34
EcoRI G'AATT_C
Cuts at: 0 31 945
Size: 31 914
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 930 945
Size: 930 15
HaeII r_GCGC'y
Cuts at: 0 270 945
Size: 270 675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 945
Size: 241 32 672
Fragments arranged by size:
672 241 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 516 682 945
Size: 93 423 166 263
Fragments arranged by size:
423 263 166 93
MscI TGG'CCA
Cuts at: 0 139 226 373 826 945
Size: 139 87 147 453 119
Fragments arranged by size:
453 147 139 119 87
MslI CAynn'nnrTG
Cuts at: 0 22 172 349 466 820 945
Size: 22 150 177 117 354 125
Fragments arranged by size:
354 177 150 125 117 22
MspA1I CmG'CkG
Cuts at: 0 583 945
Size: 583 362
NspI r_CATG'y
Cuts at: 0 243 945
Size: 243 702
PasI CC'CwG_GG
Cuts at: 0 591 945
Size: 591 354
PflMI CCAn_nnn'nTGG
Cuts at: 0 146 945
Size: 146 799
PpuMI rG'GwC_Cy
Cuts at: 0 793 911 945
Size: 793 118 34
Fragments arranged by size:
793 118 34
PvuII CAG'CTG
Cuts at: 0 583 945
Size: 583 362
SapI GCTCTTCn'nnn_
Cuts at: 0 85 945
Size: 85 860
SmlI C'TyrA_G
Cuts at: 0 781 945
Size: 781 164
SphI G_CATG'C
Cuts at: 0 243 945
Size: 243 702
StuI AGG'CCT
Cuts at: 0 712 945
Size: 712 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 343 447 945
Size: 277 66 104 498
Fragments arranged by size:
498 277 104 66
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 945
Size: 279 666
Enzymes that do cut:
AcuI AfeI AhdI ApaLI ApoI BamHI BanI BanII
BbvCI BglI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BstEII
BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I MmeI MscI
MslI MspA1I NspI PasI PflMI PpuMI PvuII SapI
SmlI SphI StuI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BsaI
BsaBI BsaHI BsaWI BsgI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I ClaI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StyI SwaI TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI