Restriction map for
pFN21AE5421
(Linear) (Six-base) MAPSORT of: KIEE5421.seq Check: 5123 from: 1 to: 936
KIEE5421.orf
With 182 enzymes: SgfI *
January 29, 2014 11:59 ..
AccI GT'mk_AC
Cuts at: 0 764 936
Size: 764 172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 824 936
Size: 347 477 112
Fragments arranged by size:
477 347 112
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 560 593 936
Size: 560 33 343
Fragments arranged by size:
560 343 33
BamHI G'GATC_C
Cuts at: 0 150 936
Size: 150 786
BbsI GAAGACnn'nnnn_
Cuts at: 0 812 816 936
Size: 812 4 120
Fragments arranged by size:
812 120 4
BglI GCCn_nnn'nGGC
Cuts at: 0 266 936
Size: 266 670
BmrI ACTGGGnnnn_n'
Cuts at: 0 457 936
Size: 457 479
BsaAI yAC'GTr
Cuts at: 0 147 936
Size: 147 789
BsaBI GATnn'nnATC
Cuts at: 0 214 936
Size: 214 722
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 915 936
Size: 915 21
BseYI C'CCAG_C
Cuts at: 0 540 936
Size: 540 396
BsiEI CG_ry'CG
Cuts at: 0 360 936
Size: 360 576
BsmBI CGTCTCn'nnnn_
Cuts at: 0 163 936
Size: 163 773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 494 936
Size: 494 442
BsrBI CCG'CTC
Cuts at: 0 794 936
Size: 794 142
BsrGI T'GTAC_A
Cuts at: 0 212 562 936
Size: 212 350 374
Fragments arranged by size:
374 350 212
BstYI r'GATC_y
Cuts at: 0 150 812 936
Size: 150 662 124
Fragments arranged by size:
662 150 124
Bsu36I CC'TnA_GG
Cuts at: 0 871 936
Size: 871 65
BtgI C'CryG_G
Cuts at: 0 314 347 936
Size: 314 33 589
Fragments arranged by size:
589 314 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 548 936
Size: 548 388
DrdI GACnn_nn'nnGTC
Cuts at: 0 450 936
Size: 450 486
EaeI y'GGCC_r
Cuts at: 0 125 258 368 651 936
Size: 125 133 110 283 285
Fragments arranged by size:
285 283 133 125 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 824 936
Size: 347 477 112
Fragments arranged by size:
477 347 112
EcoNI CCTnn'n_nnAGG
Cuts at: 0 501 936
Size: 501 435
HindIII A'AGCT_T
Cuts at: 0 297 936
Size: 297 639
MscI TGG'CCA
Cuts at: 0 370 936
Size: 370 566
MslI CAynn'nnrTG
Cuts at: 0 145 169 936
Size: 145 24 767
Fragments arranged by size:
767 145 24
MspA1I CmG'CkG
Cuts at: 0 802 936
Size: 802 134
NcoI C'CATG_G
Cuts at: 0 314 347 936
Size: 314 33 589
Fragments arranged by size:
589 314 33
NdeI CA'TA_TG
Cuts at: 0 677 936
Size: 677 259
PmlI CAC'GTG
Cuts at: 0 147 936
Size: 147 789
PstI C_TGCA'G
Cuts at: 0 505 936
Size: 505 431
PvuII CAG'CTG
Cuts at: 0 802 936
Size: 802 134
SfcI C'TryA_G
Cuts at: 0 501 734 864 936
Size: 501 233 130 72
Fragments arranged by size:
501 233 130 72
SspI AAT'ATT
Cuts at: 0 71 936
Size: 71 865
StuI AGG'CCT
Cuts at: 0 706 936
Size: 706 230
StyI C'CwwG_G
Cuts at: 0 314 347 464 936
Size: 314 33 117 472
Fragments arranged by size:
472 314 117 33
TatI w'GTAC_w
Cuts at: 0 173 212 562 601 936
Size: 173 39 350 39 335
Fragments arranged by size:
350 335 173 39 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 275 307 936
Size: 275 32 629
Fragments arranged by size:
629 275 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 462 936
Size: 462 474
Enzymes that do cut:
AccI AcuI BaeI BamHI BbsI BglI BmrI BsaAI
BsaBI BseRI BseYI BsiEI BsmBI BspMI BsrBI BsrGI
BstYI Bsu36I BtgI BtgZI DrdI EaeI Eco57MI EcoNI
HindIII MscI MslI MspA1I NcoI NdeI PmlI PstI
PvuII SfcI SspI StuI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BanI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BsgI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtsI ClaI CspCI DraI DraIII EagI
EarI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SwaI TaqII Tth111I XbaI
XhoI XmaI XmnI ZraI