Restriction map for
pFN21AE5418
(Linear) (Six-base) MAPSORT of: KIEE5418.seq Check: 7478 from: 1 to: 954
KIEE5418.orf
With 182 enzymes: SgfI *
January 29, 2014 11:59 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 102 144 867 954
Size: 102 42 723 87
Fragments arranged by size:
723 102 87 42
ApoI r'AATT_y
Cuts at: 0 338 904 954
Size: 338 566 50
Fragments arranged by size:
566 338 50
AvaI C'yCGr_G
Cuts at: 0 120 343 954
Size: 120 223 611
Fragments arranged by size:
611 223 120
BamHI G'GATC_C
Cuts at: 0 722 954
Size: 722 232
BanI G'GyrC_C
Cuts at: 0 275 954
Size: 275 679
BbsI GAAGACnn'nnnn_
Cuts at: 0 432 447 843 954
Size: 432 15 396 111
Fragments arranged by size:
432 396 111 15
BlpI GC'TnA_GC
Cuts at: 0 532 954
Size: 532 422
BmgBI CAC'GTC
Cuts at: 0 477 954
Size: 477 477
BsaBI GATnn'nnATC
Cuts at: 0 628 954
Size: 628 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 502 954
Size: 397 105 452
Fragments arranged by size:
452 397 105
BsiHKAI G_wGCw'C
Cuts at: 0 26 306 954
Size: 26 280 648
Fragments arranged by size:
648 280 26
BsmI GAATG_Cn'
Cuts at: 0 385 564 954
Size: 385 179 390
Fragments arranged by size:
390 385 179
Bsp1286I G_dGCh'C
Cuts at: 0 26 306 954
Size: 26 280 648
Fragments arranged by size:
648 280 26
BspHI T'CATG_A
Cuts at: 0 467 954
Size: 467 487
BssHII G'CGCG_C
Cuts at: 0 45 954
Size: 45 909
BstAPI GCAn_nnn'nTGC
Cuts at: 0 102 382 954
Size: 102 280 572
Fragments arranged by size:
572 280 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 613 954
Size: 613 341
BstYI r'GATC_y
Cuts at: 0 722 954
Size: 722 232
BtgI C'CryG_G
Cuts at: 0 794 954
Size: 794 160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 580 615 954
Size: 580 35 339
Fragments arranged by size:
580 339 35
DraIII CAC_nnn'GTG
Cuts at: 0 480 954
Size: 480 474
EaeI y'GGCC_r
Cuts at: 0 797 954
Size: 797 157
EarI CTCTTCn'nnn_
Cuts at: 0 436 954
Size: 436 518
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 954
Size: 28 926
EcoO109I rG'GnC_Cy
Cuts at: 0 141 864 954
Size: 141 723 90
Fragments arranged by size:
723 141 90
EcoRI G'AATT_C
Cuts at: 0 338 954
Size: 338 616
HaeII r_GCGC'y
Cuts at: 0 34 954
Size: 34 920
HindIII A'AGCT_T
Cuts at: 0 932 954
Size: 932 22
MslI CAynn'nnrTG
Cuts at: 0 247 954
Size: 247 707
MspA1I CmG'CkG
Cuts at: 0 42 954
Size: 42 912
NcoI C'CATG_G
Cuts at: 0 794 954
Size: 794 160
PasI CC'CwG_GG
Cuts at: 0 8 954
Size: 8 946
PflMI CCAn_nnn'nTGG
Cuts at: 0 550 954
Size: 550 404
PfoI T'CCnGG_A
Cuts at: 0 137 954
Size: 137 817
PpuMI rG'GwC_Cy
Cuts at: 0 141 954
Size: 141 813
PshAI GACnn'nnGTC
Cuts at: 0 847 954
Size: 847 107
PstI C_TGCA'G
Cuts at: 0 98 954
Size: 98 856
ScaI AGT'ACT
Cuts at: 0 487 954
Size: 487 467
SfcI C'TryA_G
Cuts at: 0 94 954
Size: 94 860
SmaI CCC'GGG
Cuts at: 0 122 954
Size: 122 832
StyI C'CwwG_G
Cuts at: 0 794 954
Size: 794 160
TatI w'GTAC_w
Cuts at: 0 485 954
Size: 485 469
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 587 619 954
Size: 587 32 335
Fragments arranged by size:
587 335 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 734 954
Size: 734 220
XmaI C'CCGG_G
Cuts at: 0 120 954
Size: 120 834
XmnI GAAnn'nnTTC
Cuts at: 0 262 934 954
Size: 262 672 20
Fragments arranged by size:
672 262 20
Enzymes that do cut:
AlwNI ApoI AvaI BamHI BanI BbsI BlpI BmgBI
BsaBI BsgI BsiHKAI BsmI Bsp1286I BspHI BssHII BstAPI
BstXI BstYI BtgI CspCI DraIII EaeI EarI EciI
EcoO109I EcoRI HaeII HindIII MslI MspA1I NcoI PasI
PflMI PfoI PpuMI PshAI PstI ScaI SfcI SmaI
StyI TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI DraI DrdI EagI
EcoICRI Eco57MI EcoNI EcoRV FalI FseI FspI FspAI
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XhoI ZraI