Restriction map for
pFN21AE5417
(Linear) (Six-base) MAPSORT of: KIEE5417.seq Check: 5589 from: 1 to: 987
KIEE5417.orf
With 182 enzymes: SgfI *
January 29, 2014 11:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 126 987
Size: 126 861
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 987
Size: 886 101
AleI CACnn'nnGTG
Cuts at: 0 637 987
Size: 637 350
AlwNI CAG_nnn'CTG
Cuts at: 0 187 987
Size: 187 800
AvaI C'yCGr_G
Cuts at: 0 650 987
Size: 650 337
BanI G'GyrC_C
Cuts at: 0 679 987
Size: 679 308
BbsI GAAGACnn'nnnn_
Cuts at: 0 246 987
Size: 246 741
BglI GCCn_nnn'nGGC
Cuts at: 0 593 987
Size: 593 394
BlpI GC'TnA_GC
Cuts at: 0 758 987
Size: 758 229
Bme1580I G_kGCm'C
Cuts at: 0 471 682 987
Size: 471 211 305
Fragments arranged by size:
471 305 211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 987
Size: 385 602
BsaHI Gr'CG_yC
Cuts at: 0 17 987
Size: 17 970
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 386 416 987
Size: 386 30 571
Fragments arranged by size:
571 386 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 253 331 793 826 987
Size: 196 57 78 462 33 161
Fragments arranged by size:
462 196 161 78 57 33
BseYI C'CCAG_C
Cuts at: 0 37 66 226 609 987
Size: 37 29 160 383 378
Fragments arranged by size:
383 378 160 37 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 987
Size: 160 827
BsiEI CG_ry'CG
Cuts at: 0 537 987
Size: 537 450
Bsp1286I G_dGCh'C
Cuts at: 0 471 682 987
Size: 471 211 305
Fragments arranged by size:
471 305 211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 126 987
Size: 126 861
BsrBI CCG'CTC
Cuts at: 0 158 987
Size: 158 829
BsrFI r'CCGG_y
Cuts at: 0 131 547 987
Size: 131 416 440
Fragments arranged by size:
440 416 131
BstAPI GCAn_nnn'nTGC
Cuts at: 0 771 987
Size: 771 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 198 987
Size: 198 789
BstYI r'GATC_y
Cuts at: 0 183 201 242 646 861 987
Size: 183 18 41 404 215 126
Fragments arranged by size:
404 215 183 126 41 18
BtgI C'CryG_G
Cuts at: 0 28 197 987
Size: 28 169 790
Fragments arranged by size:
790 169 28
EaeI y'GGCC_r
Cuts at: 0 444 833 987
Size: 444 389 154
Fragments arranged by size:
444 389 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 886 987
Size: 385 501 101
Fragments arranged by size:
501 385 101
EcoNI CCTnn'n_nnAGG
Cuts at: 0 367 808 987
Size: 367 441 179
Fragments arranged by size:
441 367 179
EcoO109I rG'GnC_Cy
Cuts at: 0 34 595 606 825 987
Size: 34 561 11 219 162
Fragments arranged by size:
561 219 162 34 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 677 709 987
Size: 677 32 278
Fragments arranged by size:
677 278 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 987
Size: 334 653
MslI CAynn'nnrTG
Cuts at: 0 196 637 987
Size: 196 441 350
Fragments arranged by size:
441 350 196
MspA1I CmG'CkG
Cuts at: 0 30 41 85 93 144 336 730 987
Size: 30 11 44 8 51 192 394 257
Fragments arranged by size:
394 257 192 51 44 30 11 8
NaeI GCC'GGC
Cuts at: 0 549 987
Size: 549 438
NcoI C'CATG_G
Cuts at: 0 197 987
Size: 197 790
NgoMIV G'CCGG_C
Cuts at: 0 547 987
Size: 547 440
NspI r_CATG'y
Cuts at: 0 774 987
Size: 774 213
PasI CC'CwG_GG
Cuts at: 0 666 987
Size: 666 321
PfoI T'CCnGG_A
Cuts at: 0 341 429 513 821 901 987
Size: 341 88 84 308 80 86
Fragments arranged by size:
341 308 88 86 84 80
PpuMI rG'GwC_Cy
Cuts at: 0 34 606 825 987
Size: 34 572 219 162
Fragments arranged by size:
572 219 162 34
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 256 288 987
Size: 256 32 699
Fragments arranged by size:
699 256 32
PstI C_TGCA'G
Cuts at: 0 224 987
Size: 224 763
PvuII CAG'CTG
Cuts at: 0 93 336 730 987
Size: 93 243 394 257
Fragments arranged by size:
394 257 243 93
SacII CC_GC'GG
Cuts at: 0 31 987
Size: 31 956
SanDI GG'GwC_CC
Cuts at: 0 34 606 987
Size: 34 572 381
Fragments arranged by size:
572 381 34
SfcI C'TryA_G
Cuts at: 0 220 987
Size: 220 767
SmlI C'TyrA_G
Cuts at: 0 650 987
Size: 650 337
StyI C'CwwG_G
Cuts at: 0 109 197 987
Size: 109 88 790
Fragments arranged by size:
790 109 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 722 987
Size: 722 265
TatI w'GTAC_w
Cuts at: 0 263 987
Size: 263 724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 639 663 987
Size: 639 24 324
Fragments arranged by size:
639 324 24
XhoI C'TCGA_G
Cuts at: 0 650 987
Size: 650 337
XmnI GAAnn'nnTTC
Cuts at: 0 274 581 987
Size: 274 307 406
Fragments arranged by size:
406 307 274
Enzymes that do cut:
AarI AcuI AleI AlwNI AvaI BanI BbsI BglI
BlpI Bme1580I BpmI BsaHI BsaXI BseRI BseYI BsgI
BsiEI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI BstYI
BtgI EaeI Eco57MI EcoNI EcoO109I Hin4I MmeI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PfoI PpuMI
PsrI PstI PvuII SacII SanDI SfcI SmlI StyI
TaqII TatI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NarI NdeI
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XmaI ZraI