(Linear) (Six-base) MAPSORT of: KIEE5414.seq  Check: 8172  from: 1  to: 1047



KIEE5414.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:59  ..

AccI GT'mk_AC

Cuts at:      0     347    1047 
   Size:        347     700
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     852     940    1047 
   Size:         58     794      88     107

  Fragments arranged by size: 

                 794     107      88      58
 
AflIII A'CryG_T

Cuts at:      0     510    1047 
   Size:        510     537
 
AlwNI CAG_nnn'CTG

Cuts at:      0      91    1047 
   Size:         91     956
 
ApoI r'AATT_y

Cuts at:      0     391     911    1036    1047 
   Size:        391     520     125      11

  Fragments arranged by size: 

                 520     391     125      11
 
AseI AT'TA_AT

Cuts at:      0     593    1047 
   Size:        593     454
 
AvaI C'yCGr_G

Cuts at:      0    1010    1047 
   Size:       1010      37
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     673    1047 
   Size:        673     374
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1014    1047 
   Size:       1014      33
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     956    1047 
   Size:        956      91
 
BsaBI GATnn'nnATC

Cuts at:      0     433    1047 
   Size:        433     614
 
BseYI C'CCAG_C

Cuts at:      0      91     762    1047 
   Size:         91     671     285

  Fragments arranged by size: 

                 671     285      91
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     819    1047 
   Size:        819     228
 
BsrBI CCG'CTC

Cuts at:      0      23    1047 
   Size:         23    1024
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     546    1047 
   Size:        546     501
 
BtsI GCAGTG_nn'

Cuts at:      0     678    1047 
   Size:        678     369
 
DraI TTT'AAA

Cuts at:      0     663    1047 
   Size:        663     384
 
DraIII CAC_nnn'GTG

Cuts at:      0     206    1047 
   Size:        206     841
 
EarI CTCTTCn'nnn_

Cuts at:      0     356     519     696     959    1047 
   Size:        356     163     177     263      88

  Fragments arranged by size: 

                 356     263     177     163      88
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     134    1047 
   Size:        134     913
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     852     940    1047 
   Size:         58     794      88     107

  Fragments arranged by size: 

                 794     107      88      58
 
EcoO109I rG'GnC_Cy

Cuts at:      0     711    1047 
   Size:        711     336
 
EcoRI G'AATT_C

Cuts at:      0     911    1047 
   Size:        911     136
 
HaeII r_GCGC'y

Cuts at:      0      92    1047 
   Size:         92     955
 
MfeI C'AATT_G

Cuts at:      0     773    1047 
   Size:        773     274
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     144    1047 
   Size:        144     903
 
MspA1I CmG'CkG

Cuts at:      0      32      61     183     222    1047 
   Size:         32      29     122      39     825

  Fragments arranged by size: 

                 825     122      39      32      29
 
NspI r_CATG'y

Cuts at:      0     514    1047 
   Size:        514     533
 
PciI A'CATG_T

Cuts at:      0     510    1047 
   Size:        510     537
 
PpuMI rG'GwC_Cy

Cuts at:      0     711    1047 
   Size:        711     336
 
PstI C_TGCA'G

Cuts at:      0      87    1047 
   Size:         87     960
 
SfcI C'TryA_G

Cuts at:      0      83     383    1047 
   Size:         83     300     664

  Fragments arranged by size: 

                 664     300      83
 
SmaI CCC'GGG

Cuts at:      0    1012    1047 
   Size:       1012      35
 
XmaI C'CCGG_G

Cuts at:      0    1010    1047 
   Size:       1010      37
 
XmnI GAAnn'nnTTC

Cuts at:      0      71     737    1047 
   Size:         71     666     310

  Fragments arranged by size: 

                 666     310      71
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApoI     AseI     AvaI     BbsI
    BciVI     BsaI    BsaBI    BseYI    BspMI    BsrBI    BtgZI     BtsI
     DraI   DraIII     EarI     EciI  Eco57MI EcoO109I    EcoRI    HaeII
     MfeI     MmeI   MspA1I     NspI     PciI    PpuMI     PstI     SfcI
     SmaI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI     DrdI     EaeI     EagI  EcoICRI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     ZraI