Restriction map for
pFN21AE5412
(Linear) (Six-base) MAPSORT of: KIEE5412.seq Check: 6822 from: 1 to: 930
KIEE5412.orf
With 182 enzymes: SgfI *
January 29, 2014 11:59 ..
AclI AA'CG_TT
Cuts at: 0 221 930
Size: 221 709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 930
Size: 142 788
AflIII A'CryG_T
Cuts at: 0 203 930
Size: 203 727
AhdI GACnn_n'nnGTC
Cuts at: 0 50 930
Size: 50 880
AleI CACnn'nnGTG
Cuts at: 0 16 930
Size: 16 914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 930
Size: 397 533
ApaLI G'TGCA_C
Cuts at: 0 568 930
Size: 568 362
ApoI r'AATT_y
Cuts at: 0 25 930
Size: 25 905
BbvCI CC'TCA_GC
Cuts at: 0 152 930
Size: 152 778
BclI T'GATC_A
Cuts at: 0 101 128 930
Size: 101 27 802
Fragments arranged by size:
802 101 27
Bme1580I G_kGCm'C
Cuts at: 0 572 930
Size: 572 358
BmgBI CAC'GTC
Cuts at: 0 204 930
Size: 204 726
Bpu10I CC'TnA_GC
Cuts at: 0 152 930
Size: 152 778
BsaI GGTCTCn'nnnn_
Cuts at: 0 771 930
Size: 771 159
BsaAI yAC'GTr
Cuts at: 0 321 930
Size: 321 609
BsaBI GATnn'nnATC
Cuts at: 0 142 930
Size: 142 788
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 204 930
Size: 204 726
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 551 930
Size: 310 241 379
Fragments arranged by size:
379 310 241
BsiHKAI G_wGCw'C
Cuts at: 0 572 930
Size: 572 358
Bsp1286I G_dGCh'C
Cuts at: 0 572 930
Size: 572 358
BsrDI GCAATG_nn'
Cuts at: 0 305 930
Size: 305 625
BsrFI r'CCGG_y
Cuts at: 0 367 930
Size: 367 563
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 930
Size: 237 693
BstYI r'GATC_y
Cuts at: 0 534 930
Size: 534 396
BtsI GCAGTG_nn'
Cuts at: 0 298 930
Size: 298 632
EaeI y'GGCC_r
Cuts at: 0 365 930
Size: 365 565
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 930
Size: 142 788
EcoNI CCTnn'n_nnAGG
Cuts at: 0 354 930
Size: 354 576
EcoO109I rG'GnC_Cy
Cuts at: 0 719 752 930
Size: 719 33 178
Fragments arranged by size:
719 178 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 36 68 440 472 557 589 849 881
Size: 36 32 372 32 85 32 260 32
Cuts at: 881 930
Size: 49
Fragments arranged by size:
372 260 85 49 36 32 32 32
32
HincII GTy'rAC
Cuts at: 0 897 930
Size: 897 33
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 930
Size: 242 688
MslI CAynn'nnrTG
Cuts at: 0 16 430 763 930
Size: 16 414 333 167
Fragments arranged by size:
414 333 167 16
NaeI GCC'GGC
Cuts at: 0 369 930
Size: 369 561
NdeI CA'TA_TG
Cuts at: 0 918 930
Size: 918 12
NgoMIV G'CCGG_C
Cuts at: 0 367 930
Size: 367 563
NspI r_CATG'y
Cuts at: 0 678 930
Size: 678 252
PmlI CAC'GTG
Cuts at: 0 321 930
Size: 321 609
PpuMI rG'GwC_Cy
Cuts at: 0 719 752 930
Size: 719 33 178
Fragments arranged by size:
719 178 33
SanDI GG'GwC_CC
Cuts at: 0 719 752 930
Size: 719 33 178
Fragments arranged by size:
719 178 33
SfcI C'TryA_G
Cuts at: 0 61 861 930
Size: 61 800 69
Fragments arranged by size:
800 69 61
SphI G_CATG'C
Cuts at: 0 678 930
Size: 678 252
SspI AAT'ATT
Cuts at: 0 120 930
Size: 120 810
TatI w'GTAC_w
Cuts at: 0 170 930
Size: 170 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 272 304 399 431 822 854 930
Size: 272 32 95 32 391 32 76
Fragments arranged by size:
391 272 95 76 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 273 556 930
Size: 273 283 374
Fragments arranged by size:
374 283 273
XmnI GAAnn'nnTTC
Cuts at: 0 703 930
Size: 703 227
Enzymes that do cut:
AclI AcuI AflIII AhdI AleI AlfI ApaLI ApoI
BbvCI BclI Bme1580I BmgBI Bpu10I BsaI BsaAI BsaBI
BseRI BsgI BsiHKAI Bsp1286I BsrDI BsrFI BstXI BstYI
BtsI EaeI Eco57MI EcoNI EcoO109I Hin4I HincII MmeI
MslI NaeI NdeI NgoMIV NspI PmlI PpuMI SanDI
SfcI SphI SspI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AloI
AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BfrBI
BglI BglII BlpI BmrI BmtI BplI BpmI BpuEI
BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MscI MspA1I NarI NcoI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI StuI StyI SwaI TaqII
Tth111I XbaI XhoI XmaI ZraI