Restriction map for
pFN21AE5410
(Linear) (Six-base) MAPSORT of: KIEE5410.seq Check: 7026 from: 1 to: 936
KIEE5410.orf
With 182 enzymes: SgfI *
January 29, 2014 11:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 497 501 936
Size: 497 4 435
Fragments arranged by size:
497 435 4
AccI GT'mk_AC
Cuts at: 0 767 936
Size: 767 169
ApaI G_GGCC'C
Cuts at: 0 256 936
Size: 256 680
AvaI C'yCGr_G
Cuts at: 0 54 936
Size: 54 882
BanII G_rGCy'C
Cuts at: 0 256 926 936
Size: 256 670 10
Fragments arranged by size:
670 256 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 78 268 569 597 936
Size: 78 190 301 28 339
Fragments arranged by size:
339 301 190 78 28
BbvCI CC'TCA_GC
Cuts at: 0 670 936
Size: 670 266
BglII A'GATC_T
Cuts at: 0 293 936
Size: 293 643
Bme1580I G_kGCm'C
Cuts at: 0 256 838 936
Size: 256 582 98
Fragments arranged by size:
582 256 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 936
Size: 757 179
Bpu10I CC'TnA_GC
Cuts at: 0 670 936
Size: 670 266
BsaI GGTCTCn'nnnn_
Cuts at: 0 823 936
Size: 823 113
BsaAI yAC'GTr
Cuts at: 0 363 936
Size: 363 573
BsaBI GATnn'nnATC
Cuts at: 0 298 936
Size: 298 638
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 936
Size: 28 908
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 708 936
Size: 708 228
BseYI C'CCAG_C
Cuts at: 0 537 906 936
Size: 537 369 30
Fragments arranged by size:
537 369 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 936
Size: 469 467
BsiEI CG_ry'CG
Cuts at: 0 507 936
Size: 507 429
BsmI GAATG_Cn'
Cuts at: 0 892 936
Size: 892 44
Bsp1286I G_dGCh'C
Cuts at: 0 256 838 926 936
Size: 256 582 88 10
Fragments arranged by size:
582 256 88 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 497 501 721 936
Size: 497 4 220 215
Fragments arranged by size:
497 220 215 4
BsrDI GCAATG_nn'
Cuts at: 0 758 936
Size: 758 178
BssSI C'ACGA_G
Cuts at: 0 216 936
Size: 216 720
BstEII G'GTnAC_C
Cuts at: 0 735 882 936
Size: 735 147 54
Fragments arranged by size:
735 147 54
BstYI r'GATC_y
Cuts at: 0 293 581 936
Size: 293 288 355
Fragments arranged by size:
355 293 288
Bsu36I CC'TnA_GG
Cuts at: 0 868 936
Size: 868 68
BtsI GCAGTG_nn'
Cuts at: 0 671 836 936
Size: 671 165 100
Fragments arranged by size:
671 165 100
DrdI GACnn_nn'nnGTC
Cuts at: 0 286 936
Size: 286 650
EarI CTCTTCn'nnn_
Cuts at: 0 79 748 826 936
Size: 79 669 78 110
Fragments arranged by size:
669 110 79 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 708 936
Size: 708 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 936
Size: 757 179
EcoO109I rG'GnC_Cy
Cuts at: 0 252 534 710 936
Size: 252 282 176 226
Fragments arranged by size:
282 252 226 176
HincII GTy'rAC
Cuts at: 0 6 243 936
Size: 6 237 693
Fragments arranged by size:
693 237 6
HindIII A'AGCT_T
Cuts at: 0 701 936
Size: 701 235
HpaI GTT'AAC
Cuts at: 0 6 936
Size: 6 930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 936
Size: 209 727
MspA1I CmG'CkG
Cuts at: 0 423 936
Size: 423 513
PasI CC'CwG_GG
Cuts at: 0 312 936
Size: 312 624
PflMI CCAn_nnn'nTGG
Cuts at: 0 237 659 936
Size: 237 422 277
Fragments arranged by size:
422 277 237
PfoI T'CCnGG_A
Cuts at: 0 275 936
Size: 275 661
PpuMI rG'GwC_Cy
Cuts at: 0 534 710 936
Size: 534 176 226
Fragments arranged by size:
534 226 176
PspOMI G'GGCC_C
Cuts at: 0 252 936
Size: 252 684
PvuI CG_AT'CG
Cuts at: 0 507 936
Size: 507 429
PvuII CAG'CTG
Cuts at: 0 423 936
Size: 423 513
SfcI C'TryA_G
Cuts at: 0 747 936
Size: 747 189
SmaI CCC'GGG
Cuts at: 0 56 936
Size: 56 880
TatI w'GTAC_w
Cuts at: 0 170 936
Size: 170 766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 11 43 113 145 936
Size: 11 32 70 32 791
Fragments arranged by size:
791 70 32 32 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 273 936
Size: 273 663
XmaI C'CCGG_G
Cuts at: 0 54 936
Size: 54 882
Enzymes that do cut:
AarI AccI ApaI AvaI BanII BbsI BbvCI BglII
Bme1580I BpmI Bpu10I BsaI BsaAI BsaBI BsaXI BseRI
BseYI BsgI BsiEI BsmI Bsp1286I BspMI BsrDI BssSI
BstEII BstYI Bsu36I BtsI DrdI EarI EciI Eco57MI
EcoO109I HincII HindIII HpaI MmeI MspA1I PasI PflMI
PfoI PpuMI PspOMI PvuI PvuII SfcI SmaI TatI
TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI
BciVI BclI BfrBI BglI BlpI BmgBI BmrI BmtI
BplI BpuEI BsaHI BsaWI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstXI
BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII EaeI
EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I KasI KpnI MfeI MluI MscI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII Tth111I XbaI XhoI XmnI
ZraI