Restriction map for
pFN21AE5405
(Linear) (Six-base) MAPSORT of: KIEE5405.seq Check: 3451 from: 1 to: 996
KIEE5405.orf
With 182 enzymes: SgfI *
January 29, 2014 11:59 ..
Acc65I G'GTAC_C
Cuts at: 0 707 996
Size: 707 289
AflIII A'CryG_T
Cuts at: 0 199 996
Size: 199 797
AhdI GACnn_n'nnGTC
Cuts at: 0 855 996
Size: 855 141
AleI CACnn'nnGTG
Cuts at: 0 112 996
Size: 112 884
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 718 750 804 836 996
Size: 718 32 54 32 160
Fragments arranged by size:
718 160 54 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 954 996
Size: 954 42
ApaI G_GGCC'C
Cuts at: 0 18 996
Size: 18 978
AvaI C'yCGr_G
Cuts at: 0 17 28 558 996
Size: 17 11 530 438
Fragments arranged by size:
530 438 17 11
BamHI G'GATC_C
Cuts at: 0 419 513 743 996
Size: 419 94 230 253
Fragments arranged by size:
419 253 230 94
BanI G'GyrC_C
Cuts at: 0 54 61 106 502 547 707 996
Size: 54 7 45 396 45 160 289
Fragments arranged by size:
396 289 160 54 45 45 7
BanII G_rGCy'C
Cuts at: 0 18 308 316 370 544 996
Size: 18 290 8 54 174 452
Fragments arranged by size:
452 290 174 54 18 8
BbeI G_GCGC'C
Cuts at: 0 551 996
Size: 551 445
BbsI GAAGACnn'nnnn_
Cuts at: 0 854 996
Size: 854 142
BbvCI CC'TCA_GC
Cuts at: 0 274 655 996
Size: 274 381 341
Fragments arranged by size:
381 341 274
BclI T'GATC_A
Cuts at: 0 773 996
Size: 773 223
BglI GCCn_nnn'nGGC
Cuts at: 0 22 88 996
Size: 22 66 908
Fragments arranged by size:
908 66 22
Bme1580I G_kGCm'C
Cuts at: 0 18 59 109 505 996
Size: 18 41 50 396 491
Fragments arranged by size:
491 396 50 41 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 460 492 996
Size: 460 32 504
Fragments arranged by size:
504 460 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 286 918 996
Size: 286 632 78
Fragments arranged by size:
632 286 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 148 636 820 888 996
Size: 135 13 488 184 68 108
Fragments arranged by size:
488 184 135 108 68 13
Bpu10I CC'TnA_GC
Cuts at: 0 274 364 655 996
Size: 274 90 291 341
Fragments arranged by size:
341 291 274 90
BsaAI yAC'GTr
Cuts at: 0 200 681 996
Size: 200 481 315
Fragments arranged by size:
481 315 200
BsaHI Gr'CG_yC
Cuts at: 0 548 805 996
Size: 548 257 191
Fragments arranged by size:
548 257 191
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 804 834 880 910 996
Size: 804 30 46 30 86
Fragments arranged by size:
804 86 46 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 544 996
Size: 262 282 452
Fragments arranged by size:
452 282 262
BseYI C'CCAG_C
Cuts at: 0 102 299 495 794 996
Size: 102 197 196 299 202
Fragments arranged by size:
299 202 197 196 102
BsiEI CG_ry'CG
Cuts at: 0 10 996
Size: 10 986
BsiHKAI G_wGCw'C
Cuts at: 0 71 308 443 996
Size: 71 237 135 553
Fragments arranged by size:
553 237 135 71
BsmBI CGTCTCn'nnnn_
Cuts at: 0 796 996
Size: 796 200
Bsp1286I G_dGCh'C
Cuts at: 0 18 59 71 109 308 316 370 443
Size: 18 41 12 38 199 8 54 73
Cuts at: 443 505 544 996
Size: 62 39 452
Fragments arranged by size:
452 199 73 62 54 41 39 38
18 12 8
BsrFI r'CCGG_y
Cuts at: 0 109 996
Size: 109 887
BssSI C'ACGA_G
Cuts at: 0 307 433 996
Size: 307 126 563
Fragments arranged by size:
563 307 126
BstAPI GCAn_nnn'nTGC
Cuts at: 0 462 996
Size: 462 534
BstXI CCAn_nnnn'nTGG
Cuts at: 0 291 378 495 996
Size: 291 87 117 501
Fragments arranged by size:
501 291 117 87
BstYI r'GATC_y
Cuts at: 0 419 513 743 996
Size: 419 94 230 253
Fragments arranged by size:
419 253 230 94
BtgI C'CryG_G
Cuts at: 0 10 662 935 996
Size: 10 652 273 61
Fragments arranged by size:
652 273 61 10
EarI CTCTTCn'nnn_
Cuts at: 0 397 577 601 996
Size: 397 180 24 395
Fragments arranged by size:
397 395 180 24
EcoICRI GAG'CTC
Cuts at: 0 306 996
Size: 306 690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 148 636 820 888 996
Size: 135 13 488 184 68 108
Fragments arranged by size:
488 184 135 108 68 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 702 996
Size: 702 294
EcoO109I rG'GnC_Cy
Cuts at: 0 877 931 996
Size: 877 54 65
Fragments arranged by size:
877 65 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 867 996
Size: 867 129
HaeII r_GCGC'y
Cuts at: 0 103 220 300 551 996
Size: 103 117 80 251 445
Fragments arranged by size:
445 251 117 103 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 841 873 880 912 996
Size: 841 32 7 32 84
Fragments arranged by size:
841 84 32 32 7
KasI G'GCGC_C
Cuts at: 0 547 996
Size: 547 449
KpnI G_GTAC'C
Cuts at: 0 711 996
Size: 711 285
MslI CAynn'nnrTG
Cuts at: 0 112 289 996
Size: 112 177 707
Fragments arranged by size:
707 177 112
MspA1I CmG'CkG
Cuts at: 0 12 591 798 996
Size: 12 579 207 198
Fragments arranged by size:
579 207 198 12
NarI GG'CG_CC
Cuts at: 0 548 996
Size: 548 448
NcoI C'CATG_G
Cuts at: 0 935 996
Size: 935 61
PfoI T'CCnGG_A
Cuts at: 0 233 996
Size: 233 763
PmlI CAC'GTG
Cuts at: 0 200 996
Size: 200 796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 804 836 996
Size: 804 32 160
Fragments arranged by size:
804 160 32
PpuMI rG'GwC_Cy
Cuts at: 0 877 931 996
Size: 877 54 65
Fragments arranged by size:
877 65 54
PspOMI G'GGCC_C
Cuts at: 0 14 996
Size: 14 982
PstI C_TGCA'G
Cuts at: 0 978 996
Size: 978 18
PvuII CAG'CTG
Cuts at: 0 798 996
Size: 798 198
SacI G_AGCT'C
Cuts at: 0 308 996
Size: 308 688
SacII CC_GC'GG
Cuts at: 0 13 996
Size: 13 983
SanDI GG'GwC_CC
Cuts at: 0 931 996
Size: 931 65
SapI GCTCTTCn'nnn_
Cuts at: 0 577 601 996
Size: 577 24 395
Fragments arranged by size:
577 395 24
SfcI C'TryA_G
Cuts at: 0 974 996
Size: 974 22
SfoI GGC'GCC
Cuts at: 0 549 996
Size: 549 447
SgrAI Cr'CCGG_yG
Cuts at: 0 109 996
Size: 109 887
SmaI CCC'GGG
Cuts at: 0 19 996
Size: 19 977
SrfI GCCC'GGGC
Cuts at: 0 19 996
Size: 19 977
StyI C'CwwG_G
Cuts at: 0 935 996
Size: 935 61
XmaI C'CCGG_G
Cuts at: 0 17 996
Size: 17 979
Enzymes that do cut:
Acc65I AflIII AhdI AleI AloI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BclI BglI
Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BssSI
BstAPI BstXI BstYI BtgI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI KpnI MslI MspA1I
NarI NcoI PfoI PmlI PpiI PpuMI PspOMI PstI
PvuII SacI SacII SanDI SapI SfcI SfoI SgrAI
SmaI SrfI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BfrBI BglII BlpI BmgBI BmtI BpuEI
BsaI BsaBI BsaWI BsgI BsiWI BsmI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI MfeI MluI MmeI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SbfI ScaI SexAI SfiI SgfI
SmlI SnaBI SpeI SphI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI