(Linear) (Six-base) MAPSORT of: KIEE5404.seq  Check: 7051  from: 1  to: 930



KIEE5404.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:58  ..

AatII G_ACGT'C

Cuts at:      0      11     930 
   Size:         11     919
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     928     930 
   Size:        928       2
 
AlwNI CAG_nnn'CTG

Cuts at:      0     675     930 
   Size:        675     255
 
ApoI r'AATT_y

Cuts at:      0     377     930 
   Size:        377     553
 
BanI G'GyrC_C

Cuts at:      0     865     930 
   Size:        865      65
 
BclI T'GATC_A

Cuts at:      0     155     930 
   Size:        155     775
 
BglI GCCn_nnn'nGGC

Cuts at:      0      61     930 
   Size:         61     869
 
BmtI G_CTAG'C

Cuts at:      0     205     930 
   Size:        205     725
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     182     930 
   Size:         66     116     748

  Fragments arranged by size: 

                 748     116      66
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     538     930 
   Size:        538     392
 
BsaHI Gr'CG_yC

Cuts at:      0       8     930 
   Size:          8     922
 
BsaWI w'CCGG_w

Cuts at:      0     438     930 
   Size:        438     492
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     460     490     930 
   Size:        460      30     440

  Fragments arranged by size: 

                 460     440      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     306     930 
   Size:        306     624
 
BsmI GAATG_Cn'

Cuts at:      0     404     930 
   Size:        404     526
 
BsrBI CCG'CTC

Cuts at:      0     279     930 
   Size:        279     651
 
BsrDI GCAATG_nn'

Cuts at:      0     260     707     930 
   Size:        260     447     223

  Fragments arranged by size: 

                 447     260     223
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     185     930 
   Size:        185     745
 
BtsI GCAGTG_nn'

Cuts at:      0     158     930 
   Size:        158     772
 
DraIII CAC_nnn'GTG

Cuts at:      0     897     930 
   Size:        897      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     182     928     930 
   Size:         66     116     746       2

  Fragments arranged by size: 

                 746     116      66       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     637     873     930 
   Size:        637     236      57

  Fragments arranged by size: 

                 637     236      57
 
EcoO109I rG'GnC_Cy

Cuts at:      0     630     930 
   Size:        630     300
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     144     373     930 
   Size:        144     229     557

  Fragments arranged by size: 

                 557     229     144
 
FspI TGC'GCA

Cuts at:      0     618     930 
   Size:        618     312
 
FspAI rTGC'GCAy

Cuts at:      0     618     930 
   Size:        618     312
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     696     728     930 
   Size:        696      32     202

  Fragments arranged by size: 

                 696     202      32
 
HincII GTy'rAC

Cuts at:      0     387     930 
   Size:        387     543
 
MfeI C'AATT_G

Cuts at:      0     779     930 
   Size:        779     151
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     375     930 
   Size:        375     555
 
MslI CAynn'nnrTG

Cuts at:      0     670     930 
   Size:        670     260
 
MspA1I CmG'CkG

Cuts at:      0     863     930 
   Size:        863      67
 
NdeI CA'TA_TG

Cuts at:      0     512     684     930 
   Size:        512     172     246

  Fragments arranged by size: 

                 512     246     172
 
NheI G'CTAG_C

Cuts at:      0     201     930 
   Size:        201     729
 
PfoI T'CCnGG_A

Cuts at:      0      11     930 
   Size:         11     919
 
SfcI C'TryA_G

Cuts at:      0     284     858     930 
   Size:        284     574      72

  Fragments arranged by size: 

                 574     284      72
 
StyI C'CwwG_G

Cuts at:      0     571     602     626     920     930 
   Size:        571      31      24     294      10

  Fragments arranged by size: 

                 571     294      31      24      10
 
TatI w'GTAC_w

Cuts at:      0     590     930 
   Size:        590     340
 
ZraI GAC'GTC

Cuts at:      0       9     930 
   Size:          9     921
 

 Enzymes that do cut:

    AatII     AcuI    AlwNI     ApoI     BanI     BclI     BglI     BmtI
     BpmI     BsaI    BsaHI    BsaWI    BsaXI    BseRI     BsmI    BsrBI
    BsrDI    BtgZI     BtsI   DraIII  Eco57MI    EcoNI EcoO109I     FalI
     FspI    FspAI    Hin4I   HincII     MfeI     MmeI     MslI   MspA1I
     NdeI     NheI     PfoI     SfcI     StyI     TatI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FseI
    HaeII  HindIII     HpaI     KasI     KpnI     MluI     MscI     NaeI
     NarI     NcoI   NgoMIV     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI