Restriction map for
pFN21AE5402
(Linear) (Six-base) MAPSORT of: KIEE5402.seq Check: 2316 from: 1 to: 939
KIEE5402.orf
With 182 enzymes: SgfI *
January 29, 2014 11:58 ..
AccI GT'mk_AC
Cuts at: 0 371 939
Size: 371 568
AfeI AGC'GCT
Cuts at: 0 397 939
Size: 397 542
AflIII A'CryG_T
Cuts at: 0 680 845 939
Size: 680 165 94
Fragments arranged by size:
680 165 94
AlwNI CAG_nnn'CTG
Cuts at: 0 289 456 879 939
Size: 289 167 423 60
Fragments arranged by size:
423 289 167 60
ApaI G_GGCC'C
Cuts at: 0 596 939
Size: 596 343
ApoI r'AATT_y
Cuts at: 0 698 939
Size: 698 241
BamHI G'GATC_C
Cuts at: 0 341 927 939
Size: 341 586 12
Fragments arranged by size:
586 341 12
BanII G_rGCy'C
Cuts at: 0 596 939
Size: 596 343
BbsI GAAGACnn'nnnn_
Cuts at: 0 495 898 939
Size: 495 403 41
Fragments arranged by size:
495 403 41
BciVI GTATCCnnnnn_n'
Cuts at: 0 579 939
Size: 579 360
BclI T'GATC_A
Cuts at: 0 140 939
Size: 140 799
BfrBI ATG'CAT
Cuts at: 0 463 786 939
Size: 463 323 153
Fragments arranged by size:
463 323 153
BglII A'GATC_T
Cuts at: 0 306 939
Size: 306 633
Bme1580I G_kGCm'C
Cuts at: 0 596 939
Size: 596 343
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 417 939
Size: 417 522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 223 271 939
Size: 51 172 48 668
Fragments arranged by size:
668 172 51 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 939
Size: 302 637
BsaAI yAC'GTr
Cuts at: 0 410 939
Size: 410 529
BsaWI w'CCGG_w
Cuts at: 0 422 632 939
Size: 422 210 307
Fragments arranged by size:
422 307 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 939
Size: 610 329
Bsp1286I G_dGCh'C
Cuts at: 0 596 939
Size: 596 343
BspEI T'CCGG_A
Cuts at: 0 422 632 939
Size: 422 210 307
Fragments arranged by size:
422 307 210
BsrBI CCG'CTC
Cuts at: 0 933 939
Size: 933 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 610 804 939
Size: 610 194 135
Fragments arranged by size:
610 194 135
BstXI CCAn_nnnn'nTGG
Cuts at: 0 712 939
Size: 712 227
BstYI r'GATC_y
Cuts at: 0 306 341 927 939
Size: 306 35 586 12
Fragments arranged by size:
586 306 35 12
Bsu36I CC'TnA_GG
Cuts at: 0 449 939
Size: 449 490
BtgI C'CryG_G
Cuts at: 0 581 939
Size: 581 358
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 623 939
Size: 623 316
BtsI GCAGTG_nn'
Cuts at: 0 143 939
Size: 143 796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 223 271 939
Size: 51 172 48 668
Fragments arranged by size:
668 172 51 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 49 807 939
Size: 49 758 132
Fragments arranged by size:
758 132 49
EcoO109I rG'GnC_Cy
Cuts at: 0 592 593 812 939
Size: 592 1 219 127
Fragments arranged by size:
592 219 127 1
HaeII r_GCGC'y
Cuts at: 0 399 939
Size: 399 540
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 896 928 939
Size: 271 32 593 32 11
Fragments arranged by size:
593 271 32 32 11
HindIII A'AGCT_T
Cuts at: 0 619 939
Size: 619 320
MslI CAynn'nnrTG
Cuts at: 0 442 572 939
Size: 442 130 367
Fragments arranged by size:
442 367 130
MspA1I CmG'CkG
Cuts at: 0 876 939
Size: 876 63
NcoI C'CATG_G
Cuts at: 0 581 939
Size: 581 358
NsiI A_TGCA'T
Cuts at: 0 465 788 939
Size: 465 323 151
Fragments arranged by size:
465 323 151
NspI r_CATG'y
Cuts at: 0 684 849 939
Size: 684 165 90
Fragments arranged by size:
684 165 90
PasI CC'CwG_GG
Cuts at: 0 741 939
Size: 741 198
PciI A'CATG_T
Cuts at: 0 680 845 939
Size: 680 165 94
Fragments arranged by size:
680 165 94
PflMI CCAn_nnn'nTGG
Cuts at: 0 879 939
Size: 879 60
PfoI T'CCnGG_A
Cuts at: 0 185 939
Size: 185 754
PpuMI rG'GwC_Cy
Cuts at: 0 812 939
Size: 812 127
PspOMI G'GGCC_C
Cuts at: 0 592 939
Size: 592 347
PvuII CAG'CTG
Cuts at: 0 876 939
Size: 876 63
ScaI AGT'ACT
Cuts at: 0 217 939
Size: 217 722
SmlI C'TyrA_G
Cuts at: 0 317 939
Size: 317 622
StyI C'CwwG_G
Cuts at: 0 581 711 900 939
Size: 581 130 189 39
Fragments arranged by size:
581 189 130 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 373 754 939
Size: 373 381 185
Fragments arranged by size:
381 373 185
TatI w'GTAC_w
Cuts at: 0 215 939
Size: 215 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 190 222 939
Size: 190 32 717
Fragments arranged by size:
717 190 32
XmnI GAAnn'nnTTC
Cuts at: 0 179 502 939
Size: 179 323 437
Fragments arranged by size:
437 323 179
Enzymes that do cut:
AccI AfeI AflIII AlwNI ApaI ApoI BamHI BanII
BbsI BciVI BclI BfrBI BglII Bme1580I BplI BpmI
BpuEI BsaAI BsaWI BsgI Bsp1286I BspEI BsrBI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI Eco57MI EcoNI
EcoO109I HaeII Hin4I HindIII MslI MspA1I NcoI NsiI
NspI PasI PciI PflMI PfoI PpuMI PspOMI PvuII
ScaI SmlI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbvCI BcgI BglI BlpI
BmgBI BmrI BmtI Bpu10I BsaI BsaBI BsaHI BsaXI
BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI ZraI