Restriction map for
pFN21AE5392
(Linear) (Six-base) MAPSORT of: KIEE5392.seq Check: 9179 from: 1 to: 927
KIEE5392.orf
With 182 enzymes: SgfI *
January 29, 2014 11:58 ..
AccI GT'mk_AC
Cuts at: 0 206 927
Size: 206 721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 927
Size: 897 30
AfeI AGC'GCT
Cuts at: 0 611 927
Size: 611 316
AgeI A'CCGG_T
Cuts at: 0 362 927
Size: 362 565
AleI CACnn'nnGTG
Cuts at: 0 809 927
Size: 809 118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 927
Size: 416 32 479
Fragments arranged by size:
479 416 32
AlwNI CAG_nnn'CTG
Cuts at: 0 814 927
Size: 814 113
ApoI r'AATT_y
Cuts at: 0 31 47 462 927
Size: 31 16 415 465
Fragments arranged by size:
465 415 31 16
AseI AT'TA_AT
Cuts at: 0 542 927
Size: 542 385
AvaI C'yCGr_G
Cuts at: 0 118 927
Size: 118 809
BamHI G'GATC_C
Cuts at: 0 638 927
Size: 638 289
BmrI ACTGGGnnnn_n'
Cuts at: 0 644 927
Size: 644 283
Bpu10I CC'TnA_GC
Cuts at: 0 692 765 927
Size: 692 73 162
Fragments arranged by size:
692 162 73
BsaAI yAC'GTr
Cuts at: 0 676 927
Size: 676 251
BsaWI w'CCGG_w
Cuts at: 0 362 927
Size: 362 565
BseYI C'CCAG_C
Cuts at: 0 615 927
Size: 615 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 927
Size: 373 554
BsiHKAI G_wGCw'C
Cuts at: 0 119 927
Size: 119 808
Bsp1286I G_dGCh'C
Cuts at: 0 119 927
Size: 119 808
BspHI T'CATG_A
Cuts at: 0 449 927
Size: 449 478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 927
Size: 134 793
BsrFI r'CCGG_y
Cuts at: 0 362 927
Size: 362 565
BsrGI T'GTAC_A
Cuts at: 0 505 830 927
Size: 505 325 97
Fragments arranged by size:
505 325 97
BstEII G'GTnAC_C
Cuts at: 0 840 927
Size: 840 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 927
Size: 105 822
BstYI r'GATC_y
Cuts at: 0 638 927
Size: 638 289
BtsI GCAGTG_nn'
Cuts at: 0 493 927
Size: 493 434
EaeI y'GGCC_r
Cuts at: 0 140 371 927
Size: 140 231 556
Fragments arranged by size:
556 231 140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 927
Size: 897 30
EcoRI G'AATT_C
Cuts at: 0 462 927
Size: 462 465
HaeII r_GCGC'y
Cuts at: 0 613 927
Size: 613 314
HindIII A'AGCT_T
Cuts at: 0 477 927
Size: 477 450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 927
Size: 215 712
MscI TGG'CCA
Cuts at: 0 142 373 927
Size: 142 231 554
Fragments arranged by size:
554 231 142
MslI CAynn'nnrTG
Cuts at: 0 172 298 592 809 847 927
Size: 172 126 294 217 38 80
Fragments arranged by size:
294 217 172 126 80 38
NspI r_CATG'y
Cuts at: 0 764 927
Size: 764 163
PfoI T'CCnGG_A
Cuts at: 0 442 927
Size: 442 485
PstI C_TGCA'G
Cuts at: 0 819 927
Size: 819 108
SexAI A'CCwGG_T
Cuts at: 0 104 927
Size: 104 823
SfcI C'TryA_G
Cuts at: 0 815 867 927
Size: 815 52 60
Fragments arranged by size:
815 60 52
StuI AGG'CCT
Cuts at: 0 73 927
Size: 73 854
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 310 808 927
Size: 310 498 119
Fragments arranged by size:
498 310 119
TatI w'GTAC_w
Cuts at: 0 176 505 644 830 927
Size: 176 329 139 186 97
Fragments arranged by size:
329 186 176 139 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 438 470 927
Size: 438 32 457
Fragments arranged by size:
457 438 32
XmnI GAAnn'nnTTC
Cuts at: 0 35 859 907 927
Size: 35 824 48 20
Fragments arranged by size:
824 48 35 20
Enzymes that do cut:
AccI AcuI AfeI AgeI AleI AloI AlwNI ApoI
AseI AvaI BamHI BmrI Bpu10I BsaAI BsaWI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrFI BsrGI BstEII
BstXI BstYI BtsI EaeI Eco57MI EcoRI HaeII HindIII
MmeI MscI MslI NspI PfoI PstI SexAI SfcI
StuI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AhdI AlfI
ApaI ApaLI AscI AsiSI AvrII BaeI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI BpmI BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspI FspAI Hin4I HincII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI