Restriction map for
pFN21AE5390
(Linear) (Six-base) MAPSORT of: KIEE5390.seq Check: 5067 from: 1 to: 924
KIEE5390.orf
With 182 enzymes: SgfI *
January 29, 2014 11:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 924
Size: 727 197
AccI GT'mk_AC
Cuts at: 0 769 775 924
Size: 769 6 149
Fragments arranged by size:
769 149 6
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 924
Size: 713 211
AleI CACnn'nnGTG
Cuts at: 0 229 924
Size: 229 695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 403 924
Size: 403 521
ApoI r'AATT_y
Cuts at: 0 331 924
Size: 331 593
BanI G'GyrC_C
Cuts at: 0 540 895 924
Size: 540 355 29
Fragments arranged by size:
540 355 29
BanII G_rGCy'C
Cuts at: 0 50 350 924
Size: 50 300 574
Fragments arranged by size:
574 300 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 810 924
Size: 93 717 114
Fragments arranged by size:
717 114 93
BbvCI CC'TCA_GC
Cuts at: 0 187 553 924
Size: 187 366 371
Fragments arranged by size:
371 366 187
Bme1580I G_kGCm'C
Cuts at: 0 236 924
Size: 236 688
Bpu10I CC'TnA_GC
Cuts at: 0 69 187 553 924
Size: 69 118 366 371
Fragments arranged by size:
371 366 118 69
BsaAI yAC'GTr
Cuts at: 0 369 773 924
Size: 369 404 151
Fragments arranged by size:
404 369 151
BsaBI GATnn'nnATC
Cuts at: 0 664 924
Size: 664 260
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 175 463 924
Size: 175 288 461
Fragments arranged by size:
461 288 175
BseYI C'CCAG_C
Cuts at: 0 342 924
Size: 342 582
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 924
Size: 273 651
Bsp1286I G_dGCh'C
Cuts at: 0 50 236 350 924
Size: 50 186 114 574
Fragments arranged by size:
574 186 114 50
BspHI T'CATG_A
Cuts at: 0 131 924
Size: 131 793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 727 924
Size: 727 197
BsrDI GCAATG_nn'
Cuts at: 0 620 924
Size: 620 304
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 924
Size: 243 681
BstZ17I GTA'TAC
Cuts at: 0 770 776 924
Size: 770 6 148
Fragments arranged by size:
770 148 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 267 924
Size: 267 657
BtsI GCAGTG_nn'
Cuts at: 0 290 385 924
Size: 290 95 539
Fragments arranged by size:
539 290 95
EaeI y'GGCC_r
Cuts at: 0 110 371 434 924
Size: 110 261 63 490
Fragments arranged by size:
490 261 110 63
EarI CTCTTCn'nnn_
Cuts at: 0 55 729 898 924
Size: 55 674 169 26
Fragments arranged by size:
674 169 55 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 924
Size: 713 211
EcoNI CCTnn'n_nnAGG
Cuts at: 0 654 924
Size: 654 270
EcoRI G'AATT_C
Cuts at: 0 331 924
Size: 331 593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 726 924
Size: 726 198
MscI TGG'CCA
Cuts at: 0 112 373 436 924
Size: 112 261 63 488
Fragments arranged by size:
488 261 112 63
MslI CAynn'nnrTG
Cuts at: 0 112 172 229 487 670 736 924
Size: 112 60 57 258 183 66 188
Fragments arranged by size:
258 188 183 112 66 60 57
MspA1I CmG'CkG
Cuts at: 0 410 924
Size: 410 514
PflMI CCAn_nnn'nTGG
Cuts at: 0 443 924
Size: 443 481
PvuII CAG'CTG
Cuts at: 0 410 924
Size: 410 514
SapI GCTCTTCn'nnn_
Cuts at: 0 55 729 924
Size: 55 674 195
Fragments arranged by size:
674 195 55
SfcI C'TryA_G
Cuts at: 0 867 924
Size: 867 57
SnaBI TAC'GTA
Cuts at: 0 773 924
Size: 773 151
StuI AGG'CCT
Cuts at: 0 709 924
Size: 709 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 558 883 924
Size: 558 325 41
Fragments arranged by size:
558 325 41
TatI w'GTAC_w
Cuts at: 0 176 924
Size: 176 748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 555 924
Size: 555 369
Enzymes that do cut:
AarI AccI AcuI AleI AlfI ApoI BanI BanII
BbsI BbvCI Bme1580I Bpu10I BsaAI BsaBI BseRI BseYI
BsgI Bsp1286I BspHI BspMI BsrDI BstXI BstZ17I BtgZI
BtsI EaeI EarI Eco57MI EcoNI EcoRI FalI MscI
MslI MspA1I PflMI PvuII SapI SfcI SnaBI StuI
TaqII TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRV
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI SspI
StyI SwaI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI