Restriction map for
pFN21AE5389
(Linear) (Six-base) MAPSORT of: KIEE5389.seq Check: 1125 from: 1 to: 924
KIEE5389.orf
With 182 enzymes: SgfI *
January 29, 2014 11:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 924
Size: 710 214
AleI CACnn'nnGTG
Cuts at: 0 22 924
Size: 22 902
AlwNI CAG_nnn'CTG
Cuts at: 0 157 924
Size: 157 767
ApoI r'AATT_y
Cuts at: 0 31 123 924
Size: 31 92 801
Fragments arranged by size:
801 92 31
BanII G_rGCy'C
Cuts at: 0 164 924
Size: 164 760
BfrBI ATG'CAT
Cuts at: 0 211 427 924
Size: 211 216 497
Fragments arranged by size:
497 216 211
BlpI GC'TnA_GC
Cuts at: 0 616 924
Size: 616 308
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 924
Size: 48 876
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 702 924
Size: 702 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 924
Size: 44 880
BsaI GGTCTCn'nnnn_
Cuts at: 0 603 924
Size: 603 321
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 675 924
Size: 331 344 249
Fragments arranged by size:
344 331 249
BseYI C'CCAG_C
Cuts at: 0 795 924
Size: 795 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 549 924
Size: 475 74 375
Fragments arranged by size:
475 375 74
BsiHKAI G_wGCw'C
Cuts at: 0 683 924
Size: 683 241
Bsp1286I G_dGCh'C
Cuts at: 0 164 683 924
Size: 164 519 241
Fragments arranged by size:
519 241 164
BspHI T'CATG_A
Cuts at: 0 404 924
Size: 404 520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 765 924
Size: 765 159
BsrBI CCG'CTC
Cuts at: 0 325 924
Size: 325 599
BsrFI r'CCGG_y
Cuts at: 0 380 924
Size: 380 544
BsrGI T'GTAC_A
Cuts at: 0 471 924
Size: 471 453
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 924
Size: 243 681
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 354 924
Size: 354 570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 924
Size: 710 214
EcoRI G'AATT_C
Cuts at: 0 31 924
Size: 31 893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 499 924
Size: 499 425
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 42 74 241 273 924
Size: 42 32 167 32 651
Fragments arranged by size:
651 167 42 32 32
HincII GTy'rAC
Cuts at: 0 910 924
Size: 910 14
HindIII A'AGCT_T
Cuts at: 0 884 924
Size: 884 40
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 924
Size: 626 298
MslI CAynn'nnrTG
Cuts at: 0 22 924
Size: 22 902
MspA1I CmG'CkG
Cuts at: 0 799 924
Size: 799 125
NaeI GCC'GGC
Cuts at: 0 382 924
Size: 382 542
NdeI CA'TA_TG
Cuts at: 0 914 924
Size: 914 10
NgoMIV G'CCGG_C
Cuts at: 0 380 924
Size: 380 544
NsiI A_TGCA'T
Cuts at: 0 213 429 924
Size: 213 216 495
Fragments arranged by size:
495 216 213
NspI r_CATG'y
Cuts at: 0 719 924
Size: 719 205
PasI CC'CwG_GG
Cuts at: 0 157 924
Size: 157 767
PvuII CAG'CTG
Cuts at: 0 799 924
Size: 799 125
SfcI C'TryA_G
Cuts at: 0 279 924
Size: 279 645
SmlI C'TyrA_G
Cuts at: 0 59 924
Size: 59 865
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 739 924
Size: 739 185
TatI w'GTAC_w
Cuts at: 0 471 924
Size: 471 453
Tth111I GACn'n_nGTC
Cuts at: 0 54 924
Size: 54 870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 243 576 924
Size: 243 333 348
Fragments arranged by size:
348 333 243
XmnI GAAnn'nnTTC
Cuts at: 0 35 924
Size: 35 889
Enzymes that do cut:
AcuI AleI AlwNI ApoI BanII BfrBI BlpI BmrI
BplI BpuEI BsaI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrGI BstXI BtgZI Eco57MI
EcoRI FalI Hin4I HincII HindIII MmeI MslI MspA1I
NaeI NdeI NgoMIV NsiI NspI PasI PvuII SfcI
SmlI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII Bme1580I BmgBI
BmtI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BsrDI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI MscI NarI
NcoI NheI NotI NruI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TstI XbaI XhoI XmaI ZraI