(Linear) (Six-base) MAPSORT of: KIEE5387.seq  Check: 593  from: 1  to: 924



KIEE5387.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     260     924 
   Size:        260     664
 
AccI GT'mk_AC

Cuts at:      0     101     473     924 
   Size:        101     372     451

  Fragments arranged by size: 

                 451     372     101
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     403     924 
   Size:        403     521
 
ApoI r'AATT_y

Cuts at:      0      31     915     924 
   Size:         31     884       9

  Fragments arranged by size: 

                 884      31       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     782     924 
   Size:        782     142
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     657     924 
   Size:        657     267
 
BfrBI ATG'CAT

Cuts at:      0     580     924 
   Size:        580     344
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     324     924 
   Size:        324     600
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     688     924 
   Size:        688     236
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     610     924 
   Size:        610     314
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     530     924 
   Size:        530     394
 
BsmI GAATG_Cn'

Cuts at:      0     277     455     924 
   Size:        277     178     469

  Fragments arranged by size: 

                 469     277     178
 
BspHI T'CATG_A

Cuts at:      0     404     924 
   Size:        404     520
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     260     924 
   Size:        260     664
 
BsrDI GCAATG_nn'

Cuts at:      0     272     518     924 
   Size:        272     246     406

  Fragments arranged by size: 

                 406     272     246
 
Bsu36I CC'TnA_GG

Cuts at:      0     236     924 
   Size:        236     688
 
BtsI GCAGTG_nn'

Cuts at:      0     353     924 
   Size:        353     571
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     786     924 
   Size:        786     138
 
EaeI y'GGCC_r

Cuts at:      0     590     654     924 
   Size:        590      64     270

  Fragments arranged by size: 

                 590     270      64
 
EcoO109I rG'GnC_Cy

Cuts at:      0     540     924 
   Size:        540     384
 
EcoRI G'AATT_C

Cuts at:      0      31     924 
   Size:         31     893
 
FspI TGC'GCA

Cuts at:      0     291     924 
   Size:        291     633
 
HincII GTy'rAC

Cuts at:      0     153     689     924 
   Size:        153     536     235

  Fragments arranged by size: 

                 536     235     153
 
MscI TGG'CCA

Cuts at:      0     592     924 
   Size:        592     332
 
MslI CAynn'nnrTG

Cuts at:      0     172     460     775     924 
   Size:        172     288     315     149

  Fragments arranged by size: 

                 315     288     172     149
 
MspA1I CmG'CkG

Cuts at:      0      75     429     924 
   Size:         75     354     495

  Fragments arranged by size: 

                 495     354      75
 
NsiI A_TGCA'T

Cuts at:      0     582     924 
   Size:        582     342
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     735     924 
   Size:        735     189
 
PvuII CAG'CTG

Cuts at:      0      75     429     924 
   Size:         75     354     495

  Fragments arranged by size: 

                 495     354      75
 
SexAI A'CCwGG_T

Cuts at:      0     104     924 
   Size:        104     820
 
SfcI C'TryA_G

Cuts at:      0     861     924 
   Size:        861      63
 
SmlI C'TyrA_G

Cuts at:      0     703     924 
   Size:        703     221
 
SpeI A'CTAG_T

Cuts at:      0     243     924 
   Size:        243     681
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      85     186     311     589     798     924 
   Size:         85     101     125     278     209     126

  Fragments arranged by size: 

                 278     209     126     125     101      85
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     241     273     405     437     924 
   Size:        241      32     132      32     487

  Fragments arranged by size: 

                 487     241     132      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     456     924 
   Size:        456     468
 

 Enzymes that do cut:

     AarI     AccI     AlfI     ApoI     BbsI    BciVI    BfrBI     BmrI
    BpuEI    BseRI     BsgI     BsmI    BspHI    BspMI    BsrDI   Bsu36I
     BtsI     DrdI     EaeI EcoO109I    EcoRI     FspI   HincII     MscI
     MslI   MspA1I     NsiI    PflMI    PvuII    SexAI     SfcI     SmlI
     SpeI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRV     FalI     FseI    FspAI    HaeII    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI