Restriction map for
pFN21AE5386
(Linear) (Six-base) MAPSORT of: KIEE5386.seq Check: 540 from: 1 to: 921
KIEE5386.orf
With 182 enzymes: SgfI *
January 29, 2014 11:58 ..
ApaI G_GGCC'C
Cuts at: 0 622 921
Size: 622 299
ApoI r'AATT_y
Cuts at: 0 25 921
Size: 25 896
BanII G_rGCy'C
Cuts at: 0 622 921
Size: 622 299
BbvCI CC'TCA_GC
Cuts at: 0 407 921
Size: 407 514
BciVI GTATCCnnnnn_n'
Cuts at: 0 523 921
Size: 523 398
BclI T'GATC_A
Cuts at: 0 908 921
Size: 908 13
BfrBI ATG'CAT
Cuts at: 0 284 921
Size: 284 637
BglI GCCn_nnn'nGGC
Cuts at: 0 346 921
Size: 346 575
Bme1580I G_kGCm'C
Cuts at: 0 622 921
Size: 622 299
BmrI ACTGGGnnnn_n'
Cuts at: 0 122 921
Size: 122 799
Bpu10I CC'TnA_GC
Cuts at: 0 407 921
Size: 407 514
BsaBI GATnn'nnATC
Cuts at: 0 139 427 921
Size: 139 288 494
Fragments arranged by size:
494 288 139
BsaWI w'CCGG_w
Cuts at: 0 809 921
Size: 809 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 316 346 921
Size: 316 30 575
Fragments arranged by size:
575 316 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 921
Size: 22 899
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 921
Size: 451 470
Bsp1286I G_dGCh'C
Cuts at: 0 622 921
Size: 622 299
BspHI T'CATG_A
Cuts at: 0 398 921
Size: 398 523
BsrDI GCAATG_nn'
Cuts at: 0 541 921
Size: 541 380
BsrGI T'GTAC_A
Cuts at: 0 827 921
Size: 827 94
BtgI C'CryG_G
Cuts at: 0 614 921
Size: 614 307
BtsI GCAGTG_nn'
Cuts at: 0 223 921
Size: 223 698
EaeI y'GGCC_r
Cuts at: 0 425 921
Size: 425 496
EarI CTCTTCn'nnn_
Cuts at: 0 79 673 921
Size: 79 594 248
Fragments arranged by size:
594 248 79
EcoO109I rG'GnC_Cy
Cuts at: 0 619 893 921
Size: 619 274 28
Fragments arranged by size:
619 274 28
EcoRV GAT'ATC
Cuts at: 0 204 921
Size: 204 717
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 912 921
Size: 912 9
MfeI C'AATT_G
Cuts at: 0 608 921
Size: 608 313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 921
Size: 530 391
MscI TGG'CCA
Cuts at: 0 427 921
Size: 427 494
MslI CAynn'nnrTG
Cuts at: 0 106 921
Size: 106 815
NcoI C'CATG_G
Cuts at: 0 614 921
Size: 614 307
NsiI A_TGCA'T
Cuts at: 0 286 921
Size: 286 635
NspI r_CATG'y
Cuts at: 0 696 921
Size: 696 225
PflMI CCAn_nnn'nTGG
Cuts at: 0 312 732 921
Size: 312 420 189
Fragments arranged by size:
420 312 189
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 921
Size: 235 32 654
Fragments arranged by size:
654 235 32
PspOMI G'GGCC_C
Cuts at: 0 618 921
Size: 618 303
PstI C_TGCA'G
Cuts at: 0 65 242 921
Size: 65 177 679
Fragments arranged by size:
679 177 65
ScaI AGT'ACT
Cuts at: 0 594 921
Size: 594 327
SfcI C'TryA_G
Cuts at: 0 61 238 921
Size: 61 177 683
Fragments arranged by size:
683 177 61
SphI G_CATG'C
Cuts at: 0 696 921
Size: 696 225
StyI C'CwwG_G
Cuts at: 0 614 896 921
Size: 614 282 25
Fragments arranged by size:
614 282 25
TatI w'GTAC_w
Cuts at: 0 592 827 846 921
Size: 592 235 19 75
Fragments arranged by size:
592 235 75 19
Enzymes that do cut:
ApaI ApoI BanII BbvCI BciVI BclI BfrBI BglI
Bme1580I BmrI Bpu10I BsaBI BsaWI BsaXI BseRI BsgI
Bsp1286I BspHI BsrDI BsrGI BtgI BtsI EaeI EarI
EcoO109I EcoRV FalI MfeI MmeI MscI MslI NcoI
NsiI NspI PflMI PpiI PspOMI PstI ScaI SfcI
SphI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BcgI BglII BlpI BmgBI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaHI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI Eco57MI EcoNI EcoRI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI