(Linear) (Six-base) MAPSORT of: KIEE5384.seq  Check: 1755  from: 1  to: 921



KIEE5384.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:01  ..

AatII G_ACGT'C

Cuts at:      0     780     921 
   Size:        780     141
 
AccI GT'mk_AC

Cuts at:      0      95     921 
   Size:         95     826
 
AflIII A'CryG_T

Cuts at:      0     712     921 
   Size:        712     209
 
AleI CACnn'nnGTG

Cuts at:      0      16     109     921 
   Size:         16      93     812

  Fragments arranged by size: 

                 812      93      16
 
ApoI r'AATT_y

Cuts at:      0     326     921 
   Size:        326     595
 
BbvCI CC'TCA_GC

Cuts at:      0     152     921 
   Size:        152     769
 
BclI T'GATC_A

Cuts at:      0     590     905     921 
   Size:        590     315      16

  Fragments arranged by size: 

                 590     315      16
 
BlpI GC'TnA_GC

Cuts at:      0     681     921 
   Size:        681     240
 
Bpu10I CC'TnA_GC

Cuts at:      0     152     921 
   Size:        152     769
 
BsaHI Gr'CG_yC

Cuts at:      0     777     921 
   Size:        777     144
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     802     921 
   Size:        802     119
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     551     921 
   Size:        551     370
 
BsmI GAATG_Cn'

Cuts at:      0     680     921 
   Size:        680     241
 
BsrDI GCAATG_nn'

Cuts at:      0     305     659     921 
   Size:        305     354     262

  Fragments arranged by size: 

                 354     305     262
 
BsrFI r'CCGG_y

Cuts at:      0     601     921 
   Size:        601     320
 
BstYI r'GATC_y

Cuts at:      0     534     921 
   Size:        534     387
 
BtgI C'CryG_G

Cuts at:      0     107     921 
   Size:        107     814
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     358     921 
   Size:        358     563
 
EarI CTCTTCn'nnn_

Cuts at:      0      76     921 
   Size:         76     845
 
EcoO109I rG'GnC_Cy

Cuts at:      0     566     719     921 
   Size:        566     153     202

  Fragments arranged by size: 

                 566     202     153
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     849     881     921 
   Size:        849      32      40

  Fragments arranged by size: 

                 849      40      32
 
MfeI C'AATT_G

Cuts at:      0     368     921 
   Size:        368     553
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     921 
   Size:        218     703
 
MslI CAynn'nnrTG

Cuts at:      0      16     106     109     130     550     921 
   Size:         16      90       3      21     420     371

  Fragments arranged by size: 

                 420     371      90      21      16       3
 
MspA1I CmG'CkG

Cuts at:      0     733     921 
   Size:        733     188
 
NaeI GCC'GGC

Cuts at:      0     603     921 
   Size:        603     318
 
NcoI C'CATG_G

Cuts at:      0     107     921 
   Size:        107     814
 
NgoMIV G'CCGG_C

Cuts at:      0     601     921 
   Size:        601     320
 
NspI r_CATG'y

Cuts at:      0     716     921 
   Size:        716     205
 
PciI A'CATG_T

Cuts at:      0     712     921 
   Size:        712     209
 
PpuMI rG'GwC_Cy

Cuts at:      0     566     719     921 
   Size:        566     153     202

  Fragments arranged by size: 

                 566     202     153
 
PshAI GACnn'nnGTC

Cuts at:      0     733     921 
   Size:        733     188
 
PvuII CAG'CTG

Cuts at:      0     733     921 
   Size:        733     188
 
SanDI GG'GwC_CC

Cuts at:      0     719     921 
   Size:        719     202
 
SfcI C'TryA_G

Cuts at:      0     861     921 
   Size:        861      60
 
StuI AGG'CCT

Cuts at:      0     703     921 
   Size:        703     218
 
StyI C'CwwG_G

Cuts at:      0     107     921 
   Size:        107     814
 
TatI w'GTAC_w

Cuts at:      0     170     288     491     921 
   Size:        170     118     203     430

  Fragments arranged by size: 

                 430     203     170     118
 
ZraI GAC'GTC

Cuts at:      0     778     921 
   Size:        778     143
 

 Enzymes that do cut:

    AatII     AccI   AflIII     AleI     ApoI    BbvCI     BclI     BlpI
   Bpu10I    BsaHI    BseRI     BsgI     BsmI    BsrDI    BsrFI    BstYI
     BtgI    BtgZI     EarI EcoO109I    Hin4I     MfeI     MmeI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NspI     PciI    PpuMI    PshAI
    PvuII    SanDI     SfcI     StuI     StyI     TatI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MscI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI