Restriction map for
pFN21AE5379
(Linear) (Six-base) MAPSORT of: KIEE5379.seq Check: 5673 from: 1 to: 915
KIEE5379.orf
With 182 enzymes: SgfI *
December 5, 2014 13:01 ..
AccI GT'mk_AC
Cuts at: 0 734 915
Size: 734 181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 843 890 915
Size: 843 47 25
Fragments arranged by size:
843 47 25
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 915
Size: 397 518
BclI T'GATC_A
Cuts at: 0 587 650 915
Size: 587 63 265
Fragments arranged by size:
587 265 63
BglII A'GATC_T
Cuts at: 0 203 915
Size: 203 712
BmrI ACTGGGnnnn_n'
Cuts at: 0 535 915
Size: 535 380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 915
Size: 568 347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 915
Size: 259 656
BsaI GGTCTCn'nnnn_
Cuts at: 0 424 915
Size: 424 491
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 412 903 915
Size: 79 333 491 12
Fragments arranged by size:
491 333 79 12
BseYI C'CCAG_C
Cuts at: 0 111 915
Size: 111 804
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 915
Size: 643 272
BsiHKAI G_wGCw'C
Cuts at: 0 158 915
Size: 158 757
BsmI GAATG_Cn'
Cuts at: 0 456 870 915
Size: 456 414 45
Fragments arranged by size:
456 414 45
Bsp1286I G_dGCh'C
Cuts at: 0 158 915
Size: 158 757
BspHI T'CATG_A
Cuts at: 0 398 915
Size: 398 517
BsrDI GCAATG_nn'
Cuts at: 0 123 915
Size: 123 792
BsrGI T'GTAC_A
Cuts at: 0 95 915
Size: 95 820
BssSI C'ACGA_G
Cuts at: 0 424 915
Size: 424 491
BstEII G'GTnAC_C
Cuts at: 0 132 915
Size: 132 783
BstXI CCAn_nnnn'nTGG
Cuts at: 0 313 513 915
Size: 313 200 402
Fragments arranged by size:
402 313 200
BstYI r'GATC_y
Cuts at: 0 203 915
Size: 203 712
DraIII CAC_nnn'GTG
Cuts at: 0 543 915
Size: 543 372
EaeI y'GGCC_r
Cuts at: 0 143 365 392 915
Size: 143 222 27 523
Fragments arranged by size:
523 222 143 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 843 890 915
Size: 568 275 47 25
Fragments arranged by size:
568 275 47 25
EcoO109I rG'GnC_Cy
Cuts at: 0 447 699 773 915
Size: 447 252 74 142
Fragments arranged by size:
447 252 142 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 255 915
Size: 255 660
FspI TGC'GCA
Cuts at: 0 561 915
Size: 561 354
HincII GTy'rAC
Cuts at: 0 735 915
Size: 735 180
HindIII A'AGCT_T
Cuts at: 0 232 915
Size: 232 683
MscI TGG'CCA
Cuts at: 0 145 367 394 915
Size: 145 222 27 521
Fragments arranged by size:
521 222 145 27
MslI CAynn'nnrTG
Cuts at: 0 76 130 292 915
Size: 76 54 162 623
Fragments arranged by size:
623 162 76 54
MspA1I CmG'CkG
Cuts at: 0 103 915
Size: 103 812
PflMI CCAn_nnn'nTGG
Cuts at: 0 681 915
Size: 681 234
PstI C_TGCA'G
Cuts at: 0 795 915
Size: 795 120
PvuII CAG'CTG
Cuts at: 0 103 915
Size: 103 812
SalI G'TCGA_C
Cuts at: 0 733 915
Size: 733 182
SfcI C'TryA_G
Cuts at: 0 791 915
Size: 791 124
SmlI C'TyrA_G
Cuts at: 0 274 915
Size: 274 641
StuI AGG'CCT
Cuts at: 0 328 915
Size: 328 587
StyI C'CwwG_G
Cuts at: 0 758 915
Size: 758 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 554 915
Size: 554 361
TatI w'GTAC_w
Cuts at: 0 95 915
Size: 95 820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 313 412 915
Size: 313 99 503
Fragments arranged by size:
503 313 99
Enzymes that do cut:
AccI AcuI AlfI BclI BglII BmrI BpmI BpuEI
BsaI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BsrDI BsrGI BssSI BstEII BstXI BstYI DraIII EaeI
Eco57MI EcoO109I FalI FspI HincII HindIII MscI MslI
MspA1I PflMI PstI PvuII SalI SfcI SmlI StuI
StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BlpI
Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspMI BsrBI
BsrFI BssHII BstAPI BstBI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspAI HaeII Hin4I
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI