(Linear) (Six-base) MAPSORT of: KIEE5377.seq  Check: 5388  from: 1  to: 912



KIEE5377.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:57  ..

Acc65I G'GTAC_C

Cuts at:      0     677     912 
   Size:        677     235
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     768     912 
   Size:         52     716     144

  Fragments arranged by size: 

                 716     144      52
 
AleI CACnn'nnGTG

Cuts at:      0     736     912 
   Size:        736     176
 
AlwNI CAG_nnn'CTG

Cuts at:      0     591     912 
   Size:        591     321
 
ApaLI G'TGCA_C

Cuts at:      0     460     912 
   Size:        460     452
 
ApoI r'AATT_y

Cuts at:      0      31     806     912 
   Size:         31     775     106

  Fragments arranged by size: 

                 775     106      31
 
BanI G'GyrC_C

Cuts at:      0     677     912 
   Size:        677     235
 
BanII G_rGCy'C

Cuts at:      0     412     614     912 
   Size:        412     202     298

  Fragments arranged by size: 

                 412     298     202
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      93     912 
   Size:         93     819
 
BlpI GC'TnA_GC

Cuts at:      0     187     912 
   Size:        187     725
 
Bme1580I G_kGCm'C

Cuts at:      0     464     912 
   Size:        464     448
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     434     912 
   Size:        434     478
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     625     912 
   Size:        625     287
 
BsiHKAI G_wGCw'C

Cuts at:      0     422     464     912 
   Size:        422      42     448

  Fragments arranged by size: 

                 448     422      42
 
Bsp1286I G_dGCh'C

Cuts at:      0     412     422     464     614     912 
   Size:        412      10      42     150     298

  Fragments arranged by size: 

                 412     298     150      42      10
 
BspHI T'CATG_A

Cuts at:      0     404     912 
   Size:        404     508
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     134     912 
   Size:        134     778
 
BsrDI GCAATG_nn'

Cuts at:      0     596     912 
   Size:        596     316
 
BtgI C'CryG_G

Cuts at:      0     350     412     912 
   Size:        350      62     500

  Fragments arranged by size: 

                 500     350      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     768     912 
   Size:         52     716     144

  Fragments arranged by size: 

                 716     144      52
 
HincII GTy'rAC

Cuts at:      0     491     912 
   Size:        491     421
 
KpnI G_GTAC'C

Cuts at:      0     681     912 
   Size:        681     231
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     432     912 
   Size:        432     480
 
MslI CAynn'nnrTG

Cuts at:      0     136     172     208     736     912 
   Size:        136      36      36     528     176

  Fragments arranged by size: 

                 528     176     136      36      36
 
NcoI C'CATG_G

Cuts at:      0     350     912 
   Size:        350     562
 
NdeI CA'TA_TG

Cuts at:      0     376     912 
   Size:        376     536
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      21     278     912 
   Size:         21     257     634

  Fragments arranged by size: 

                 634     257      21
 
SfcI C'TryA_G

Cuts at:      0     583     912 
   Size:        583     329
 
SspI AAT'ATT

Cuts at:      0     665     912 
   Size:        665     247
 
StyI C'CwwG_G

Cuts at:      0     350     912 
   Size:        350     562
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     310     912 
   Size:        310     602
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      80     112     912 
   Size:         80      32     800

  Fragments arranged by size: 

                 800      80      32
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI    AlwNI    ApaLI     ApoI     BanI    BanII
     BbsI     BlpI Bme1580I     BmrI    BseRI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrDI     BtgI  Eco57MI   HincII     KpnI     MmeI     MslI
     NcoI     NdeI    PflMI     SfcI     SspI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     MfeI     MluI     MscI   MspA1I     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI