Restriction map for
pFN21AE5376
(Linear) (Six-base) MAPSORT of: KIEE5376.seq Check: 1182 from: 1 to: 909
KIEE5376.orf
With 182 enzymes: SgfI *
December 5, 2014 13:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 909
Size: 435 474
AflIII A'CryG_T
Cuts at: 0 679 909
Size: 679 230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 364 793 909
Size: 364 429 116
Fragments arranged by size:
429 364 116
AlwNI CAG_nnn'CTG
Cuts at: 0 899 909
Size: 899 10
BanI G'GyrC_C
Cuts at: 0 469 909
Size: 469 440
BbvCI CC'TCA_GC
Cuts at: 0 514 862 909
Size: 514 348 47
Fragments arranged by size:
514 348 47
BglI GCCn_nnn'nGGC
Cuts at: 0 391 909
Size: 391 518
BglII A'GATC_T
Cuts at: 0 548 909
Size: 548 361
Bme1580I G_kGCm'C
Cuts at: 0 197 909
Size: 197 712
BmrI ACTGGGnnnn_n'
Cuts at: 0 352 909
Size: 352 557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 909
Size: 187 722
Bpu10I CC'TnA_GC
Cuts at: 0 514 862 909
Size: 514 348 47
Fragments arranged by size:
514 348 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 909
Size: 622 287
BsaI GGTCTCn'nnnn_
Cuts at: 0 879 909
Size: 879 30
BseYI C'CCAG_C
Cuts at: 0 204 909
Size: 204 705
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 548 909
Size: 454 94 361
Fragments arranged by size:
454 361 94
BsmI GAATG_Cn'
Cuts at: 0 298 861 909
Size: 298 563 48
Fragments arranged by size:
563 298 48
Bsp1286I G_dGCh'C
Cuts at: 0 197 909
Size: 197 712
BspHI T'CATG_A
Cuts at: 0 365 581 909
Size: 365 216 328
Fragments arranged by size:
365 328 216
BsrDI GCAATG_nn'
Cuts at: 0 620 909
Size: 620 289
BssSI C'ACGA_G
Cuts at: 0 380 909
Size: 380 529
BstEII G'GTnAC_C
Cuts at: 0 69 102 909
Size: 69 33 807
Fragments arranged by size:
807 69 33
BstXI CCAn_nnnn'nTGG
Cuts at: 0 66 204 576 909
Size: 66 138 372 333
Fragments arranged by size:
372 333 138 66
BstYI r'GATC_y
Cuts at: 0 548 641 909
Size: 548 93 268
Fragments arranged by size:
548 268 93
Bsu36I CC'TnA_GG
Cuts at: 0 764 909
Size: 764 145
BtgI C'CryG_G
Cuts at: 0 311 909
Size: 311 598
EaeI y'GGCC_r
Cuts at: 0 332 909
Size: 332 577
EarI CTCTTCn'nnn_
Cuts at: 0 15 454 909
Size: 15 439 455
Fragments arranged by size:
455 439 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 435 909
Size: 187 248 474
Fragments arranged by size:
474 248 187
EcoO109I rG'GnC_Cy
Cuts at: 0 209 882 909
Size: 209 673 27
Fragments arranged by size:
673 209 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 543 909
Size: 543 366
MscI TGG'CCA
Cuts at: 0 334 909
Size: 334 575
MslI CAynn'nnrTG
Cuts at: 0 76 259 574 799 909
Size: 76 183 315 225 110
Fragments arranged by size:
315 225 183 110 76
NcoI C'CATG_G
Cuts at: 0 311 909
Size: 311 598
NdeI CA'TA_TG
Cuts at: 0 318 909
Size: 318 591
NspI r_CATG'y
Cuts at: 0 683 909
Size: 683 226
PciI A'CATG_T
Cuts at: 0 679 909
Size: 679 230
PflMI CCAn_nnn'nTGG
Cuts at: 0 466 909
Size: 466 443
PfoI T'CCnGG_A
Cuts at: 0 164 909
Size: 164 745
PpuMI rG'GwC_Cy
Cuts at: 0 209 909
Size: 209 700
PstI C_TGCA'G
Cuts at: 0 343 909
Size: 343 566
SanDI GG'GwC_CC
Cuts at: 0 209 909
Size: 209 700
SapI GCTCTTCn'nnn_
Cuts at: 0 454 909
Size: 454 455
SexAI A'CCwGG_T
Cuts at: 0 65 909
Size: 65 844
SfcI C'TryA_G
Cuts at: 0 339 422 828 909
Size: 339 83 406 81
Fragments arranged by size:
406 339 83 81
SmlI C'TyrA_G
Cuts at: 0 637 909
Size: 637 272
StyI C'CwwG_G
Cuts at: 0 243 311 909
Size: 243 68 598
Fragments arranged by size:
598 243 68
TatI w'GTAC_w
Cuts at: 0 137 909
Size: 137 772
XmnI GAAnn'nnTTC
Cuts at: 0 487 909
Size: 487 422
Enzymes that do cut:
AcuI AflIII AlfI AlwNI BanI BbvCI BglI BglII
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BseYI BsgI
BsmI Bsp1286I BspHI BsrDI BssSI BstEII BstXI BstYI
Bsu36I BtgI EaeI EarI Eco57MI EcoO109I MmeI MscI
MslI NcoI NdeI NspI PciI PflMI PfoI PpuMI
PstI SanDI SapI SexAI SfcI SmlI StyI TatI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI ZraI