(Linear) (Six-base) MAPSORT of: KIEE5374.seq  Check: 1616  from: 1  to: 897



KIEE5374.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:57  ..

ApoI r'AATT_y

Cuts at:      0      90     137     897 
   Size:         90      47     760

  Fragments arranged by size: 

                 760      90      47
 
AvrII C'CTAG_G

Cuts at:      0     754     897 
   Size:        754     143
 
BbvCI CC'TCA_GC

Cuts at:      0     126     304     897 
   Size:        126     178     593

  Fragments arranged by size: 

                 593     178     126
 
BglII A'GATC_T

Cuts at:      0     119     651     897 
   Size:        119     532     246

  Fragments arranged by size: 

                 532     246     119
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     111     497     897 
   Size:        111     386     400

  Fragments arranged by size: 

                 400     386     111
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     897 
   Size:        144     753
 
Bpu10I CC'TnA_GC

Cuts at:      0     126     304     897 
   Size:        126     178     593

  Fragments arranged by size: 

                 593     178     126
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     601     897 
   Size:        307     294     296

  Fragments arranged by size: 

                 307     296     294
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     162     897 
   Size:        162     735
 
BsaBI GATnn'nnATC

Cuts at:      0     592     897 
   Size:        592     305
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     114     381     897 
   Size:        114     267     516

  Fragments arranged by size: 

                 516     267     114
 
BsmI GAATG_Cn'

Cuts at:      0     817     897 
   Size:        817      80
 
BstYI r'GATC_y

Cuts at:      0     119     651     897 
   Size:        119     532     246

  Fragments arranged by size: 

                 532     246     119
 
BtgI C'CryG_G

Cuts at:      0     633     897 
   Size:        633     264
 
BtsI GCAGTG_nn'

Cuts at:      0     143     897 
   Size:        143     754
 
DraI TTT'AAA

Cuts at:      0     362     897 
   Size:        362     535
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     778     897 
   Size:        778     119
 
EarI CTCTTCn'nnn_

Cuts at:      0     103     897 
   Size:        103     794
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     897 
   Size:        144     753
 
EcoRV GAT'ATC

Cuts at:      0     578     897 
   Size:        578     319
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     263     897 
   Size:        263     634
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     867     897 
   Size:        867      30
 
HindIII A'AGCT_T

Cuts at:      0     677     897 
   Size:        677     220
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     780     897 
   Size:        365     415     117

  Fragments arranged by size: 

                 415     365     117
 
MslI CAynn'nnrTG

Cuts at:      0     388     897 
   Size:        388     509
 
MspA1I CmG'CkG

Cuts at:      0     130     897 
   Size:        130     767
 
NcoI C'CATG_G

Cuts at:      0     633     897 
   Size:        633     264
 
NspI r_CATG'y

Cuts at:      0     288     897 
   Size:        288     609
 
PsiI TTA'TAA

Cuts at:      0     254     897 
   Size:        254     643
 
PvuII CAG'CTG

Cuts at:      0     130     897 
   Size:        130     767
 
SmlI C'TyrA_G

Cuts at:      0     322     616     897 
   Size:        322     294     281

  Fragments arranged by size: 

                 322     294     281
 
StyI C'CwwG_G

Cuts at:      0     633     662     754     897 
   Size:        633      29      92     143

  Fragments arranged by size: 

                 633     143      92      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      93     897 
   Size:         93     804
 
TatI w'GTAC_w

Cuts at:      0     513     897 
   Size:        513     384
 
XmnI GAAnn'nnTTC

Cuts at:      0     246     897 
   Size:        246     651
 

 Enzymes that do cut:

     ApoI    AvrII    BbvCI    BglII     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BseRI     BsmI    BstYI     BtgI     BtsI     DraI
     DrdI     EarI  Eco57MI    EcoRV     FalI    Hin4I  HindIII     MmeI
     MslI   MspA1I     NcoI     NspI     PsiI    PvuII     SmlI     StyI
    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmtI     BplI    BsaAI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI   DraIII     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI