(Linear) (Six-base) MAPSORT of: KIEE5373.seq  Check: 7225  from: 1  to: 897



KIEE5373.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:57  ..

ApaI G_GGCC'C

Cuts at:      0     407     897 
   Size:        407     490
 
ApoI r'AATT_y

Cuts at:      0     137     897 
   Size:        137     760
 
BanII G_rGCy'C

Cuts at:      0     407     897 
   Size:        407     490
 
BbvCI CC'TCA_GC

Cuts at:      0     126     304     897 
   Size:        126     178     593

  Fragments arranged by size: 

                 593     178     126
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     821     897 
   Size:        821      76
 
BglII A'GATC_T

Cuts at:      0     119     897 
   Size:        119     778
 
BlpI GC'TnA_GC

Cuts at:      0     131     897 
   Size:        131     766
 
Bme1580I G_kGCm'C

Cuts at:      0     407     897 
   Size:        407     490
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     111     897 
   Size:        111     786
 
BmtI G_CTAG'C

Cuts at:      0     302     897 
   Size:        302     595
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     897 
   Size:        144     753
 
Bpu10I CC'TnA_GC

Cuts at:      0     126     304     897 
   Size:        126     178     593

  Fragments arranged by size: 

                 593     178     126
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     601     632     897 
   Size:        307     294      31     265

  Fragments arranged by size: 

                 307     294     265      31
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     162     897 
   Size:        162     735
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     764     794     897 
   Size:        764      30     103

  Fragments arranged by size: 

                 764     103      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     114     897 
   Size:        114     783
 
BseYI C'CCAG_C

Cuts at:      0     655     897 
   Size:        655     242
 
BsmI GAATG_Cn'

Cuts at:      0     513     897 
   Size:        513     384
 
Bsp1286I G_dGCh'C

Cuts at:      0     407     897 
   Size:        407     490
 
BstYI r'GATC_y

Cuts at:      0     119     643     651     897 
   Size:        119     524       8     246

  Fragments arranged by size: 

                 524     246     119       8
 
BtgI C'CryG_G

Cuts at:      0     443     897 
   Size:        443     454
 
BtsI GCAGTG_nn'

Cuts at:      0     143     897 
   Size:        143     754
 
EarI CTCTTCn'nnn_

Cuts at:      0     361     897 
   Size:        361     536
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     897 
   Size:        144     753
 
EcoO109I rG'GnC_Cy

Cuts at:      0     403     404     897 
   Size:        403       1     493

  Fragments arranged by size: 

                 493     403       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     411     443     897 
   Size:        411      32     454

  Fragments arranged by size: 

                 454     411      32
 
HincII GTy'rAC

Cuts at:      0     108     897 
   Size:        108     789
 
HindIII A'AGCT_T

Cuts at:      0     525     677     897 
   Size:        525     152     220

  Fragments arranged by size: 

                 525     220     152
 
HpaI GTT'AAC

Cuts at:      0     108     897 
   Size:        108     789
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     897 
   Size:        365     532
 
MspA1I CmG'CkG

Cuts at:      0     130     897 
   Size:        130     767
 
NcoI C'CATG_G

Cuts at:      0     443     897 
   Size:        443     454
 
NheI G'CTAG_C

Cuts at:      0     298     897 
   Size:        298     599
 
NspI r_CATG'y

Cuts at:      0     288     897 
   Size:        288     609
 
PspOMI G'GGCC_C

Cuts at:      0     403     897 
   Size:        403     494
 
PvuII CAG'CTG

Cuts at:      0     130     897 
   Size:        130     767
 
SmlI C'TyrA_G

Cuts at:      0     322     616     647     897 
   Size:        322     294      31     250

  Fragments arranged by size: 

                 322     294     250      31
 
StyI C'CwwG_G

Cuts at:      0     407     443     662     897 
   Size:        407      36     219     235

  Fragments arranged by size: 

                 407     235     219      36
 
TatI w'GTAC_w

Cuts at:      0     777     897 
   Size:        777     120
 
XmnI GAAnn'nnTTC

Cuts at:      0     277     897 
   Size:        277     620
 

 Enzymes that do cut:

     ApaI     ApoI    BanII    BbvCI    BciVI    BglII     BlpI Bme1580I
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI
    BseYI     BsmI Bsp1286I    BstYI     BtgI     BtsI     EarI  Eco57MI
 EcoO109I    Hin4I   HincII  HindIII     HpaI     MmeI   MspA1I     NcoI
     NheI     NspI   PspOMI    PvuII     SmlI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI     BcgI     BclI    BfrBI     BglI    BmgBI     BplI
    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI