(Linear) (Six-base) MAPSORT of: KIEE5372.seq  Check: 6433  from: 1  to: 897



KIEE5372.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:57  ..

ApaLI G'TGCA_C

Cuts at:      0     662     897 
   Size:        662     235
 
ApoI r'AATT_y

Cuts at:      0     164     220     472     897 
   Size:        164      56     252     425

  Fragments arranged by size: 

                 425     252     164      56
 
BclI T'GATC_A

Cuts at:      0     104     897 
   Size:        104     793
 
Bme1580I G_kGCm'C

Cuts at:      0     666     897 
   Size:        666     231
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     401     897 
   Size:        401     496
 
BsaBI GATnn'nnATC

Cuts at:      0     205     897 
   Size:        205     692
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     865     897 
   Size:        865      32
 
BsiHKAI G_wGCw'C

Cuts at:      0     666     897 
   Size:        666     231
 
Bsp1286I G_dGCh'C

Cuts at:      0     666     897 
   Size:        666     231
 
BsrDI GCAATG_nn'

Cuts at:      0     556     897 
   Size:        556     341
 
BsrFI r'CCGG_y

Cuts at:      0     637     897 
   Size:        637     260
 
BstYI r'GATC_y

Cuts at:      0     132     897 
   Size:        132     765
 
Bsu36I CC'TnA_GG

Cuts at:      0     466     897 
   Size:        466     431
 
BtgI C'CryG_G

Cuts at:      0     379     897 
   Size:        379     518
 
BtsI GCAGTG_nn'

Cuts at:      0      44     717     897 
   Size:         44     673     180

  Fragments arranged by size: 

                 673     180      44
 
EaeI y'GGCC_r

Cuts at:      0     635     897 
   Size:        635     262
 
EarI CTCTTCn'nnn_

Cuts at:      0     346     747     897 
   Size:        346     401     150

  Fragments arranged by size: 

                 401     346     150
 
EcoRI G'AATT_C

Cuts at:      0     220     897 
   Size:        220     677
 
EcoRV GAT'ATC

Cuts at:      0     365     897 
   Size:        365     532
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     582     614     806     838     897 
   Size:        582      32     192      32      59

  Fragments arranged by size: 

                 582     192      59      32      32
 
MslI CAynn'nnrTG

Cuts at:      0      79      82     790     897 
   Size:         79       3     708     107

  Fragments arranged by size: 

                 708     107      79       3
 
MspA1I CmG'CkG

Cuts at:      0     129     897 
   Size:        129     768
 
NaeI GCC'GGC

Cuts at:      0     639     897 
   Size:        639     258
 
NcoI C'CATG_G

Cuts at:      0     379     897 
   Size:        379     518
 
NgoMIV G'CCGG_C

Cuts at:      0     637     897 
   Size:        637     260
 
PsiI TTA'TAA

Cuts at:      0     262     897 
   Size:        262     635
 
PstI C_TGCA'G

Cuts at:      0     176     897 
   Size:        176     721
 
SfcI C'TryA_G

Cuts at:      0     172     897 
   Size:        172     725
 
StyI C'CwwG_G

Cuts at:      0     314     379     897 
   Size:        314      65     518

  Fragments arranged by size: 

                 518     314      65
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     350     681     897 
   Size:        350     331     216

  Fragments arranged by size: 

                 350     331     216
 
XbaI T'CTAG_A

Cuts at:      0     160     897 
   Size:        160     737
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     344     897 
   Size:        344     553
 
XmnI GAAnn'nnTTC

Cuts at:      0     475     897 
   Size:        475     422
 

 Enzymes that do cut:

    ApaLI     ApoI     BclI Bme1580I     BsaI    BsaBI    BseRI  BsiHKAI
 Bsp1286I    BsrDI    BsrFI    BstYI   Bsu36I     BtgI     BtsI     EaeI
     EarI    EcoRI    EcoRV    Hin4I     MslI   MspA1I     NaeI     NcoI
   NgoMIV     PsiI     PstI     SfcI     StyI    TaqII     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NarI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XhoI     XmaI     ZraI