(Linear) (Six-base) MAPSORT of: KIEE5366.seq  Check: 4613  from: 1  to: 570



KIEE5366.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:57  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     348     570 
   Size:        348     222
 
ApoI r'AATT_y

Cuts at:      0     322     570 
   Size:        322     248
 
BglII A'GATC_T

Cuts at:      0     260     570 
   Size:        260     310
 
BlpI GC'TnA_GC

Cuts at:      0      43     570 
   Size:         43     527
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     570 
   Size:        209     361
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      79     570 
   Size:         79     491
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     208     394     570 
   Size:        208     186     176

  Fragments arranged by size: 

                 208     186     176
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     312     570 
   Size:        312     258
 
BsiHKAI G_wGCw'C

Cuts at:      0      44     570 
   Size:         44     526
 
BsmI GAATG_Cn'

Cuts at:      0     414     570 
   Size:        414     156
 
Bsp1286I G_dGCh'C

Cuts at:      0      44     570 
   Size:         44     526
 
BspHI T'CATG_A

Cuts at:      0     515     570 
   Size:        515      55
 
BstYI r'GATC_y

Cuts at:      0     260     520     570 
   Size:        260     260      50

  Fragments arranged by size: 

                 260     260      50
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     570 
   Size:        209     361
 
EcoO109I rG'GnC_Cy

Cuts at:      0     110     309     570 
   Size:        110     199     261

  Fragments arranged by size: 

                 261     199     110
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     456     570 
   Size:        456     114
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     497     529     570 
   Size:        497      32      41

  Fragments arranged by size: 

                 497      41      32
 
MspA1I CmG'CkG

Cuts at:      0      47     348     570 
   Size:         47     301     222

  Fragments arranged by size: 

                 301     222      47
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      96     570 
   Size:         96     474
 
PpuMI rG'GwC_Cy

Cuts at:      0     110     309     570 
   Size:        110     199     261

  Fragments arranged by size: 

                 261     199     110
 
PvuII CAG'CTG

Cuts at:      0      47     348     570 
   Size:         47     301     222

  Fragments arranged by size: 

                 301     222      47
 
XmnI GAAnn'nnTTC

Cuts at:      0     439     570 
   Size:        439     131
 

 Enzymes that do cut:

    AlwNI     ApoI    BglII     BlpI     BpmI     BsaI    BseRI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BstYI  Eco57MI EcoO109I     FalI
    Hin4I   MspA1I    PflMI    PpuMI    PvuII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI