Restriction map for
pFN21AE5364
(Linear) (Six-base) MAPSORT of: KIEE5364.seq Check: 8601 from: 1 to: 897
KIEE5364.orf
With 182 enzymes: SgfI *
January 29, 2014 11:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 897
Size: 225 672
AflIII A'CryG_T
Cuts at: 0 545 897
Size: 545 352
AleI CACnn'nnGTG
Cuts at: 0 800 897
Size: 800 97
BanI G'GyrC_C
Cuts at: 0 54 143 897
Size: 54 89 754
Fragments arranged by size:
754 89 54
BanII G_rGCy'C
Cuts at: 0 819 868 897
Size: 819 49 29
Fragments arranged by size:
819 49 29
BbeI G_GCGC'C
Cuts at: 0 147 897
Size: 147 750
BbsI GAAGACnn'nnnn_
Cuts at: 0 63 897
Size: 63 834
BbvCI CC'TCA_GC
Cuts at: 0 270 897
Size: 270 627
BglI GCCn_nnn'nGGC
Cuts at: 0 889 897
Size: 889 8
BlpI GC'TnA_GC
Cuts at: 0 19 40 818 897
Size: 19 21 778 79
Fragments arranged by size:
778 79 21 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 461 610 897
Size: 461 149 287
Fragments arranged by size:
461 287 149
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 601 897
Size: 27 574 296
Fragments arranged by size:
574 296 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 580 739 852 897
Size: 237 343 159 113 45
Fragments arranged by size:
343 237 159 113 45
Bpu10I CC'TnA_GC
Cuts at: 0 270 897
Size: 270 627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 897
Size: 94 803
BsaI GGTCTCn'nnnn_
Cuts at: 0 520 897
Size: 520 377
BsaHI Gr'CG_yC
Cuts at: 0 144 897
Size: 144 753
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 865 897
Size: 865 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 897
Size: 526 371
BsiHKAI G_wGCw'C
Cuts at: 0 819 897
Size: 819 78
BsmI GAATG_Cn'
Cuts at: 0 822 897
Size: 822 75
BsmBI CGTCTCn'nnnn_
Cuts at: 0 99 897
Size: 99 798
Bsp1286I G_dGCh'C
Cuts at: 0 819 868 897
Size: 819 49 29
Fragments arranged by size:
819 49 29
BspHI T'CATG_A
Cuts at: 0 440 897
Size: 440 457
BsrBI CCG'CTC
Cuts at: 0 60 897
Size: 60 837
BsrDI GCAATG_nn'
Cuts at: 0 180 897
Size: 180 717
BstEII G'GTnAC_C
Cuts at: 0 763 897
Size: 763 134
BstXI CCAn_nnnn'nTGG
Cuts at: 0 802 897
Size: 802 95
BstYI r'GATC_y
Cuts at: 0 536 897
Size: 536 361
BtgI C'CryG_G
Cuts at: 0 426 783 897
Size: 426 357 114
Fragments arranged by size:
426 357 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 138 897
Size: 138 759
ClaI AT'CG_AT
Cuts at: 0 233 897
Size: 233 664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 768 803 897
Size: 768 35 94
Fragments arranged by size:
768 94 35
EaeI y'GGCC_r
Cuts at: 0 356 827 897
Size: 356 471 70
Fragments arranged by size:
471 356 70
EarI CTCTTCn'nnn_
Cuts at: 0 67 316 592 897
Size: 67 249 276 305
Fragments arranged by size:
305 276 249 67
EcoICRI GAG'CTC
Cuts at: 0 817 897
Size: 817 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 237 580 739 852 897
Size: 225 12 343 159 113 45
Fragments arranged by size:
343 225 159 113 45 12
EcoO109I rG'GnC_Cy
Cuts at: 0 791 897
Size: 791 106
HaeII r_GCGC'y
Cuts at: 0 19 40 147 897
Size: 19 21 107 750
Fragments arranged by size:
750 107 21 19
KasI G'GCGC_C
Cuts at: 0 143 897
Size: 143 754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 897
Size: 512 385
MscI TGG'CCA
Cuts at: 0 358 897
Size: 358 539
MslI CAynn'nnrTG
Cuts at: 0 352 800 897
Size: 352 448 97
Fragments arranged by size:
448 352 97
MspA1I CmG'CkG
Cuts at: 0 274 501 608 800 897
Size: 274 227 107 192 97
Fragments arranged by size:
274 227 192 107 97
NarI GG'CG_CC
Cuts at: 0 144 897
Size: 144 753
NcoI C'CATG_G
Cuts at: 0 783 897
Size: 783 114
NspI r_CATG'y
Cuts at: 0 549 897
Size: 549 348
PciI A'CATG_T
Cuts at: 0 545 897
Size: 545 352
PfoI T'CCnGG_A
Cuts at: 0 868 897
Size: 868 29
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 23 55 897
Size: 23 32 842
Fragments arranged by size:
842 32 23
PpuMI rG'GwC_Cy
Cuts at: 0 791 897
Size: 791 106
PvuII CAG'CTG
Cuts at: 0 274 501 897
Size: 274 227 396
Fragments arranged by size:
396 274 227
SacI G_AGCT'C
Cuts at: 0 819 897
Size: 819 78
SanDI GG'GwC_CC
Cuts at: 0 791 897
Size: 791 106
SapI GCTCTTCn'nnn_
Cuts at: 0 67 897
Size: 67 830
SfoI GGC'GCC
Cuts at: 0 145 897
Size: 145 752
SmlI C'TyrA_G
Cuts at: 0 109 897
Size: 109 788
StyI C'CwwG_G
Cuts at: 0 216 560 783 845 897
Size: 216 344 223 62 52
Fragments arranged by size:
344 223 216 62 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 443 703 897
Size: 443 260 194
Fragments arranged by size:
443 260 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 366 781 802 897
Size: 261 105 415 21 95
Fragments arranged by size:
415 261 105 95 21
Enzymes that do cut:
AcuI AflIII AleI BanI BanII BbeI BbsI BbvCI
BglI BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BsrBI BsrDI BstEII BstXI BstYI BtgI BtgZI ClaI
CspCI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII KasI
MmeI MscI MslI MspA1I NarI NcoI NspI PciI
PfoI PpiI PpuMI PvuII SacI SanDI SapI SfoI
SmlI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BcgI BciVI
BclI BfrBI BglII Bme1580I BmgBI BmtI BsaAI BsaBI
BsaWI BsaXI BseYI BsiEI BsiWI BspEI BspMI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtsI
DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI