(Linear) (Six-base) MAPSORT of: KIEE5362.seq  Check: 6495  from: 1  to: 825



KIEE5362.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     169     825 
   Size:        169     656
 
AfeI AGC'GCT

Cuts at:      0     269     825 
   Size:        269     556
 
ApoI r'AATT_y

Cuts at:      0     277     334     825 
   Size:        277      57     491

  Fragments arranged by size: 

                 491     277      57
 
AvaI C'yCGr_G

Cuts at:      0       8     125     825 
   Size:          8     117     700

  Fragments arranged by size: 

                 700     117       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     786     825 
   Size:        786      39
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     593     825 
   Size:        593     232
 
Bme1580I G_kGCm'C

Cuts at:      0     639     825 
   Size:        639     186
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     182     825 
   Size:        182     643
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     825 
   Size:         43     782
 
BsaHI Gr'CG_yC

Cuts at:      0     121     825 
   Size:        121     704
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      37      82     825 
   Size:         37      45     743

  Fragments arranged by size: 

                 743      45      37
 
BsiEI CG_ry'CG

Cuts at:      0      92     156     825 
   Size:         92      64     669

  Fragments arranged by size: 

                 669      92      64
 
BsiWI C'GTAC_G

Cuts at:      0      17     825 
   Size:         17     808
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       7      61     825 
   Size:          7      54     764

  Fragments arranged by size: 

                 764      54       7
 
Bsp1286I G_dGCh'C

Cuts at:      0     639     825 
   Size:        639     186
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     169     825 
   Size:        169     656
 
BsrDI GCAATG_nn'

Cuts at:      0     395     614     825 
   Size:        395     219     211

  Fragments arranged by size: 

                 395     219     211
 
BsrGI T'GTAC_A

Cuts at:      0     392     825 
   Size:        392     433
 
BssSI C'ACGA_G

Cuts at:      0     259     825 
   Size:        259     566
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     152     825 
   Size:        152     673
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     509     544     825 
   Size:        509      35     281

  Fragments arranged by size: 

                 509     281      35
 
DraI TTT'AAA

Cuts at:      0     315     825 
   Size:        315     510
 
EaeI y'GGCC_r

Cuts at:      0      89     153     448     712     825 
   Size:         89      64     295     264     113

  Fragments arranged by size: 

                 295     264     113      89      64
 
EagI C'GGCC_G

Cuts at:      0      89     153     825 
   Size:         89      64     672

  Fragments arranged by size: 

                 672      89      64
 
HaeII r_GCGC'y

Cuts at:      0     271     825 
   Size:        271     554
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     454     486     607     639     825 
   Size:        454      32     121      32     186

  Fragments arranged by size: 

                 454     186     121      32      32
 
HincII GTy'rAC

Cuts at:      0     406     825 
   Size:        406     419
 
PstI C_TGCA'G

Cuts at:      0     381     825 
   Size:        381     444
 
SfcI C'TryA_G

Cuts at:      0     377     825 
   Size:        377     448
 
SmaI CCC'GGG

Cuts at:      0     127     825 
   Size:        127     698
 
SmlI C'TyrA_G

Cuts at:      0      58     825 
   Size:         58     767
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      79     825 
   Size:         79     746
 
TatI w'GTAC_w

Cuts at:      0     392     812     825 
   Size:        392     420      13

  Fragments arranged by size: 

                 420     392      13
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     200     232     825 
   Size:        200      32     593

  Fragments arranged by size: 

                 593     200      32
 
XmaI C'CCGG_G

Cuts at:      0     125     825 
   Size:        125     700
 

 Enzymes that do cut:

     AarI     AfeI     ApoI     AvaI     BbsI    BciVI Bme1580I     BplI
    BpuEI    BsaHI    BseRI    BsiEI    BsiWI    BsmBI Bsp1286I    BspMI
    BsrDI    BsrGI    BssSI    BtgZI    CspCI     DraI     EaeI     EagI
    HaeII    Hin4I   HincII     PstI     SfcI     SmaI     SmlI    TaqII
     TatI     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseYI     BsgI  BsiHKAI     BsmI    BspEI    BspHI    BsrBI
    BsrFI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI   DraIII     DrdI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI