Restriction map for
pFN21AE5361
(Linear) (Six-base) MAPSORT of: KIEE5361.seq Check: 3780 from: 1 to: 912
KIEE5361.orf
With 182 enzymes: SgfI *
January 29, 2014 11:57 ..
AccI GT'mk_AC
Cuts at: 0 744 904 912
Size: 744 160 8
Fragments arranged by size:
744 160 8
AclI AA'CG_TT
Cuts at: 0 306 912
Size: 306 606
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 688 912
Size: 593 95 224
Fragments arranged by size:
593 224 95
AflIII A'CryG_T
Cuts at: 0 182 298 912
Size: 182 116 614
Fragments arranged by size:
614 182 116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 442 912
Size: 442 470
ApaLI G'TGCA_C
Cuts at: 0 508 912
Size: 508 404
ApoI r'AATT_y
Cuts at: 0 350 726 912
Size: 350 376 186
Fragments arranged by size:
376 350 186
AvaI C'yCGr_G
Cuts at: 0 220 912
Size: 220 692
BanI G'GyrC_C
Cuts at: 0 200 912
Size: 200 712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 609 643 912
Size: 609 34 269
Fragments arranged by size:
609 269 34
BlpI GC'TnA_GC
Cuts at: 0 770 912
Size: 770 142
Bme1580I G_kGCm'C
Cuts at: 0 512 912
Size: 512 400
BmgBI CAC'GTC
Cuts at: 0 216 299 623 912
Size: 216 83 324 289
Fragments arranged by size:
324 289 216 83
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 912
Size: 267 645
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 612 912
Size: 139 473 300
Fragments arranged by size:
473 300 139
BsaWI w'CCGG_w
Cuts at: 0 603 912
Size: 603 309
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 912
Size: 142 770
BsiEI CG_ry'CG
Cuts at: 0 116 912
Size: 116 796
BsiHKAI G_wGCw'C
Cuts at: 0 47 221 512 912
Size: 47 174 291 400
Fragments arranged by size:
400 291 174 47
BsiWI C'GTAC_G
Cuts at: 0 17 912
Size: 17 895
Bsp1286I G_dGCh'C
Cuts at: 0 47 221 512 912
Size: 47 174 291 400
Fragments arranged by size:
400 291 174 47
BspEI T'CCGG_A
Cuts at: 0 603 912
Size: 603 309
BspHI T'CATG_A
Cuts at: 0 780 912
Size: 780 132
BsrDI GCAATG_nn'
Cuts at: 0 764 912
Size: 764 148
BsrFI r'CCGG_y
Cuts at: 0 76 115 912
Size: 76 39 797
Fragments arranged by size:
797 76 39
BstAPI GCAn_nnn'nTGC
Cuts at: 0 416 826 912
Size: 416 410 86
Fragments arranged by size:
416 410 86
BstZ17I GTA'TAC
Cuts at: 0 745 905 912
Size: 745 160 7
Fragments arranged by size:
745 160 7
BtgI C'CryG_G
Cuts at: 0 209 912
Size: 209 703
DrdI GACnn_nn'nnGTC
Cuts at: 0 877 912
Size: 877 35
EaeI y'GGCC_r
Cuts at: 0 113 912
Size: 113 799
EagI C'GGCC_G
Cuts at: 0 113 912
Size: 113 799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 593 688 912
Size: 267 326 95 224
Fragments arranged by size:
326 267 224 95
EcoNI CCTnn'n_nnAGG
Cuts at: 0 157 912
Size: 157 755
EcoO109I rG'GnC_Cy
Cuts at: 0 147 912
Size: 147 765
EcoRI G'AATT_C
Cuts at: 0 350 726 912
Size: 350 376 186
Fragments arranged by size:
376 350 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 585 607 617 635 639 667 912
Size: 585 22 10 18 4 28 245
Fragments arranged by size:
585 245 28 22 18 10 4
MluI A'CGCG_T
Cuts at: 0 182 912
Size: 182 730
MslI CAynn'nnrTG
Cuts at: 0 424 912
Size: 424 488
MspA1I CmG'CkG
Cuts at: 0 211 912
Size: 211 701
NaeI GCC'GGC
Cuts at: 0 78 117 912
Size: 78 39 795
Fragments arranged by size:
795 78 39
NgoMIV G'CCGG_C
Cuts at: 0 76 115 912
Size: 76 39 797
Fragments arranged by size:
797 76 39
NspI r_CATG'y
Cuts at: 0 687 833 912
Size: 687 146 79
Fragments arranged by size:
687 146 79
PasI CC'CwG_GG
Cuts at: 0 71 912
Size: 71 841
SacII CC_GC'GG
Cuts at: 0 212 912
Size: 212 700
SmlI C'TyrA_G
Cuts at: 0 154 591 912
Size: 154 437 321
Fragments arranged by size:
437 321 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 912
Size: 79 833
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 169 201 833 865 912
Size: 169 32 632 32 47
Fragments arranged by size:
632 169 47 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 136 912
Size: 136 776
Enzymes that do cut:
AccI AclI AcuI AflIII AlfI ApaLI ApoI AvaI
BanI BcgI BlpI Bme1580I BmgBI BpmI BpuEI BsaWI
BseRI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BsrDI
BsrFI BstAPI BstZ17I BtgI DrdI EaeI EagI Eco57MI
EcoNI EcoO109I EcoRI Hin4I MluI MslI MspA1I NaeI
NgoMIV NspI PasI SacII SmlI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaXI BseYI BsgI BsmI BsmBI BspMI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EarI
EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MmeI MscI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI ZraI