Restriction map for
pFN21AE5359
(Linear) (Six-base) MAPSORT of: KIEE5359.seq Check: 3413 from: 1 to: 921
KIEE5359.orf
With 182 enzymes: SgfI *
January 29, 2014 11:57 ..
AatII G_ACGT'C
Cuts at: 0 148 635 921
Size: 148 487 286
Fragments arranged by size:
487 286 148
Acc65I G'GTAC_C
Cuts at: 0 813 921
Size: 813 108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 921
Size: 229 692
AleI CACnn'nnGTG
Cuts at: 0 190 921
Size: 190 731
AseI AT'TA_AT
Cuts at: 0 722 921
Size: 722 199
BamHI G'GATC_C
Cuts at: 0 835 921
Size: 835 86
BanI G'GyrC_C
Cuts at: 0 813 921
Size: 813 108
BclI T'GATC_A
Cuts at: 0 86 921
Size: 86 835
BglI GCCn_nnn'nGGC
Cuts at: 0 240 921
Size: 240 681
Bme1580I G_kGCm'C
Cuts at: 0 755 921
Size: 755 166
BmgBI CAC'GTC
Cuts at: 0 84 921
Size: 84 837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 389 921
Size: 106 283 532
Fragments arranged by size:
532 283 106
BsaHI Gr'CG_yC
Cuts at: 0 145 632 921
Size: 145 487 289
Fragments arranged by size:
487 289 145
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 365 395 921
Size: 365 30 526
Fragments arranged by size:
526 365 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 386 921
Size: 386 535
BsmI GAATG_Cn'
Cuts at: 0 142 921
Size: 142 779
Bsp1286I G_dGCh'C
Cuts at: 0 755 921
Size: 755 166
BsrDI GCAATG_nn'
Cuts at: 0 770 921
Size: 770 151
BsrGI T'GTAC_A
Cuts at: 0 565 921
Size: 565 356
BstYI r'GATC_y
Cuts at: 0 835 921
Size: 835 86
BtgI C'CryG_G
Cuts at: 0 441 921
Size: 441 480
DraIII CAC_nnn'GTG
Cuts at: 0 648 921
Size: 648 273
EaeI y'GGCC_r
Cuts at: 0 107 921
Size: 107 814
EarI CTCTTCn'nnn_
Cuts at: 0 55 921
Size: 55 866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 94 225 252 921
Size: 94 131 27 669
Fragments arranged by size:
669 131 94 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 229 389 921
Size: 106 123 160 532
Fragments arranged by size:
532 160 123 106
EcoNI CCTnn'n_nnAGG
Cuts at: 0 270 335 921
Size: 270 65 586
Fragments arranged by size:
586 270 65
EcoO109I rG'GnC_Cy
Cuts at: 0 6 921
Size: 6 915
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 436 618 921
Size: 436 182 303
Fragments arranged by size:
436 303 182
FspI TGC'GCA
Cuts at: 0 675 921
Size: 675 246
KpnI G_GTAC'C
Cuts at: 0 817 921
Size: 817 104
MfeI C'AATT_G
Cuts at: 0 561 921
Size: 561 360
MslI CAynn'nnrTG
Cuts at: 0 190 921
Size: 190 731
MspA1I CmG'CkG
Cuts at: 0 58 99 190 227 249 921
Size: 58 41 91 37 22 672
Fragments arranged by size:
672 91 58 41 37 22
NdeI CA'TA_TG
Cuts at: 0 582 921
Size: 582 339
PasI CC'CwG_GG
Cuts at: 0 36 921
Size: 36 885
PfoI T'CCnGG_A
Cuts at: 0 296 838 921
Size: 296 542 83
Fragments arranged by size:
542 296 83
PsiI TTA'TAA
Cuts at: 0 473 921
Size: 473 448
PvuII CAG'CTG
Cuts at: 0 58 921
Size: 58 863
SapI GCTCTTCn'nnn_
Cuts at: 0 55 921
Size: 55 866
SfcI C'TryA_G
Cuts at: 0 222 921
Size: 222 699
StuI AGG'CCT
Cuts at: 0 601 921
Size: 601 320
StyI C'CwwG_G
Cuts at: 0 129 921
Size: 129 792
TatI w'GTAC_w
Cuts at: 0 565 740 921
Size: 565 175 181
Fragments arranged by size:
565 181 175
Tth111I GACn'n_nGTC
Cuts at: 0 292 921
Size: 292 629
XmnI GAAnn'nnTTC
Cuts at: 0 772 921
Size: 772 149
ZraI GAC'GTC
Cuts at: 0 146 633 921
Size: 146 487 288
Fragments arranged by size:
487 288 146
Enzymes that do cut:
AatII Acc65I AcuI AleI AseI BamHI BanI BclI
BglI Bme1580I BmgBI BpmI BsaHI BsaXI BseRI BsmI
Bsp1286I BsrDI BsrGI BstYI BtgI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I FalI FspI KpnI MfeI
MslI MspA1I NdeI PasI PfoI PsiI PvuII SapI
SfcI StuI StyI TatI Tth111I XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AsiSI
AvaI AvrII BaeI BanII BbeI BbsI BbvCI BcgI
BciVI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EagI EcoICRI
EcoRI EcoRV FseI FspAI HaeII Hin4I HincII HindIII
HpaI KasI MluI MmeI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TstI XbaI XcmI XhoI XmaI