Restriction map for
pFN21AE5355
(Linear) (Six-base) MAPSORT of: KIEE5355.seq Check: 7258 from: 1 to: 882
KIEE5355.orf
With 182 enzymes: SgfI *
January 29, 2014 11:57 ..
Acc65I G'GTAC_C
Cuts at: 0 670 882
Size: 670 212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 882
Size: 629 253
AlwNI CAG_nnn'CTG
Cuts at: 0 184 783 882
Size: 184 599 99
Fragments arranged by size:
599 184 99
ApoI r'AATT_y
Cuts at: 0 151 598 882
Size: 151 447 284
Fragments arranged by size:
447 284 151
AseI AT'TA_AT
Cuts at: 0 836 882
Size: 836 46
BamHI G'GATC_C
Cuts at: 0 714 882
Size: 714 168
BanI G'GyrC_C
Cuts at: 0 670 882
Size: 670 212
BanII G_rGCy'C
Cuts at: 0 863 882
Size: 863 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 302 371 882
Size: 302 69 511
Fragments arranged by size:
511 302 69
BglII A'GATC_T
Cuts at: 0 633 882
Size: 633 249
BmrI ACTGGGnnnn_n'
Cuts at: 0 206 566 882
Size: 206 360 316
Fragments arranged by size:
360 316 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 882
Size: 863 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 202 882
Size: 202 680
BsaWI w'CCGG_w
Cuts at: 0 163 882
Size: 163 719
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 663 882
Size: 663 219
Bsp1286I G_dGCh'C
Cuts at: 0 863 882
Size: 863 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 447 882
Size: 447 435
BsrDI GCAATG_nn'
Cuts at: 0 692 882
Size: 692 190
BssHII G'CGCG_C
Cuts at: 0 23 882
Size: 23 859
BstXI CCAn_nnnn'nTGG
Cuts at: 0 856 882
Size: 856 26
BstYI r'GATC_y
Cuts at: 0 633 714 882
Size: 633 81 168
Fragments arranged by size:
633 168 81
BtgI C'CryG_G
Cuts at: 0 9 447 882
Size: 9 438 435
Fragments arranged by size:
438 435 9
BtsI GCAGTG_nn'
Cuts at: 0 147 548 719 882
Size: 147 401 171 163
Fragments arranged by size:
401 171 163 147
EarI CTCTTCn'nnn_
Cuts at: 0 823 882
Size: 823 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 863 882
Size: 629 234 19
Fragments arranged by size:
629 234 19
EcoO109I rG'GnC_Cy
Cuts at: 0 200 882
Size: 200 682
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 428 460 882
Size: 428 32 422
Fragments arranged by size:
428 422 32
HincII GTy'rAC
Cuts at: 0 420 744 882
Size: 420 324 138
Fragments arranged by size:
420 324 138
HindIII A'AGCT_T
Cuts at: 0 799 882
Size: 799 83
KpnI G_GTAC'C
Cuts at: 0 674 882
Size: 674 208
MfeI C'AATT_G
Cuts at: 0 290 882
Size: 290 592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 854 882
Size: 692 162 28
Fragments arranged by size:
692 162 28
MslI CAynn'nnrTG
Cuts at: 0 578 882
Size: 578 304
MspA1I CmG'CkG
Cuts at: 0 457 882
Size: 457 425
NcoI C'CATG_G
Cuts at: 0 447 882
Size: 447 435
NdeI CA'TA_TG
Cuts at: 0 51 882
Size: 51 831
PpuMI rG'GwC_Cy
Cuts at: 0 200 882
Size: 200 682
PvuII CAG'CTG
Cuts at: 0 457 882
Size: 457 425
SapI GCTCTTCn'nnn_
Cuts at: 0 823 882
Size: 823 59
SfcI C'TryA_G
Cuts at: 0 549 882
Size: 549 333
StuI AGG'CCT
Cuts at: 0 789 882
Size: 789 93
StyI C'CwwG_G
Cuts at: 0 60 203 405 447 882
Size: 60 143 202 42 435
Fragments arranged by size:
435 202 143 60 42
Enzymes that do cut:
Acc65I AcuI AlwNI ApoI AseI BamHI BanI BanII
BbsI BglII BmrI BpmI BsaI BsaWI BsgI Bsp1286I
BspMI BsrDI BssHII BstXI BstYI BtgI BtsI EarI
Eco57MI EcoO109I Hin4I HincII HindIII KpnI MfeI MmeI
MslI MspA1I NcoI NdeI PpuMI PvuII SapI SfcI
StuI StyI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HpaI KasI MluI MscI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI