Restriction map for
pFN21AE5353
(Linear) (Six-base) MAPSORT of: KIEE5353.seq Check: 2153 from: 1 to: 975
KIEE5353.orf
With 182 enzymes: SgfI *
January 29, 2014 11:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 597 833 975
Size: 597 236 142
Fragments arranged by size:
597 236 142
Acc65I G'GTAC_C
Cuts at: 0 840 975
Size: 840 135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 481 619 975
Size: 244 237 138 356
Fragments arranged by size:
356 244 237 138
AlwNI CAG_nnn'CTG
Cuts at: 0 853 975
Size: 853 122
ApoI r'AATT_y
Cuts at: 0 99 559 726 975
Size: 99 460 167 249
Fragments arranged by size:
460 249 167 99
AseI AT'TA_AT
Cuts at: 0 692 975
Size: 692 283
AvaI C'yCGr_G
Cuts at: 0 338 551 975
Size: 338 213 424
Fragments arranged by size:
424 338 213
BamHI G'GATC_C
Cuts at: 0 273 975
Size: 273 702
BanI G'GyrC_C
Cuts at: 0 840 975
Size: 840 135
BanII G_rGCy'C
Cuts at: 0 149 975
Size: 149 826
BbsI GAAGACnn'nnnn_
Cuts at: 0 232 632 975
Size: 232 400 343
Fragments arranged by size:
400 343 232
BbvCI CC'TCA_GC
Cuts at: 0 256 975
Size: 256 719
Bme1580I G_kGCm'C
Cuts at: 0 22 975
Size: 22 953
BmrI ACTGGGnnnn_n'
Cuts at: 0 705 975
Size: 705 270
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 566 975
Size: 566 409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 975
Size: 875 100
Bpu10I CC'TnA_GC
Cuts at: 0 256 975
Size: 256 719
BsaBI GATnn'nnATC
Cuts at: 0 278 975
Size: 278 697
BsaHI Gr'CG_yC
Cuts at: 0 239 975
Size: 239 736
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 586 691 975
Size: 373 213 105 284
Fragments arranged by size:
373 284 213 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 975
Size: 883 92
BsiEI CG_ry'CG
Cuts at: 0 646 975
Size: 646 329
BsiHKAI G_wGCw'C
Cuts at: 0 149 881 975
Size: 149 732 94
Fragments arranged by size:
732 149 94
Bsp1286I G_dGCh'C
Cuts at: 0 22 149 881 975
Size: 22 127 732 94
Fragments arranged by size:
732 127 94 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 597 833 975
Size: 597 236 142
Fragments arranged by size:
597 236 142
BsrDI GCAATG_nn'
Cuts at: 0 172 975
Size: 172 803
BsrFI r'CCGG_y
Cuts at: 0 344 975
Size: 344 631
BsrGI T'GTAC_A
Cuts at: 0 12 927 975
Size: 12 915 48
Fragments arranged by size:
915 48 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 609 975
Size: 609 366
BstYI r'GATC_y
Cuts at: 0 273 831 975
Size: 273 558 144
Fragments arranged by size:
558 273 144
BtgI C'CryG_G
Cuts at: 0 242 835 975
Size: 242 593 140
Fragments arranged by size:
593 242 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 310 975
Size: 310 665
DraI TTT'AAA
Cuts at: 0 422 975
Size: 422 553
EarI CTCTTCn'nnn_
Cuts at: 0 401 500 975
Size: 401 99 475
Fragments arranged by size:
475 401 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 908 975
Size: 908 67
EcoICRI GAG'CTC
Cuts at: 0 147 975
Size: 147 828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 481 619 875 975
Size: 244 237 138 256 100
Fragments arranged by size:
256 244 237 138 100
EcoNI CCTnn'n_nnAGG
Cuts at: 0 487 975
Size: 487 488
EcoO109I rG'GnC_Cy
Cuts at: 0 850 975
Size: 850 125
EcoRI G'AATT_C
Cuts at: 0 99 559 726 975
Size: 99 460 167 249
Fragments arranged by size:
460 249 167 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 87 119 648 680 975
Size: 87 32 529 32 295
Fragments arranged by size:
529 295 87 32 32
HincII GTy'rAC
Cuts at: 0 159 975
Size: 159 816
HindIII A'AGCT_T
Cuts at: 0 740 975
Size: 740 235
HpaI GTT'AAC
Cuts at: 0 159 975
Size: 159 816
KpnI G_GTAC'C
Cuts at: 0 844 975
Size: 844 131
MspA1I CmG'CkG
Cuts at: 0 244 354 975
Size: 244 110 621
Fragments arranged by size:
621 244 110
NcoI C'CATG_G
Cuts at: 0 835 975
Size: 835 140
NruI TCG'CGA
Cuts at: 0 329 975
Size: 329 646
PfoI T'CCnGG_A
Cuts at: 0 149 975
Size: 149 826
PstI C_TGCA'G
Cuts at: 0 353 975
Size: 353 622
PvuI CG_AT'CG
Cuts at: 0 646 975
Size: 646 329
SacI G_AGCT'C
Cuts at: 0 149 975
Size: 149 826
SacII CC_GC'GG
Cuts at: 0 245 975
Size: 245 730
SapI GCTCTTCn'nnn_
Cuts at: 0 500 975
Size: 500 475
SfcI C'TryA_G
Cuts at: 0 349 682 975
Size: 349 333 293
Fragments arranged by size:
349 333 293
SmaI CCC'GGG
Cuts at: 0 340 975
Size: 340 635
SmlI C'TyrA_G
Cuts at: 0 551 975
Size: 551 424
StyI C'CwwG_G
Cuts at: 0 835 975
Size: 835 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 896 975
Size: 896 79
TatI w'GTAC_w
Cuts at: 0 12 927 975
Size: 12 915 48
Fragments arranged by size:
915 48 12
XbaI T'CTAG_A
Cuts at: 0 25 975
Size: 25 950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 893 975
Size: 893 82
XhoI C'TCGA_G
Cuts at: 0 551 975
Size: 551 424
XmaI C'CCGG_G
Cuts at: 0 338 975
Size: 338 637
Enzymes that do cut:
AarI Acc65I AcuI AlwNI ApoI AseI AvaI BamHI
BanI BanII BbsI BbvCI Bme1580I BmrI BplI BpmI
Bpu10I BsaBI BsaHI BseRI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstAPI BstYI BtgI BtgZI
DraI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
Hin4I HincII HindIII HpaI KpnI MspA1I NcoI NruI
PfoI PstI PvuI SacI SacII SapI SfcI SmaI
SmlI StyI TaqII TatI XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaWI BsaXI
BseYI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI
CspCI DraIII DrdI EaeI EagI EcoRV FalI FseI
FspI FspAI HaeII KasI MfeI MluI MmeI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuII RsrII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XmnI ZraI